Infradian 4-Day Rhythm of Proliferative Activity of L-929 Mouse Fibroblast Culture in the Logarithmic Growth Phase Is Exogenous

Диатроптова, М. А.; Косырева, А. М.; Makarova, Olga; Diatroptov, M. E.

doi:10.1007/s10517-021-05364-4

Cited by 4 publications

(1 citation statement)

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“…Therefore, at the final stage of our research, we studied the changes in the proliferative activity of the fiboroblast-like cells mice culture L929, which has a high proliferative potential. According to our latest data a proliferative activity of L929 cells have the about 4-day infradian rhythm too 16 . The aim of the 4th point of the study was: to establish the exogenous/endogenous nature of about 4 days of the rhythm of proliferative activity of fibroblast-like L929 cells by daily thawing of same frozen samples of the same culture.…”

Section: Introductionmentioning

confidence: 93%

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

Диатроптова

Косырева

Diatroptov

2022

Sci Rep

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A study of the 4-day rhythm of the proliferative activity of the embryonic fibroblast-like cells in the logarithmic growth phase was carried out. It was shown that in cell cultures obtained on different days from embryos of different ages, the phase of the 4-day rhythm coincides. In vitro the maxima of the proliferative activity were consistent with the minima of the motor activity of mice. Freezing the culture for 2 or 6 days does not cause a shift in the phase of the 4-day rhythm of cell proliferative activity compare with the unfreezing culture. That indicates the existence of an external synchronizer, which determines the 4-day infradian rhythm of the proliferative activity of embryonic cells. Then we daily thawed samples of single L929 culture of mice fibroblast-like cells for 22 and 17 days and researched the dynamics of its proliferative activity. We also showed 4-day rhythm of the simultaneous increase in the number of cells for all thawed samples. Taking into account that deep freezing of a culture leads to the cessation of all life processes, the fact we obtained indicates an exogenous mechanism of the formation of about a 4-day rhythm of the proliferative activity of cell culture. Variations of the Earth's magnetic field could be one of the external synchronizers of the infradian rhythm. We studied the increase in number of L929 cell in conditions of a magnetic permalloy screen and showed that the magnetic shielding no affect the parameters of the infradian rhythm of L929 cell proliferative activity. So further searches of the external synchronizers are need.

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Section: Introductionmentioning

confidence: 93%