Influencing chondrogenic differentiation of human mesenchymal stromal cells in scaffolds displaying a structural gradient in pore size

Luca, Andrea Di; Szlązak, Karol; Lorenzo-Moldero, Ivan; Ghebes, Corina Adriana; Lepedda, Antonio Junior; Święszkowski, Wojciech; Blitterswijk, Clemens van; Moroni, Lorenzo

doi:10.1016/j.actbio.2016.03.014

Cited by 97 publications

(72 citation statements)

References 47 publications

(50 reference statements)

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“…One group has recently demonstrated that gradient pore sizes created by FDM can slightly improve both chondrogenesis 49 and osteogenesis 48 , although they did not investigate different geometries. By designing scaffold pore sizes and geometries based on biological mechanical requirements, these improvements may be further enhanced.…”

Section: 3d Printing For Craniofacial Bone Regenerationmentioning

confidence: 99%

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

et al. 2016

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The treatment of craniofacial defects can present many challenges due to the variety of tissue-specific requirements and the complexity of anatomical structures in that region. 3D-printing technologies provide clinicians, engineers and scientists with the ability to create patient-specific solutions for craniofacial defects. Currently, there are 3 key strategies that utilize these technologies to restore both appearance and function to patients: rehabilitation, reconstruction and regeneration. In rehabilitation, 3D-printing can be used to create prostheses to replace or cover damaged tissues. Reconstruction, through plastic surgery, can also leverage 3D-printing technologies to create custom cutting guides, fixation devices, practice models and implanted medical devices to improve patient outcomes. Regeneration of tissue attempts to replace defects with biological materials. 3D-printing can be used to create either scaffolds or living, cellular constructs to signal tissue-forming cells to regenerate defect regions. By integrating these three approaches, 3D-printing technologies afford the opportunity to develop personalized treatment plans and design-driven manufacturing solutions to improve aesthetic and functional outcomes for patients with craniofacial defects.

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Section: 3d Printing For Craniofacial Bone Regenerationmentioning

confidence: 99%

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

et al. 2016

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“…Different pore size gradients revealed stimulation of different cell behaviors. In this sense, the authors showed the capability to tune the scaffolds' architecture using FDM to achieve an improved induction of mesenchymal stem cells chondrogenesis and osteogenesis [34,35].…”

Section: Fused Deposition Modelingmentioning

confidence: 99%

Current advances in solid free-form techniques for osteochondral tissue engineering

et al. 2018

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Osteochondral (OC) lesions are characterized by defects in two different zones, the cartilage region and subchondral bone region. These lesions are frequently associated with mechanical instability, as well as osteoarthritic degenerative changes in the knee. The lack of spontaneous healing and the drawbacks of the current treatments have increased the attention from the scientific community to this issue. Different tissue engineering approaches have been attempted using different polymers and different scaffolds' processing. However, the current conventional techniques do not allow the full control over scaffold fabrication, and in this type of approaches, the tuning ability is the key to success in tissue regeneration. In this sense, the researchers have placed their efforts in the development of solid free-form (SFF) techniques. These techniques allow tuning different properties at the micro-macro scale, creating scaffolds with appropriate features for OC tissue engineering. In this review, it is discussed the current SFF techniques used in OC tissue engineering and presented their promising results and current challenges.

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“…The goal was to enhance cell seeding efficiency and control the spatial organization of cells within the scaffold. Some authors [22] also emphasized the importance of scaffold pore size gradients in osteogenic differentiation of human mesenchymal stromal cells. In 2010, Puppi et al [23] in deep reviewed the design of biodegradable and bioactive polymeric scaffolds, with properly suited architecture and tailored properties for bone and cartilage tissue regeneration.…”

Section: Scaffold Designmentioning

confidence: 99%

Tailoring Bioengineered Scaffolds for Regenerative Medicine

Amado¹,

Morouço²,

Pascoal‐Faria³

et al. 2018

Biomaterials in Regenerative Medicine

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The vision to unravel and develop biological healing mechanisms based on evolving molecular and cellular technologies has led to a worldwide scientific endeavor to establish regenerative medicine. This is a multidisciplinary field that involves basic and preclinical research and development on the repair, replacement, and regrowth or regeneration of cells, tissues, or organs in both diseases (congenital or acquired) and traumas. A total of over 63,000 patients were officially placed on organs' waiting lists on 31 December 2013 in the European Union (European Commission, 2014). Tissue engineering and regenerative medicine have emerged as promising fields to achieve proper solutions for these concerns. However, we are far from having patient-specific tissue engineering scaffolds that mimic the native tissue regarding both structure and function. The proposed chapter is a qualitative review over the biomaterials, processes, and scaffold designs for tailored bioprinting. Relevant literature on bioengineered scaffolds for regenerative medicine will be updated. It is well known that mechanical properties play significant effects on biologic behavior which highlight the importance of an extensively discussion on tailoring biomechanical properties for bioengineered scaffolds. The following topics will be discussed: scaffold design, biomaterials and scaffolds bioactivity, biofabrication processes, scaffolds biodegradability, and cell viability. Moreover, new insights will be pointed out.

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Influencing chondrogenic differentiation of human mesenchymal stromal cells in scaffolds displaying a structural gradient in pore size

Cited by 97 publications

References 47 publications

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

3D-Printing Technologies for Craniofacial Rehabilitation, Reconstruction, and Regeneration

Current advances in solid free-form techniques for osteochondral tissue engineering

Tailoring Bioengineered Scaffolds for Regenerative Medicine

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