Infrared spectroscopy as a new tool for studying single living cells: Is there a niche?

Sabbatini, Simona; Conti, Carla; Orilisi, Giulia; Giorgini, Elisabetta

doi:10.3233/bsi-170171

Cited by 53 publications

(46 citation statements)

References 74 publications

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“…It was found that the water uptake was enhanced from 277 ± 22% for the PEUUF scaffold to 367 ± 5% for the PRP-PEUUF ones. The FTIR-ATR analysis of those PRP-PEUUF scaffolds presented the significant bands of PEUUF, and also exhibited bands from proteins present in PRP, such as the amide I and II groups of the peptide bond at respectively, 1650 cm −1 and 1542 cm −1 , the bending of CH 2/3 groups present in amino acid side chains at 1400 cm −1 , as well as the broadening of the band centered at 3293 cm −1 assigned to the protein NH groups (Figure 7) [45].…”

Section: Resultsmentioning

confidence: 99%

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

et al. 2019

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Due to their elastomeric behavior, polyurethane-based scaffolds can find various applications in soft-tissue engineering. However, their relatively inert surface has to be modified in order to improve cell colonization and control cell fate. The present study focuses on porous biodegradable scaffolds based on poly(ester-urea-urethane), functionalized concomitantly to the scaffold elaboration with low-molecular-weight (LMW) fucoidan; and their bio-activation with platelet rich plasma (PRP) formulations with the aim to promote cell response. The LMW fucoidan-functionalization was obtained in a very homogeneous way, and was stable after the scaffold sterilization and incubation in phosphate-buffered saline. Biomolecules from PRP readily penetrated into the functionalized scaffold, leading to a biological frame on the pore walls. Preliminary in vitro assays were assessed to demonstrate the improvement of scaffold behavior towards cell response. The scaffold bio-activation drastically improved cell migration. Moreover, cells interacted with all pore sides into the bio-activated scaffold forming cell bridges across pores. Our work brought out an easy and versatile way of developing functionalized and bio-activated elastomeric poly(ester-urea-urethane) scaffolds with a better cell response.

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Section: Resultsmentioning

confidence: 99%

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

et al. 2019

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“…The discerned peak at 1686 cm −1 can be attributed to the stretching band of CO, which was attached to the aromatic ring of hydroxyproline and proline proteins in the collagen type I structure. The triple helical structure of collagen type I is typically characterized by the absorbance peak at 1637 cm −1 . The peaks at 1650 and 1540 cm −1 can be assigned to the amide‐type I and amide type II, which can be related to the vibration of peptide bonds (stretching modes of CO and CN, and bending mode of NH).…”

Section: Resultsmentioning

confidence: 99%

Low‐pressure plasma surface modification of polyurethane films with chitosan and collagen biomolecules

Bahrami

Khorasani

Mahdavi

et al. 2019

J of Applied Polymer Sci

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Polyurethane (PU) was synthesized using castor oil and a trade grade of hexamethylene diisocyanate, and then PU films were prepared for wound dressing applications. The PU films were then plasma treated with the low-pressure nitrogen plasma to functionalize with peroxide and hydroperoxide groups in order to attach with acrylic acid monomers. Therefore, the polyacrylic acid polymer branches were formed on the film surfaces. Carboxylic acid groups were activated by N-(3-dimethylaminopropyl)-N 0 -ethyl carbodiimide hydrochloride/N-hydroxysuccinimide and bonded with chitosan and collagen biomolecules. Untreated, nitrogen plasma treated, polyacrylic acid grafted, and finally chitosan and collagen-immobilized PU films were characterized by several tests. The tests included the attenuated total reflectance Fourier transform infrared spectroscopy, static contact angle, atomic force microscopy, scanning electron microscopy, fibroblast L929 cell culture, and antibacterial activity assay to evaluate their in vitro cytocompatibility. The results confirmed that chitosan and collagen were immobilized successfully on the PU surfaces. The chitosan-immobilized PU and collagen-immobilized PU improved the adhesion and proliferation of fibroblast cells compared to untreated PU films. The chitosanmodified PU films exhibit the best antibacterial properties.

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“…FTIR spectroscopy has been demonstrated to be an effective and non-invasive diagnostic method that characterizes the biochemical changes in tissues, cells, and even at the molecular level [ 8 ]. Oligodendroglial and astrocytic tumor cells can easily be identified by their infrared spectra, with characteristic features in the lipid acyl chain region as well as in the nucleic acid region [ 9 , 10 ]. For glioblastoma stem cells, attenuated total reflection (ATR) FTIR spectroscopy allows correct classification of the tissues, differentiating between CD133-rich and CD133-poor populations [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Biochemical Properties of Human Astrocytes, Microglia, Glioma, and Glioblastoma Cells Using Fourier Transform Infrared Spectroscopy

et al. 2020

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Background With infiltration, high-grade glioma easily causes the boundary between tumor tissue and adjacent tissue to become unclear and results in tumor recurrence at or near the resection margin according to the incomplete surgical resection. Fourier transform infrared spectroscopy (FTIR) technique has been demonstrated to be a useful tool that yields a molecular fingerprint and provides rapid, nondestructive, high-throughput and clinically relevant diagnostic information. Material/Methods FTIR was used to investigate the morphological and biochemical properties of human astrocytes (HA), microglia (HM1900), glioma cells (U87), and glioblastoma cells (BT325) cultured in vitro to simulate the infiltration area, with the use of multi-peak fitting and principal component analysis (PCA) of amide I of FTIR spectra and the use of hierarchical cluster analysis (HCA). Results We found that the secondary structures of the 4 types of cells were significantly different. The contents of α-helix structure in glial cells was significantly higher than in the glioma cells, but the levels of β-sheet, β-turn, and random coil structures were lower. The 4 types of cells could be clearly separated with 85% for PC1 and 12.2% for PC2. Conclusions FTIR can be used to distinguish between human astrocytes, microglia, glioma, and glioblastoma cells in vitro . The protein secondary structure can be used as an indicator to distinguish tumor cells from glial cells. Further tissue-based and in vivo studies are needed to determine whether FTIR can identify cerebral glioma.

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Infrared spectroscopy as a new tool for studying single living cells: Is there a niche?

Cited by 53 publications

References 74 publications

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering

Low‐pressure plasma surface modification of polyurethane films with chitosan and collagen biomolecules

Morphological and Biochemical Properties of Human Astrocytes, Microglia, Glioma, and Glioblastoma Cells Using Fourier Transform Infrared Spectroscopy

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