Infrared‐assisted extraction coupled with high‐performance liquid chromatography for simultaneous determination of eight active compounds in Radix Salviae miltiorrhizae

Abstract

In this study, a novel infrared-assisted extraction (IRAE) method, in which infrared radiation was employed to extract the active compounds from plant, was developed and coupled with HPLC for simultaneous determination of four phenolic acids and four diterpenoids in Radix Salviae miltiorrhizae (Chinese name Danshen). The extraction conditions of IRAE were optimized and the optimal conditions were as follows: extraction time of 15 min; extraction solvent of 70% v/v methanol in water solution, and solid/liquid ratio of 0.1:15 (g/mL). Chromatography was performed on a 200 mm×4.6 mm id, 5-μm particle size, C18 column. Good linearity (r>0.9994) was observed over the concentration ranges investigated, and intra-day and inter-day precision were high. Recoveries of the eight compounds were from 96.90 to 104.30% and RSD was below 2.5%. By using this novel IRAE method, with a shorter extraction time, the determined amounts of the eight active components in Danshen were comparable with or even higher than those extracted with conventional heat-reflux extraction and ultrasound-assisted extraction methods and microwave-assisted extraction. The developed IRAE method is simple, low-cost and efficient, offering a great promise for quick determination of active compounds in natural plants.

Study on infrared-assisted extraction coupled with high performance liquid chromatography (HPLC) for determination of catechin, epicatechin, and procyanidin B2 in grape seeds

“…Like microwaveassisted extraction (MAE), the ability to rapidly heat the sample solvent mixture is inherent to IRAE. By using closed vessels, the extraction can be performed at elevated temperatures accelerating the mass transfer of target compounds from the sample matrix (Chen, Duan, Xie, & Li, 2010). In my study, the temperature of the IRAE was measured, and the mixed solvent reached the boiling point (79°C) within 7 min, while with the classical electrical heating method the temperature increased slower and reached the boiling point (79°C) at 13 min.…”

A C₈-Modified Graphene@mSiO₂ Composites Based Method for Quantification of Gallic Acid in Rat Plasma after Oral Administration of Changtai Granule and Its Application to Pharmacokinetics

“…To date, several studies on pharmacokinetics of GA in its pure form have been reported. 4,[6][7][8] However, to the best of our knowledge, no study on pharmacokinetic behaviors of GA after oral administration of Changtai Granule has ever been reported.…”

Metabolomic profiles of myocardial ischemia under treatment with salvianolic acid B

“…Thus, most scientists hope to increase understanding of mechanisms of Danshen by analyzing active components of Danshen . Presently, many active components have been isolated and identified in Danshen , such as tanshinone IIA, and salvianolic acid B (SA-B) [12-14]. …”