Influences of fermentation parameters on lovastatin production by Monascus purpureus using Saccharina japonica as solid fermented substrate

Suraiya, Sharmin; Kim, Jang-Ho; Tak, Jin Yeong; Siddique, Mahbubul Pratik; Young, Cho Ja; Kim, Joong Kyun; Kong, In‐Soo

doi:10.1016/j.lwt.2018.02.013

Cited by 43 publications

(22 citation statements)

References 32 publications

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“…With the increased attention paying to functional foods, some methods are developed to improve the production of MK in RMR, which could be used to further enhance MK content in foods. These methods include the optimization of culture medium (Panda, Javed, & Ali, 2010; Subhagar, Aravindan, & Viruthagiri, 2010; Suraiya et al, 2018) and the establishment of fermentation strategies in solid‐state fermentation (Gum, Nguyen, Lee, Han, & Cho, 2017; Lin, Wang, Li, Wu, & Chen, 2017; Tsukahara, Shinzato, Tamaki, Namihira, & Matsui, 2009). All these researches enhancing MK production in RMR were focusing on genes related to MK synthesis.…”

Section: Introductionmentioning

confidence: 99%

Effects of Chinese medicines on monacolin K production and related genes transcription of Monascus ruber in red mold rice fermentation

Lin

Ai‐lati

Liu

et al. 2020

Food Science & Nutrition

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Monacolin K (MK) is a secondary metabolite synthesized by polyketide synthases ofMonascus spp. In this study, the combined supplementation of three medicines, including Citri Reticulatae Pericarpium (CRP), Fructus crataegi (FC), and Radix Angelicae Dahuricae (RAD), were mixed with nonglutinous rice and were optimized by response surface methodology to enhance the production of MK in fermented red mold rice (RMR). Under the optimum condition, MK production achieved 3.60 mg/g, which was 41.18% higher than RMR without medicines. The improved MK production was mainly caused by the up-regulated transcription level of mokA, mokB, mokF, mokH, mokI, and mplaeA. Meanwhile, the inhibitory effect of Poria cocos (PC) on MK production (only 0.436 mg/g) was caused by significantly down-regulated transcription of six tested genes. Therefore, this study is beneficial for better understanding of the possible mechanism of enhanced MK production by optimization of fermentation conditions. K E Y W O R D SChinese medicine, monacolin K, Monascus ruber, red mold rice, response surface methodology | 2135 PENG Et al.

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Section: Introductionmentioning

confidence: 99%

Effects of Chinese medicines on monacolin K production and related genes transcription of Monascus ruber in red mold rice fermentation

Lin

Ai‐lati

Liu

et al. 2020

Food Science & Nutrition

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“…Moreover, the screening of Monascus strains with high-MK production has also been carried out (Suh et al, 2007;Wang et al, 2011). Novel substrates have been utilized for FRMR production and enriching its product types, such as Dioscorea, Finger millet, and Saccharina japonica (Lee et al, 2007;Venkateswaran and Vijayalakshmi, 2010;Suraiya et al, 2018). All these strategies head for high-quality FRMR.…”

Section: Introductionmentioning

confidence: 99%

Perspectives on Functional Red Mold Rice: Functional Ingredients, Production, and Application

Feng

2020

Front. Microbiol.

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Monacolin K (MK) is a secondary metabolite of the Monascus species that can inhibit cholesterol synthesis. Functional red mold rice (FRMR) is the fermentation product of Monascus spp., which is rich in MK. FRMR is usually employed to regulate serum cholesterol, especially for hypercholesterolemic patients who refuse statins or face statin intolerance. The present perspective summarized the bioactive components of FRMR and their functions. Subsequently, efficient strategies for FRMR production, future challenges of FRMR application, and possible directions were proposed. This perspective helps to understand the present situation and developmental prospects of FRMR.

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“…Moreover, the relative significance of each parameter can be evaluated by this method (Zhou et al 2009). Suraiya et al (2018) applied RSM method to optimize fermentation parameters on lovastatin production by Monascus purpureus using Saccharina japonica as solid fermented substrate. The experiments in which lovastatin was produced under optimal conditions yielding 13.98 mg/g dry fermented substrate corresponding to that of predicted combination of process parameters yielding 13.40 mg/g dry fermented substrate.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

Suwannarat¹,

Iewkittayakorn²,

Sukpondma³

et al. 2019

JSM

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The effect of lovastatin on cholesterol synthesis is well known. Although lovastatin has been produced from several Aspergillus species and cultivation systems, the yield of lovastatin from Aspergillus sclerotiorum PSU-RSPG178 in a static liquid system has not yet been determined. Therefore, the objective of this study was to optimize lovastatin production from this strain using a central composite design developed through response surface methodology (RSM). The inoculum was prepared from mycelium or spore cultured in static liquid and shaking systems with potato dextrose broth (PDB) as the medium. The best inoculum preparation and cultivation systems were selected to study the optimization of the four fermentation parameters of medium concentration (X-5X), temperature (25-37°C), culture medium to flask volume ratio (2.5-12.5) and time (7-35 days). The best inoculum preparation was the mycelium method and the best cultivation system was the static liquid system. In the optimization procedure, a yield of 1315.69 mg/L lovastatin was obtained at a PDB concentration of 5X, a temperature of 25°C, a medium to flask volume ratio of 1:2.5 and a time of 21 days. In a model employing the optimized parameters, temperature was found to have the most effect on lovastatin production, which will decrease if temperature increases above 25°C. Based on this study, A. sclerotiorum PSU-RSPG178 can be a useful strain for producing lovastatin and RSM as an optimization technique not only reduced the number of experiments but also resulted in an optimized lovastatin yield. ABSTRAK Kesan lovastatin pada sintesis kolesterol telah diketahui. Walaupun lovastatin dihasilkan daripada beberapa spesiesAspergillus dan sistem penanaman, hasil bagi lovastatin daripada Aspergillus sclerotiorum PSU-RSPG178 dalam sistem cecair statik masih belum ditentukan. Oleh itu, objektif kajian ini adalah untuk mengoptimumkan pengeluaran lovastatin daripada strain ini menggunakan reka bentuk komposit pusat yang dibangunkan melalui kaedah gerak balas permukaan (RSM). Inokulum disediakan daripada miselium atau spora dikultur dalam cecair statik dan sistem goncangan dengan kaldu kentang dektrosa (PDB) sebagai medium. Sistem penyediaan dan penanaman inokulum terbaik telah dipilih untuk kajian pengoptimuman empat parameter penapaian kepekatan sederhana (X-5X), suhu (25-37°C), kultur medium kepada nisbah isi padu kelalang (2.5-12.5) dan masa (7-35 hari). Persediaan inokulum terbaik adalah kaedah miselium dan sistem penanaman terbaik adalah sistem cecair statik. Dalam prosedur pengoptimuman, hasil lovastatin sebanyak 1315.69 mg/L telah diperoleh pada kepekatan PDB 5X, suhu 25°C, medium kepada nisbah isi padu kelalang 1:2.5 dan tempoh masa 21 hari. Model yang menggunakan parameter optimum, suhu didapati memberi kesan ketara pada pengeluaran lovastatin, yang akan berkurang jika suhu meningkat melebihi 25°C. Berdasarkan kajian ini, A. sclerotiorum PSU-RSPG178 boleh menjadi strain yang berguna untuk menghasilkan lovastatin dan RSM sebagai teknik pengoptimuman yang bukan s...

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Influences of fermentation parameters on lovastatin production by Monascus purpureus using Saccharina japonica as solid fermented substrate

Cited by 43 publications

References 32 publications

Effects of Chinese medicines on monacolin K production and related genes transcription of Monascus ruber in red mold rice fermentation

Effects of Chinese medicines on monacolin K production and related genes transcription of Monascus ruber in red mold rice fermentation

Perspectives on Functional Red Mold Rice: Functional Ingredients, Production, and Application

Optimization of the Production of Lovastatin from Aspergillus sclerotiorum PSU- RSPG178 under Static Liquid Culture using Response Surface Methodology

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