Influenza virus titrations and the inhibitor of hemagglutination in normal allantoic fluid

Abstract

Evidence is presented that aggregates of influenza virus particles can occur in suspensions of the virus in allantoic fluid. The hemagglutination inhibitor particles of normal allantoic fluid are indicated as the cause. The aggregates can be dissociated by a treatment at pI-I 10.5. I-Iemagglutinin and infectivity were reasonably stable under the circumstances under investigation. The influence of inhibitor particles on infectivity assay of influenza virus in chicken eggs is discussed.

Quantifying relative within-host replication fitness in influenza virus competition experiments

“…This value is also consistent with other in vitro measurements (Horsfall, 1954;Paucker and Henle, 1955;Gaush and Smith, 1968;Orthel, 1972;Beauchemin et al, 2008;Schulze-Horsel et al, 2009;. ξ…”

“…The rate of degradation of infectious to noninfectious virus is fixed to d inf ¼ 3:12 d À 1 ; a value estimated previously in vitro for two H1N1pdm09 viruses (Pinilla et al, 2012). This value is also consistent with other in vitro measurements (Horsfall, 1954;Paucker and Henle, 1955;Gaush and Smith, 1968;Orthel, 1972;Beauchemin et al, 2008;Schulze-Horsel et al, 2009;. For all fitted parameters in the coinfection model, a range of biologically plausible values are specified (Table 2) which restrict the parameter space searchable by the GA.…”

Inhaled biguanides and mTOR inhibition for influenza and coronavirus (Review)

“…They determined, with the method of surviving allantoic sections, that the minimal infectious dilution of influenza virus causing hemagglutination was 10 -5 (Table I). Allantoic sections contain an inhibitor of hemagglutination, and this method is a standard assay of viral infectivity (22).…”