Infradian Rhythm in Proliferative Activity of a Culture of Embryonic Fibroblast-Like Cells from C57BL/6 Mice

Диатроптова, М. А.; Ponomarenko, E A; Diatroptov, M. E.

doi:10.1007/s10517-020-04962-y

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Based on this hypothesis, the 4-day rhythm of mitotic activity in an isolated cell culture should be absent. However, studying the daily dynamics of the proliferative activity of the culture of embryonic fibroblast-like cells in the logarithmic growth phase, we revealed a 4-day rhythm that persists for at least three weeks of cultivation 15 .…”

Section: Introductionmentioning

confidence: 93%

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

Диатроптова

Косырева

Diatroptov

2022

Sci Rep

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A study of the 4-day rhythm of the proliferative activity of the embryonic fibroblast-like cells in the logarithmic growth phase was carried out. It was shown that in cell cultures obtained on different days from embryos of different ages, the phase of the 4-day rhythm coincides. In vitro the maxima of the proliferative activity were consistent with the minima of the motor activity of mice. Freezing the culture for 2 or 6 days does not cause a shift in the phase of the 4-day rhythm of cell proliferative activity compare with the unfreezing culture. That indicates the existence of an external synchronizer, which determines the 4-day infradian rhythm of the proliferative activity of embryonic cells. Then we daily thawed samples of single L929 culture of mice fibroblast-like cells for 22 and 17 days and researched the dynamics of its proliferative activity. We also showed 4-day rhythm of the simultaneous increase in the number of cells for all thawed samples. Taking into account that deep freezing of a culture leads to the cessation of all life processes, the fact we obtained indicates an exogenous mechanism of the formation of about a 4-day rhythm of the proliferative activity of cell culture. Variations of the Earth's magnetic field could be one of the external synchronizers of the infradian rhythm. We studied the increase in number of L929 cell in conditions of a magnetic permalloy screen and showed that the magnetic shielding no affect the parameters of the infradian rhythm of L929 cell proliferative activity. So further searches of the external synchronizers are need.

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Section: Introductionmentioning

confidence: 93%

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

Диатроптова

Косырева

Diatroptov

2022

Sci Rep

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“…However, studying the daily dynamics of the proliferative activity of the culture of embryonic broblastlike cells in the logarithmic growth phase, we revealed a 4-day rhythm that persists for at least three weeks of cultivation [15].…”

Section: Introductionmentioning

confidence: 94%

About 4-day Rhythm of Proliferative Activity of Fibroblast-like Cell Cultures Depends on the Environmental Factor

Диатроптова

Косырева

Diatroptov

2021

Preprint

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A study of the 4-day rhythm of the proliferative activity of the embryonic fibroblast-like cells in the logarithmic growth phase was carried out. It was shown that in cell cultures obtained on different days from embryos of different ages, the phase of the 4-day rhythm coincides. In vitro the maxima of the proliferative activity were consistent with the minima of the motor activity of mice. Freezing the culture for 2 or 6 days does not cause a shift in the phase of the 4-day rhythm of cell proliferative activity compare with the unfreezing culture. That indicates the existence of an external synchronizer, which determines the 4-day infradian rhythm of the proliferative activity of embryonic cells. Then we daily thawed samples of single L-929 culture of mice fibroblast-like cells for 22 and 17 days and researched the dynamics of its proliferative activity. We also showed 4-day rhythm of the simultaneous increase in the number of cells for all thawed samples. Taking into account that deep freezing of a culture leads to the cessation of all life processes, the fact we obtained indicates an exogenous mechanism of the formation of about a 4-day rhythm of the proliferative activity of cell culture.

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Infradian 4-Day Rhythm of Proliferative Activity of L-929 Mouse Fibroblast Culture in the Logarithmic Growth Phase Is Exogenous

et al. 2021

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Infradian Rhythm in Proliferative Activity of a Culture of Embryonic Fibroblast-Like Cells from C57BL/6 Mice

Cited by 3 publications

References 10 publications

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

About 4-day Rhythm of Proliferative Activity of Fibroblast-like Cell Cultures Depends on the Environmental Factor

Infradian 4-Day Rhythm of Proliferative Activity of L-929 Mouse Fibroblast Culture in the Logarithmic Growth Phase Is Exogenous

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