The in vitro and in vivo effects of the low molecular weight fucoidan on the bone osteogenic differentiation properties

Abstract

Osteoporosis has been reported as a hidden death factor in aged people. So far, prevention and treatment therapies for osteoporosis only slow down the progress but do not treat the disease. Fucoidan has been recognized its roles in anti-tumor, anti-inflammatory, anti-coagulant and antiviral activities. To date, low molecular weight (LMW) fucoidan role in bone loss disease has been not determined yet. Therefore, this study aims to figure out potential effects of LMW fucoidan in osteoporosis in vitro and in vivo. LMW fucoidan was extracted from fresh Sargassum hemiphyllum showing a significant increase in 7F2 cell viability to 150.33 ± 6.50 % relative to normal fucoidan (130.12 ± 5.74 %). The expression of level BMP-2, ALP, osteocalcin significantly increased with 2.28 ± 0.06, 2.18 ± 0.12 and 2.06 ± 0.07 fold, respectively. The RT-PCR assay showed that LMW fucoidan increased mRNA expression of BMP-2, ALP, osteocalcin, COL I, BSP and osteonectin. Furthermore, the bone density and bone ash weight were considerably boosted by the oral administration of280 mg/kg LMW fucoidan and 100 mg/kg calcium carbonate in C57BL/6J female aged mice. The present finding indicated that LMW fucoidan triggered osteogenic differentiation in vitro, and had an anabolic effect on bone mineralization in vivo. Dietary intake of LMW fucoidan from S. hemiphyllum suggested playing a role in the enhancement of bone loss with increasing age.

Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

“…This result is consistent with our earlier findings, in which the ≤6 kDa fucoidan shows the highest antibacterial activity [6]. It is believed that sulfated polysaccharides (such as fucoidans) with low molecular weight can easily penetrate cell membranes [19,20]. Thus, molecular weight should be one of critical factors determining the antibacterial activity of sulfated polysaccharides.…”

Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway

“…However, the dose of 100 mg/kg/d did not improve renal function and even slightly worsened renal histologic change in CKD mice. In animal studies, low-molecular weight fucoidan doses of 100 mg/kg/d or higher are often applied to studies of diseases such as cancers, diabetic retinopathy, renal acute injury, and osteoporosis, and exerts significant protective effects25262728. Our results revealed that the high oligo-fucoidan dose promoted renal function and increased the creatinine clearance rate (Fig.…”

Fucoidan‐induced osteogenic differentiation promotes angiogenesis by inducing vascular endothelial growth factor secretion and accelerates bone repair

“…A recent review studying in vivo bone repair experiments showed that low molecular weight fucoidan obtained from brown seaweed increases bone density and bone ash weight following oral administration in mice. These effects were thought to be directly triggered by the osteogenic effect of fucoidan (Hwang et al, ). For example, Pereira et al () showed that treatment of implanted human adipose‐derived stem cells with fucoidan obtained from brown seaweed significantly increased new bone formation in a mouse model.…”