Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst development in vitro

Sirisathien, Saksiri; Hernández–Fonseca, Hugo; Brackett, Benjamin G.

doi:10.1016/s0378-4320(02)00272-5

Cited by 87 publications

(73 citation statements)

References 68 publications

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“…This is consistent with previous studies that showed a selective increase of cells in the ICM in bovine blastocysts (Sirisathien et al 2003) and embryos from other species treated with IGF1, including humans (Lighten et al 1998), pigs (Kim et al 2005) and mice (Smith et al 1993). The localised cell proliferation seems to be related to activation of the MAPK pathway in the ICM by IGF1 (Navarrete Santos et al 2008).…”

Section: Discussionsupporting

confidence: 93%

“…For instance, early embryos exposed to physiological concentrations of IGF1 (50-150 ng/ml) showed an increased number of cells in the resulting blastocysts in several mammalian species including mice (Lin et al 2003, Głabowski et al 2005, gerbil (Yoshida et al 2009), cattle (Byrne et al 2002, Makarevich & Markkula 2002, Sirisathien et al 2003, Jousan & Hansen 2007, buffaloes (Narula et al 1996), pigs (Kim et al 2005, rabbits (Herrler et al 1998) and humans (Lighten et al 1998). Physiological (100 ng/ ml) and supraphysiological (1000 ng/ml) concentrations of IGF1 increased the total cell number (TCN) in mice embryos in a dose-dependent manner (Głabowski et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Increased apoptosis in bovine blastocysts exposed to high levels of IGF1 is not associated with downregulation of the IGF1 receptor

Velazquez¹,

Hermann²,

Kues³

et al. 2011

Reproduction

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The hypothesis that high concentrations of IGF1 can impair embryo development was investigated in a bovine in vitro model to reflect conditions in polycystic ovary syndrome (PCOS) patients. Embryos were either cultured in the absence or presence of a physiological (100 ng/ml) or supraphysiological (1000 ng/ml) IGF1 concentration. Cell allocation, apoptosis, transcript and protein expression of selected genes involved in apoptosis, glucose metabolism and the IGF system were analysed. Supraphysiological IGF1 concentration did not improve blastocyst formation over controls, but induced higher levels of apoptosis, decreased TP53 protein expression in the trophectoderm and increased the number of cells in the inner cell mass (ICM). The increase in ICM cells corresponded with an increase in IGF1 receptor (IGF1R) protein in the ICM. A small, but significant, percentage of blastocysts displayed a hypertrophic ICM, not observed in controls and virtually absent in embryos treated with physiological concentrations of IGF1. Physiological IGF1 concentrations increased total IGF1R protein expression and upregulated IGFBP3 transcripts leading to an increase in blastocyst formation with no effects on cell number or apoptosis. In conclusion, the results support the hypothesis of detrimental effects of supraphysiological IGF1 concentrations on early pregnancy. However, our results do not support the premise that increased apoptosis associated with high levels of IGF1 is mediated via downregulation of the IGF1R as previously found in preimplantation mouse embryos. This in vitro system with the bovine preimplantation embryo reflects critical features of fertility in PCOS patients and could thus serve as a useful model for in-depth mechanistic studies.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Increased apoptosis in bovine blastocysts exposed to high levels of IGF1 is not associated with downregulation of the IGF1 receptor

Velazquez¹,

Hermann²,

Kues³

et al. 2011

Reproduction

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“…Although these studies showed that the addition of a high dose of EGF (100 ng/ml) into the culture medium had no remarkable effect on embryo development, we found that EGF at a concentration of 100 ng/ml or more induced a significant increase in the proportion of embryos at the blastocyst stage (21.3 and 29.7% for 100 and 200 ng/ml, respectively, vs. 11.9% for 0 ng/ ml on day 8) and that the numbers of cells in the ICM were also increased by supplementation with 1, 100 and 200 ng/ml (30.4, 32.1 and 32.5 for 1, 100 and 200 ng/ml, respectively, vs. 26.1 for 0 ng/ml). These results indicate that EGF is effective at influencing the rate of development to the blastocyst stage at concentrations of 100 ng/ml or more in our culture system.It is reported that IGF-I increases the numbers of cells in the ICM [18] and TE [28,42] and the total number of cells [19,29] in embryo culture. Pinto et al [43] suggested that IGF-I binds to receptors on the cell membrane to stimulate transportation of glucose transporter 8 and, as a result, inhibits apoptosis.…”

mentioning

confidence: 99%

“…In bovine embryos, a member of the EGF receptor subfamily, ErbB3, is expressed at the 2-cell embryo and blastocyst stages [25]. Insulin-like growth factor I (IGF-I) has also been reported to improve embryo development in humans [24], rabbits [26] and bovine species [18,19,21,27,28] and to suppress apoptosis in bovine embryos [19,28,29]. Expression of the IGF-I receptor has been identified at all stages of bovine embryo development from oocytes to blastocysts [25,30].…”

mentioning

confidence: 99%

Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and <i>In Vitro</i> Fertilization in Japanese Black Cows

Sakagami

Umeki

Nishino³

et al. 2012

J. Reprod. Dev.

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Abstract. The objective of this study was to examine whether high concentrations of epidermal growth factor (EGF) and/or insulin-like growth factor I (IGF-I) would have a beneficial effect on bovine embryo development in vitro and to obtain normal calves by using an ovum pick up method and embryo culture in a chemically defined medium. When compared with controls, EGF (100 or 200 ng/ml) or IGF-I (50 or 100 ng/ml) significantly increased the rate of embryos that developed into blastocysts during an 8-day culture after the in vitro fertilization of oocytes obtained from ovaries from a slaughterhouse. IGF-I induced a dose-dependent increase in cell number in both the inner cell mass and the trophectoderm, whereas EGF stimulated proliferation only in the inner cell mass. A combination of EGF (100 ng/ml) and IGF-I (50 ng/ml) produced an additive effect, and embryos developed into blastocysts at a comparatively high rate (27.9%) compared with controls (12.0%). A similar rate of development was achieved using a combination of EGF and IGF-I in the culture of embryos following ovum pick up by ultrasound-guided transvaginal follicular aspiration and in vitro fertilization, and 5 blastocysts that developed after the culture were transferred into uteri; two embryos implanted, and normal calves were born. These results suggest that the combined use of EGF and IGF-I makes bovine embryo culture in a chemically defined medium a practical and useful procedure for producing blastocysts, and its application to embryo culture following ovum pick up and in vitro fertilization could be useful for producing normal calves. Key words: Bovine embryo, Chemically defined medium, Epidermal growth factor (EGF), Insulin-like growth factor I (IGF-I), Ovum pick up (OPU) (J. Reprod. Dev. 58: [140][141][142][143][144][145][146] 2012) R ecently, a number of calves have been produced by the ovum pick up (OPU) method and in vitro culture (IVC) systems. Since the OPU method can be repeatedly applied to the same cow and can be used in pregnant [1] or problem cows in which embryos have not been obtained by uterine flushing after artificial insemination [2], this technology is effective for facilitating the improvement of domestic animals.An IVC system is necessary for the OPU method. Since successful pregnancy by transfer of embryos following the OPU method and in vitro fertilization has been confirmed [3], different protocols and medium conditions for oocyte maturation and embryo development have been tested [4][5][6][7]. However, there are some problems associated with the IVC system, such as a skewed sex ratio [8,9] or large offspring syndrome [10,11]. It has been suggested that factors in the culture medium such as serum used for embryo development may be involved in these problems. Fetal bovine serum (FBS) is widely employed as a supplement in the media used for culturing in vitro fertilized bovine embryos, but the subsequent rate of development of the fertilized oocytes to the blastocyst stage is known to vary among serum lots. There is thus a...

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“…According to several researchers (5,21,24), IGF-I and EGF had mitogenic effects and stimulated the growth and metabolism of embryos. These findings are consistent with our results.…”

Section: Discussionmentioning

confidence: 99%

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

Koçyiğit¹,

Çevik²,

Şen³

et al. 2015

Turk J Vet Anim Sci

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This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo culture. The quality of bovine embryos, based on the number of blastocyst cells and the ratio of ICM to total blastomeres, was affected by the sources of macromolecules and their combinations with growth factors. Growth factors supplemented to SOFaa media with BSA and PVA significantly increased the number of ICM cells and the ratio of ICM cells to total number of cells. In conclusion, replacing BSA with PVA depressed the blastocyst rate and cell numbers, and the number of blastomeres and ICM and TE cell numbers were affected by both the type of macromolecule and the growth factor supplements.

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Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst development in vitro

Cited by 87 publications

References 68 publications

Increased apoptosis in bovine blastocysts exposed to high levels of IGF1 is not associated with downregulation of the IGF1 receptor

Increased apoptosis in bovine blastocysts exposed to high levels of IGF1 is not associated with downregulation of the IGF1 receptor

Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and <i>In Vitro</i> Fertilization in Japanese Black Cows

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

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