Influences of mechanical barrier permeability on guided bone augmentation in the rat calvarium

Yamamoto, Takanobu; Hasuike, Akira; Koshi, Ryosuke; Ozawa, Yoshihito; Ozaki, Manami; Kubota, Tatsuya; Sato, Shuichi

doi:10.2334/josnusd.17-0362

Cited by 5 publications

(14 citation statements)

References 16 publications

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“…Our primary outcome was percentage of newly formed bone in histology. The sample size was calculated with G*Power software v. 3.1(University of Dusseldorf, Dusseldorf, Germany) using Wilcoxon-Mann-Whitney test at an alpha level of 0.05 and a statistical power of 95% based on the previous study (6), in which percentage of newly formed bone from occlusive and non-occlusive barriers showed significant statistical difference between 25±3% and 17±3%. All animals were housed in cages to ensure standardized environmental factors during the experimental period.…”

Section: Methodsmentioning

confidence: 99%

“…Surgical procedures. The standardized rat calvarial vertical GBR model developed by the present group was used (3)(4)(5)(6). Before the surgical session, the animals were anesthetized via initial inhalation of 4% (v/v) isoflurane for 2 minutes, followed by intraperitoneal injection of a mixture of 0.15 mg/kg dexmedetomidine hydrochloride, 2.0 mg/kg midazolam, and 2.5 mg/kg butorphanol tartrate.…”

Section: A Circular Groove Was Made With a Trephine Bur And Five Small Holes Were Drilled With A Round Bur To Allow For Marrow Penetratiomentioning

confidence: 99%

“…To explore these biological events, it is crucial to conduct in vivo assessments of GBR using preclinical models. A well-standardized rat GBR model with plastic cylinders placed on calvarial defects was developed by our group (3)(4)(5)(6).…”

mentioning

confidence: 99%

“…Since titanium mesh is far from occlusive, there is a possibility that fibrous tissue could intrude into the bone defect area and jeopardize bone regeneration. The influence of macroporosity remains controversial (6,14,15). Several researchers have suggested that the pores of a titanium mesh play an essential role in establishing the supplement of osteogenic cells, growth factors, and blood supply from the periosteal overlying the barrier controversial (6,14).…”

mentioning

confidence: 99%

“…The influence of macroporosity remains controversial (6,14,15). Several researchers have suggested that the pores of a titanium mesh play an essential role in establishing the supplement of osteogenic cells, growth factors, and blood supply from the periosteal overlying the barrier controversial (6,14).…”

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confidence: 99%

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Comparison of Macro-and Micro-porosity of a Titanium Mesh for Guided Bone Regeneration: AnIn VivoExperimental Study

Senoo

Hasuike

Yamamoto

et al. 2021

In Vivo

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Background/Aim: Guided bone regeneration (GBR) is one of the surgical methods used for vertical ridge augmentation prior to dental implant placements. Titanium meshes have been used for osteogenic space maintenance in GBR sites by clinicians. We aimed to compare the influence of micropores and macropores in a titanium mesh on bone regeneration in a rat calvarial vertical GBR model. Materials and Methods: The calvaria of nine rats were exposed, and plastic cylinders were set bilaterally. Eighteen surgical sites were randomly allocated into three groups according to the materials of titanium lid and bone substitutes: microporous titanium lid+deproteinized bovine bone mineral (DBBM), macroporous titanium lid +DBBM, microporous titanium lid+carbonate apatite. Newly generated bone inside the cylinders was evaluated using micro-computed tomography (micro-CT). Furthermore, bone regeneration and angiogenesis were evaluated histologically at 12 weeks. Results: Quantitative volumetric analyses using micro-CT showed a gradual increase in bone volume inside the cylinders in all three groups. Histological observation confirmed vigorous bone regeneration in the microporous groups compared to that in the macroporous group. In the upper part of the cylinders, soft tissue invaded the GBR site by passing through the pores of the macroporous mesh. The blood vessels in the upper part of the cylinders were smaller in the microporous groups than in the macroporous group. There was no difference in bone formation between cylinders filled with DBBM or carbonate apatite. Conclusion: Microvasculature penetrates 50-μm diameter micropores and accelerates bone formation inside the cylinder, which was set on rat calvaria. The microporous titanium mesh can facilitate angiogenesis from both the dura mater and periosteal in vertical ridge augmentation. Our data showed superiority of microporous titanium vascular permeability and osteoconductivity, supporting bone growth.

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Section: Methodsmentioning

confidence: 99%

Section: A Circular Groove Was Made With a Trephine Bur And Five Small Holes Were Drilled With A Round Bur To Allow For Marrow Penetratiomentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Comparison of Macro-and Micro-porosity of a Titanium Mesh for Guided Bone Regeneration: AnIn VivoExperimental Study

Senoo

Hasuike

Yamamoto

et al. 2021

In Vivo

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Bone union‐promoting effect of romosozumab in a rat posterolateral lumbar fusion model

Kim

Inage

Mukaihata

et al. 2022

Journal Orthopaedic Research

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This study investigated the effect of romosozumab on bone union in a rat posterolateral lumbar fixation model. Posterolateral lumbar fixation was performed on 8‐week‐old male Sprague Dawley rats (n = 20). For bone grafting, autogenous bone (40 mg) was harvested from the spinous processes of the 10th thoracic vertebra until the 2nd lumbar vertebra and implanted between the intervertebral joints and transverse processes of the 4th and 5th lumbar vertebrae on both sides. Rats were matched by body weight and equally divided into two groups: R group (Evenity®, 25 mg/kg) and control (C) group (saline). Subcutaneous injections were administered twice a week until 8 weeks after surgery. Computed tomography was performed at surgery and week 8 after surgery. The area and percentage of bone trabeculae in the total area of bone fusion were calculated. Statistical analysis was performed using an unpaired t test (p < 0.05). We found that the R group rats had significantly higher mean bone union rate and volume than did the C group rats at all time courses starting week 4 after surgery. The R group had significantly higher increase rates than did the C group at weeks 4 and 6 after surgery. The percentage of bone trabeculae area in the R group was approximately 1.7 times larger than that in the C group. Thus, we demonstrated that romosozumab administration has stimulatory effects on bony outgrowth at bone graft sites. We attribute this to the modeling effect of romosozumab.

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Bone union‐promoting effect of romosozumab in an ovariectomized rat posterolateral lumbar fusion model

Okuyama,

Inage,

Kim

et al. 2024

Journal Orthopaedic Research

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Spinal fixation surgery has been increasingly performed in patients with osteoporosis. Romosozumab, a drug that was introduced in Japan recently, is known to possibly promote bone healing. However, few studies have reported the therapeutic effects of romosozumab in clinical practice in Japan. Therefore, here, we investigated the effects of romosozumab dosage on bone fusion promotion using an ovariectomized rat spinal fusion model. Eight‐week‐old female Sprague‐Dawley rats were matched by body weight and divided into three groups: 1.0 romosozumab (R) group (Evenity®, 25 mg/kg), 1/10R group (Evenity®, 2.5 mg/kg), and control (C) group (saline). Subcutaneous injections were administered twice a week for 8 weeks postoperatively. Computed tomography scans were performed every 2 weeks from the time of surgery till 8 weeks postoperatively. The mean fusion rates in terms of volume were significantly higher in the R groups [1/10R, 1.0R] than in the C group from 4 weeks postoperatively. The rate of increase was significantly higher in the 1.0R group from 4 weeks postoperatively and in the 1/10R group from 6 weeks postoperatively, than in the C group. The proportion of trabecular bone area was approximately 1.5 times higher in the R groups than in the C group. No significant differences were observed between the R groups. Our results suggest that romosozumab stimulates bone growth at the graft site, and similar effects were achieved at 1/10 of the standard dosage.

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Influences of mechanical barrier permeability on guided bone augmentation in the rat calvarium

Cited by 5 publications

References 16 publications

Comparison of Macro-and Micro-porosity of a Titanium Mesh for Guided Bone Regeneration: AnIn VivoExperimental Study

Comparison of Macro-and Micro-porosity of a Titanium Mesh for Guided Bone Regeneration: AnIn VivoExperimental Study

Bone union‐promoting effect of romosozumab in a rat posterolateral lumbar fusion model

Bone union‐promoting effect of romosozumab in an ovariectomized rat posterolateral lumbar fusion model

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