Infrared Microspectroscopic Imaging of Biomineralized Tissues using a Mercury-Cadmium-Telluride Focal-Plane Array Detector

Marcott, Curtis; Rc, Reeder; Ep, Paschalis; Tatakis, DN; Boskey, Adele L.; Mendelsohn, R.

doi:10.1080/10426509908039585

Cited by 26 publications

(5 citation statements)

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“…Specifically, age-and site-dependent changes in relative mineral content, as well as relative crystal maturity, are demonstrated in the whole incisors. FTIRI as an analytical imaging method has been used to obtain qualitative and quantitative information on both mineral and matrix of bone (Marcott et al, 1999;Mendelsohn et al, 1999;Boskey et al, 2002b), calcifying cartilage cultures (Boskey et al, 2002a), and cartilage (Bhargava and Levin, 2001;Camacho et al, 2001), with a spatial resolution of ~ 7 m. The two spectroscopic parameters, mineral:matrix ratio and crystallinity, were selected for dentin analysis because, as ratios, they are independent of sectioning artifacts, and they represent important properties. The mineral:matrix ratio is linearly related to the chemically measured ash weight (Pienkowski et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Spectroscopic Imaging of Mineral Maturation in Bovine Dentin

et al. 2003

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Dentin is a useful model for the study of mineral maturation. Using Fourier Transform Infrared Imaging (FTIRI), we characterized distinct regions in developing dentin at 7- micro m spatial resolution. Mineral-to-matrix ratio and crystallinity in bovine dentin from cervical and incisal parts of 3rd-trimester fetal compared with one-year-old incisor crowns showed that virtually all maturation stages in dentin could be spectroscopically isolated and analyzed. In the fetal incisors, mantle and circumpulpal dentin presented distinct patterns of mineral maturation. Gradients in both mineral properties examined were observed at the mineralization front and at the dentino-enamel junction.

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Section: Discussionmentioning

confidence: 99%

Spectroscopic Imaging of Mineral Maturation in Bovine Dentin

et al. 2003

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“…Water vapor and PMMA spectral interferences were corrected for as published elsewhere. (18) Parameters calculated were (1) mineral crystal maturity/crystallinity, expressed as absorbance ratio at two specific wavelengths (1030 and 1020 cm −1 ) (18) ; and (2) relative ratio of pyridinium and deH-DHLNL collagen cross-links, also expressed as the absorbance ratio at two specific wavelengths (1660 and 1690 cm −1 ). (20) For each section analyzed, absorbance ratios were expressed as color-coded images (using the same scale for all data; Origin 6.0, Microcal Software, Northampton, MA, USA) to depict spatial distribution of outcomes.…”

Section: Ftiri Data Acquisition and Spectral Image Analysismentioning

confidence: 99%

“…Analysis focused on trabecular bone to determine the effect of treatment on mineral maturity/crystallinity and collagen cross-links as a function of trabecular surface metabolic activity or tissue age (forming versus resorbing surfaces). FTIRI is a validated spectroscopic technique that allows the determination of mineral maturity/crystallinity (apatite stoichiometry, ionic lattice substitutions, mineral crystallite size), and the ratio of pyridinium (nonreducible) to dehydro-dihydroxylysinonorleucine (deDHLNL; reducible) collagen cross-links in mineralized sections of bone at the microscopic level, with a spatial resolution of ∼6.3 m. (18)(19)(20) It has been previously used to study differences between normal and osteoporotic bone. (21)(22)(23)(24)(25) It has been also used to examine effects of other antiresorptive (ibandronate, cyclic hormonal replacement [HRT]) and anabolic (PTH) agents on bone material properties in trabeculae devoid of resorbing surfaces.…”

Section: Introductionmentioning

confidence: 99%

Bone Material Properties in Trabecular Bone From Human Iliac Crest Biopsies After 3- and 5-Year Treatment With Risedronate

Durchschlag

Paschalis

Zoehrer

et al. 2006

Journal of Bone and Mineral Research

115

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Long-term effects of risedronate on bone mineral maturity/crystallinity and collagen cross-link ratio in triple iliac crest biopsies of osteoporotic women were evaluated. In this double-blinded study, 3-and 5-year treatment with risedronate arrested the tissue aging encountered in untreated osteoporosis and in osteoporosis treated with other antiresorptives. This effect may be contributing to risedronate's antifracture efficacy.Introduction: Risedronate is widely used in the treatment of osteoporosis. It reduces bone turnover, increases BMD, and decreases fracture risk. To date, there are no data available on the long-term effects of risedronate on bone material properties in humans. Materials and Methods: Osteoporotic women enrolled in the VERT-NA trial received either risedronate (5 mg/day, orally) or placebo for up to 5 years. All subjects received calcium. They also received vitamin D supplementation if deficient at baseline. Triple iliac crest biopsies were collected from a subset of these subjects at baseline, 3 years, and 5 years. Mineral maturity/crystallinity and collagen cross-link ratio was measured in these biopsies using Fourier transform infrared imaging. Results: Patients that received placebo exhibited increased mineral maturity/crystallinity and collagen crosslink ratio after 3 and 5 years compared with baseline values. On the contrary, patients that received risedronate retained baseline values in both bone material indices throughout. A more spatially detailed analysis revealed that this was achieved mainly through beneficial effects on active bone-forming areas. Surprisingly, patients that received risedronate achieved premenopausal values at bone-forming areas in both indices after 5 years of treatment. Conclusion: Long-term treatment with risedronate affects bone material properties (mineral maturity/ crystallinity and collagen cross-link ratio) and arrests the tissue aging apparent in untreated osteoporosis. These changes at the material level of the bone matrix may contribute to risedronate's rapid and sustained antifracture efficacy in osteoporotic patients.

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“…(53) More recently, Fourier transform infrared microspectroscopy (FTIRM) and infrared imaging (FTIRI) have demonstrated variations in both mineral content and mineral crystal size, with the most mature mineral having the largest, more perfect crystals. (54)(55)(56)(57) Since mineral content contributes to the material component of bone's biomechanical performance, (2) it is likely that crystal properties also make a contribution. FTIRM and FTIRI also reveal differences in collagen maturity throughout normal trabeculae that are not detected in osteoporotic trabeculae.…”

Section: Bone Ultrastructure and Bone Strengthmentioning

confidence: 99%