Collagen and Bone Strength

Boskey, Adele L.; Tm, Wright; Blank, Robert D.

doi:10.1359/jbmr.1999.14.3.330

Cited by 181 publications

(93 citation statements)

References 83 publications

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“…The 13we mice had significantly greater tissue-level modulus, yield stress and ultimate stress compared with 11we mice (Figure 3), properties that suggest the mineral was altered after exercise was terminated [18,20,24,26]. However, mineral composition and density were not measured in the current study, so this hypothesis cannot be specifically addressed.…”

Section: Discussionmentioning

confidence: 75%

“…Since post-yield properties of bone are primarily dictated by the organic portion of the ECM [20][21][22][23][24], and crosssectional size and shape did not change with loading, exercise likely exerted its initial effects through changes in the organic compartment of pre-existing bone. In fact, recent studies utilizing Raman microspectroscopy to probe the chemical composition of bone indicate that exercise increases the overall maturity of collagen cross-linking in the tibiae of male mice of this background strain [25].…”

Section: Discussionmentioning

confidence: 99%

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Short-Term Exercise in Mice Increases Tibial Post-Yield Mechanical Properties While Two Weeks of Latency Following Exercise Increases Tissue-Level Strength

2009

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We have previously shown that exercise during growth increases post-yield deformation in C57BL6/129 (B6;129) male tibiae at the expense of reduced pre-yield deformation and structural and tissue strength. Other research in the literature indicates that increased mineral, cross-sectional geometry and structural strength due to exercise can be maintained or increased after exercise ends for as long as 14 weeks. It was therefore hypothesized that after our exercise protocol ends, effects of exercise on mechanical properties would persist, resulting in increased post-yield behavior and rescued strength versus age-matched control mice. Beginning at 8 weeks of age, exercise consisted of running on a treadmill (30 min/day, 12 m/min, 5° incline) for 21 consecutive days. At the end of running and 2 weeks later, in the cortical bone of the tibial mid-diaphyses of B6;129 male mice, changes due to exercise and latency following exercise were assayed by mechanical tests and analyses of cross-sectional geometry. Exercise increased structural post-yield deformation compared with weight-matched control mice, without changes in bone size or shape, suggesting that exercised-induced changes in pre-existing bone quality were responsible. Over the two week latency period, no growth related changes were noted in control mice, but exercise-induced changes resulted in increased tissue stiffness and strength versus mice sacrificed immediately after exercise ended. Our data indicate that periods of exercise followed by latency can alter strength, stiffness and ductility of bone independent of changes in size or shape, suggesting that exercise may be a practical way to increase the quality of the bone extracellular matrix.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Short-Term Exercise in Mice Increases Tibial Post-Yield Mechanical Properties While Two Weeks of Latency Following Exercise Increases Tissue-Level Strength

2009

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“…Highly mineralized bone is stiffer, more brittle, and less tough than bone with less mineralization [42,49]. The increase of overall porosity in cortical bone is also responsible for a decrease of bone mechanical properties [50,51], whereas the quality of the collagen matrix was shown to predominantly determine the work to failure of bone [43,[52][53][54][55]. Further, it was reported that bone cells had different levels of activity in various inbred strains, resulting in different bone tissue properties, especially in the amount of minerals and remodeling rates [56,57].…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Bone Structural and Material Properties on Bone Competence in C57BL/6 and C3H/He Inbred Strains of Mice

2008

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The femoral neck is a relevant and sensitive site for studying the degree of osteopenia. Engineering principles predict that bone structural parameters, like cross-sectional geometry, are important determinants of bone mechanical parameters. Mechanical parameters are also directly affected by the material properties of the bone tissue. However, the relative importance of structural and material properties is still unknown. The aim of this study was to compare bone competence and structural parameters between a murine strain showing a low bone mass phenotype, C57BL/6 (B6), and another one showing a high bone mass phenotype, C3H/He (C3H), in order to better determine the role of bone structure and geometry in bone failure behavior. Murine femora of 12-and 16-week-old B6 and 12-and 16-week-old C3H inbred strains were mechanically tested under axial loading of the femoral head. In order to assess the structural properties, we performed three-dimensional morphometric analyses in five different compartments of the mouse femur using micro-computed tomography. The mechanical tests revealed that B6 femora became stiffer, stronger, and tougher at 12-16 weeks, while bone brittleness stayed constant. C3H bone stiffness increased, but strength remained constant, work to failure decreased, and bone became more brittle. These age effects indicated that B6 did not reach peak bone properties at 16 weeks of age and C3H did reach maximal skeletal biomechanical properties before 16 weeks of age. Our investigations showed that 83% of the strength of the femoral neck in the B6 strain was explained by cortical thickness at this location; in contrast, in C3H none of the mechanical properties of the femoral neck was explained by bone structural parameters. The relative contributions of bone structural and material properties on bone strength are different in B6 and C3H. We hypothesize that these different contributions are related to differences at the ultrastructural level of bone that affect bone failure.

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“…The bone extracellular matrix is composed of collagens, mostly COL I, which constitutes 90 % of the total organic extracellular matrix in mature bone (Boskey et al 1999) and noncollagenous proteins, such as BSP (Bianco et al 1991). The addition of LMW fucoidan at 0.25-2 mg/ml resulted in a significant increase in COL I mRNA level (Fig.…”

Section: Effect Of Lmw Fucoidan On Various Gene Expression Levels Of mentioning

confidence: 94%

The in vitro and in vivo effects of the low molecular weight fucoidan on the bone osteogenic differentiation properties

et al. 2015

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Osteoporosis has been reported as a hidden death factor in aged people. So far, prevention and treatment therapies for osteoporosis only slow down the progress but do not treat the disease. Fucoidan has been recognized its roles in anti-tumor, anti-inflammatory, anti-coagulant and antiviral activities. To date, low molecular weight (LMW) fucoidan role in bone loss disease has been not determined yet. Therefore, this study aims to figure out potential effects of LMW fucoidan in osteoporosis in vitro and in vivo. LMW fucoidan was extracted from fresh Sargassum hemiphyllum showing a significant increase in 7F2 cell viability to 150.33 ± 6.50 % relative to normal fucoidan (130.12 ± 5.74 %). The expression of level BMP-2, ALP, osteocalcin significantly increased with 2.28 ± 0.06, 2.18 ± 0.12 and 2.06 ± 0.07 fold, respectively. The RT-PCR assay showed that LMW fucoidan increased mRNA expression of BMP-2, ALP, osteocalcin, COL I, BSP and osteonectin. Furthermore, the bone density and bone ash weight were considerably boosted by the oral administration of280 mg/kg LMW fucoidan and 100 mg/kg calcium carbonate in C57BL/6J female aged mice. The present finding indicated that LMW fucoidan triggered osteogenic differentiation in vitro, and had an anabolic effect on bone mineralization in vivo. Dietary intake of LMW fucoidan from S. hemiphyllum suggested playing a role in the enhancement of bone loss with increasing age.

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Collagen and Bone Strength

Cited by 181 publications

References 83 publications

Short-Term Exercise in Mice Increases Tibial Post-Yield Mechanical Properties While Two Weeks of Latency Following Exercise Increases Tissue-Level Strength

Short-Term Exercise in Mice Increases Tibial Post-Yield Mechanical Properties While Two Weeks of Latency Following Exercise Increases Tissue-Level Strength

Differential Effects of Bone Structural and Material Properties on Bone Competence in C57BL/6 and C3H/He Inbred Strains of Mice

The in vitro and in vivo effects of the low molecular weight fucoidan on the bone osteogenic differentiation properties

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