Influenza Viruses with Rearranged Genomes as Live-Attenuated Vaccines

Pena, Lindomar; Sutton, Troy; Chockalingam, Ashok; Kumar, Sachin; Angel, Matthew; Shao, Hongxia; Chen, Hongjun; Li, Weizhong; Pérez, Daniel R.

doi:10.1128/jvi.02490-12

Cited by 62 publications

(95 citation statements)

References 30 publications

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“…Considering naturally selected lack of virulence of waterfowl-origin LPAIV, many researchers tried to use the genes of these viruses for the development of live influenza vaccines (Murphy et al, 1982, Crawford et al, 1998, Van Der Coot et al, 2003Shi et al, 2007;Wu et al, 2010;Zhang et al, 2012;Pena et al, 2013). Our data confirm that the H5N3 wild duck virus represents a promising live candidate vaccine for protection of poultry from H5N1 HPAI viruses.…”

Section: Discussionsupporting

confidence: 63%

Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus

As¹,

Ey²,

Nf³

et al. 2016

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Section: Discussionsupporting

confidence: 63%

Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus

As¹,

Ey²,

Nf³

et al. 2016

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“…In the present work, I confirmed that 2A CHYSEL activity (20)(21)(22)(23) is efficient in chicken cells and provides a useful tool to allow the coexpression of multiple proteins from a single promoter. This result highlights the potential of MDV as versatile avian multigene delivery vector.…”

Section: Fig 3 (A)supporting

confidence: 80%

“…In addition, tagging of viral proteins, either at the C or N terminus, is likely to be detrimental for virus replication (12,16). To overcome this issue, I used the cis-acting hydrolase element (CHYSEL) (20)(21)(22)(23). This strategy is based on the insertion of the 2A ribosomal skipping site, which provides an efficient way to express comparable levels and physically separate proteins from a single promoter.…”

mentioning

confidence: 99%

A Virulent Bioluminescent and Fluorescent Dual-Reporter Marek's Disease Virus Unveils an Alternative Spreading Pathway in Addition to Cell-to-Cell Contact

Harmache

2014

J Virol

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Marek's disease virus (MDV) is a growing threat for the poultry industry. Unfortunately, despite successful vaccination against the disease, MDV remains in circulation within vaccinated flocks, leading to the selection of increasingly virulent pathotypes. Detailed knowledge of the virus biology and the host-virus interaction is required to improve the vaccine efficiency. In the present study, I engineered an original, dual-reporter MDV to track and quantify virus replication in vitro and in vivo.

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“…In addition, Gao et al reported the generation of a nine-segment virus containing two HAs, one of which was incorporated by replacing the ORF of PB2 with the H3 HA ORF (35). Very recently, Pena et al generated a recombinant influenza virus by rearranging the genome of an avian H9N2 virus and expressed the entire H5 HA ORF from the NS segment RNA (54). All these results suggest the possibility and feasibility of rescuing influenza A viruses by replacing the coding region of HA, NA, or other influenza virus Previous studies demonstrated that an elastase-dependent SIV could be used as a LAIV against homologous, homologous heterotypic, and heterologous SIV isolates (11,14).…”

Section: Discussionmentioning

confidence: 99%

An Eight-Segment Swine Influenza Virus Harboring H1 and H3 Hemagglutinins Is Attenuated and Protective against H1N1 and H3N2 Subtypes in Pigs

Mašić

Pyo

Babiuk

et al. 2013

J Virol

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Swine influenza virus (SIV) infections continue to cause production losses in the agricultural industry in addition to being a human public health concern. The primary method of controlling SIV is through vaccination. The killed SIV vaccines currently in use must be closely matched to the challenge virus, and their protective efficacy is limited. Live attenuated influenza vaccines (LAIV) provide strong, long-lived cell-mediated and humoral immunity against different influenza virus subtypes with no need for antigen matching. Here we report the generation of a new potential LAIV, an eight-segment SIV harboring two different SIV hemagglutinins (HAs), H1 and H3, in the genetic background of H1N1 SIV. This mutant SIV was generated by fusing the H3 HA ectodomain from A/Swine/Texas/4199-2/98 (H3N2) to the cytoplasmic tail, transmembrane domain, and stalk region of neuraminidase (NA) from A/Swine/Saskatchewan/18789/02 (H1N1) SIV. While this H1-H3 chimeric SIV, when propagated in vitro in the presence of exogenous neuraminidase, showed kinetics and growth properties similar to those of the parental wild-type virus, in vivo it was highly attenuated in pigs, demonstrating a great potential for serving as a dual LAIV. Furthermore, vaccination with the H1-H3 virus elicited robust immune responses, which conferred complete protection against infections with both H1 and H3 SIV subtypes in pigs.

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Influenza Viruses with Rearranged Genomes as Live-Attenuated Vaccines

Cited by 62 publications

References 30 publications

Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus

Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus

A Virulent Bioluminescent and Fluorescent Dual-Reporter Marek's Disease Virus Unveils an Alternative Spreading Pathway in Addition to Cell-to-Cell Contact

An Eight-Segment Swine Influenza Virus Harboring H1 and H3 Hemagglutinins Is Attenuated and Protective against H1N1 and H3N2 Subtypes in Pigs

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