Coffee production has decreased due to environmental and management factors. The current plantations are old and unproductive, also due to the rust problem, caused by the fungus Hemileia vastatrix Berk & Br. Furthermore, the shade in the production systems has decreased with the consequent increase in soil erosion, in addition to the increase in agrochemicals. Currently, the planting of new varieties with resistance to the fungus is increasing. Furthermore, it has been shown that various biofertilized perennial crops in nursery favor their growth. In this study, the effect of applying two beneficial microorganisms, Rhizophagus intraradices and/or Azospirillum brasilense, to the planting of four varieties of Coffea arabica L. was evaluated. The coffee varieties marseillase, geisha, sarchimor and costa rica 95 were established in bags with the following treatments: 1) control, 2) R. intraradices, 3) A. brasilense, 4