Influenza A (H₁N₁) Antiviral and Cytotoxic Agents from Ferula assa-foetida

Abstract

Two new sesquiterpene coumarins, designated 5'-acetoxy-8'-hydroxyumbelliprenin (1) and 10'R-acetoxy-11'-hydroxyumbelliprenin (2), and a new diterpene, 15-hydroxy-6-en-dehydroabietic acid (3), along with 27 known compounds, were isolated from a CHCl(3)-soluble extract of Ferula assa-foetida through bioassay-guided fractionation. The structures of the new metabolites 1-3 were identified by spectroscopic data interpretation and by the Mosher ester method. Compounds 4 and 6-13 showed greater potency against influenza A virus (H(1)N(1)) (IC(50) 0.26-0.86 microg/mL) than amantadine (IC(50) 0.92 microg/mL), and 11 exhibited the best potency (IC(50) 0.51, 2.6, and 3.4 microg/mL) of these compounds against the HepG2, Hep3B, and MCF-7 cancer cell lines, respectively.

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“…The mentioned study further streamlined for use of this gum resin in the upper respiratory diseases in traditional medicine. [7] Antifungal Activity The oils extracted from all seeds except mustard showed a variation of the degree of fungicidal activity against experimental species. Asafoetida oil considerably inhibited the growth of all test fungi except.…”

“…c. 10-R-acetoxy-11 hydroxyumbelliprenin has antiviral (influenza H1N1) activity. [7] d. Epi-conferdione has antiviral (influenza H1N1). [7] e. Conferol has antiviral (influenza H1N1), cytotoxic (HepG2, Hep3B, and MCF-7), synergistic effect with anticancer agents (vincristine).…”

“…The fractionation studies of Asafoetida have led to the identification of some exciting bioactive compounds like antiviral sesquiterpene coumarins obtained from Asafetida which is more potent than amantadine against influenza-A. [7] Asafoetida is a bitter taste and possess characteristic sulfurous odor. In addition, people in Nepal eat it, in daily diets and also thought that Asafoetida has diuretic, sedative, and aphrodisiac properties.…”

“…[8,9] It is also used in the treatment of various diseases such as intestinal parasites, flatulence, influenza, epilepsy, stomachache, asthma, and weak digestion. [3,5,7] The pharmacological studies include antioxidant, [10] cancino preventive, [11,12] antidiabetic, [13] antiviral, [7] hypotensive, [14] antifungal, [15][16][17] antispasmodic, [14] and molluscicidal [17,18] from this oleo-gum-resin. It is used to treat worm infections as well as snake and insect bites.…”

In VITRO ANTIDIABETIC EFFECTS OF FERULA ASSA-FOETIDA EXTRACTS THROUGH DIPEPTIDYL PEPTIDASE IV AND Α-Glucosidase INHIBITORY ACTIVITY

“…Traditionally, it is used for the treatment of different diseases, such as stomach ache, asthma, epilepsy, flatulence, weak digestion, intestinal parasites and influenza [12]. Recent pharmacological and biological studies have also shown several activities, such as antioxidant [13], antiviral [14], antimicrobial [15], antispasmodic and hypotensive [16], and antidiabetic [5,17].…”