Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus

As, Gambaryan; Ey, Boravleva; Nf, Lomakina; Ea, Kropotkina; Gordeychuk, Ilya; Chvala, Ilya; Vv, Drygin; Hd, Klenk; Matrosovich, Mikhail

doi:10.4149/av_2016_03_316

Cited by 8 publications

(16 citation statements)

References 37 publications

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“…Три вируса испытали на цыплятах и также выявили их апатогенность [17]. Геном нескольких вирусов частично или полностью секвенировали [18]. Все выделенные вирусы проана-лизированы на микрочипе «Биогрипп».…”

Section: результатыunclassified

Применение Микрочипов Для Идентификации Происхождения Генов Вирусов Гриппа Диких Птиц

Гейдаров¹,

Nf²,

Боравлева³

et al. 2017

Microbiology Independent Research Journal (MIR Journal)

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В черте города Москвы из фекалий диких водоплавающих птиц изолировали 42 штамма вируса гриппа птиц и проанализировали их на микрочипах «Биогрипп», которые содержат 176 зондов к различным участкам генома вирусов гриппа. Микрочип позволяет определять: 1) субтип поверхностных белков гемагглютинина и нейраминидазы; 2) структуру С-концевой последовательности вирусного белка NS1, влияющую на степень ингибирования транскрипции клеточных хозяйских генов, в том числе ответственных за синтез интерферона; 3) наличие стоп-кодонов и мутацию N66S в рамке считывания вирусного белка PB1-F2, 4) наличие полиосновного сайта протеолитического расщепления гемагглютинина. Среди изолятов от диких птиц идентифицированы вирусы гриппа субтипов H3N1, H3N6, H3N8, H4N6, H1N1, H5N3 и H11N9. Все они содержали последовательность ESEV на С-конце белка NS1, полноразмерную рамку считывания для белка PB1-F2. Замена N66S в PB1-F2 обнаружена у шести штаммов. Однако такие маркеры патогенности, как последовательность ESEV (лиганд PDZ-домена) в вирусном белке NS1 и замена N66S PB1-F2 в контексте генома вирусов гриппа диких уток, не делали вирус патогенным для мышей. Все изоляты были высокоурожайны в куриных эмбрионах, инфекционны и иммуногенны для мышей, но не вызывали у этих животных клинических симптомов заболевания.

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Section: результатыunclassified

Применение Микрочипов Для Идентификации Происхождения Генов Вирусов Гриппа Диких Птиц

Гейдаров¹,

Nf²,

Боравлева³

et al. 2017

Microbiology Independent Research Journal (MIR Journal)

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“…Single immunization of 30-day-old chickens with 10 6 TCID 50 (tissue culture infective dose 50) of dk/4182 virus via drinking water completely protected chickens from the lethal dose (100 LD 50 ) of the HPAIV of H5N1 subtype [56]. The antibody response to immunization with lower doses varied between the birds and was considered unsatisfactory.…”

Section: The Impact Of the Vaccine Dose And Age Of Chickens On The Vamentioning

confidence: 99%

“…Since the administration of dk/4182 H5N3 to chi ckens and ducks via drinking water ensured the complete protection of birds from lethal viral challenges and prevented the transmission of the challenge virus, one can conclude that the dk/4182 H5N3 virus represents a promising candidate for the development of a live vaccine for the protection of poultry from H5N1 HPAI viruses [56,57].…”

Section: Perspectives Of Use Of Live Poultry Vaccines Against H5n1 Hpaivmentioning

confidence: 99%

Live poultry vaccines against highly pathogenic avian influenza viruses

Boravleva

Gambaryan

2018

Microbiology Independent Research Journal (MIR Journal)

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The widespread circulation of highly pathogenic avian influenza viruses (HPAIVs) and their occasional transmission to humans creates a constant pandemic threat and leads to significant economic losses in the poultry industry. The development of an effective and safe vaccine for the broad protection of poultry from H5N1 HPAIVs remains an important goal. Prevention of the virus transmission between ducks and chickens is important for the efficient control of the spread of avian influenza. The oral administration of live vaccines corresponds to the natural route of infection that leads to virus replication in the intestinal epithelial cells that cause a well-balanced and broad immune response providing protection against the viruses of distant clades. The broad protection is the important advantage of live-attenuated influenza vaccines when compared to inactivated ones. Here, we give an overview of the latest approaches and results in the development of live poultry vaccine candidates against HPAIVs.

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“…The best results were obtained with the dk/4182 virus, which caused no mortality or visible signs of the disease but stimulated the development of high antibody levels and offered 100% protection against subsequent lethal challenge. Our previous studies suggest that this H5N3 wild duck virus represents a promising candidate vaccine for protection of poultry from H5N1 HPAI viruses [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Immunization of Domestic Ducks with Live Nonpathogenic H5N3 Influenza Virus Prevents Shedding and Transmission of Highly Pathogenic H5N1 Virus to Chickens

Gambaryan

Gordeychuk

Boravleva

et al. 2018

Viruses

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Wild ducks are known to be able to carry avian influenza viruses over long distances and infect domestic ducks, which in their turn infect domestic chickens. Therefore, prevention of virus transmission between ducks and chickens is important to control the spread of avian influenza. Here we used a low pathogenic wild aquatic bird virus A/duck/Moscow/4182/2010 (H5N3) for prevention of highly pathogenic avian influenza virus (HPAIV) transmission between ducks and chickens. We first confirmed that the ducks orally infected with H5N1 HPAIV A/chicken/Kurgan/3/2005 excreted the virus in feces. All chickens that were in contact with the infected ducks became sick, excreted the virus, and died. However, the ducks orally inoculated with 104 50% tissue culture infective doses of A/duck/Moscow/4182/2010 and challenged 14 to 90 days later with H5N1 HPAIV did not excrete the challenge virus. All contact chickens survived and did not excrete the virus. Our results suggest that low pathogenic virus of wild aquatic birds can be used for prevention of transmission of H5N1 viruses between ducks and chickens.

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Immunization with live nonpathogenic H5N3 duck influenza virus protects chickens against highly pathogenic H5N1 virus

Cited by 8 publications

References 37 publications

Применение Микрочипов Для Идентификации Происхождения Генов Вирусов Гриппа Диких Птиц

Применение Микрочипов Для Идентификации Происхождения Генов Вирусов Гриппа Диких Птиц

Live poultry vaccines against highly pathogenic avian influenza viruses

Immunization of Domestic Ducks with Live Nonpathogenic H5N3 Influenza Virus Prevents Shedding and Transmission of Highly Pathogenic H5N1 Virus to Chickens

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