Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and &lt;i&gt;In Vitro&lt;/i&gt; Fertilization in Japanese Black Cows

Sakagami, Nobutada; Umeki, Hidenobu; Nishino, Osamu; Uchiyama, Hiroko; Ichikawa, Kyoko; Takeshita, Kazunori; Kaneko, Etsushi; Akiyama, Kiyoshi; Kobayashi, S.; Tamada, Hiromichi

doi:10.1262/jrd.11-050m

Cited by 19 publications

(27 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with our results, Brice et al (1993) hypothesised that EGF receptor seemed to be more linked to the regulation of TE differentiation rather than cell proliferation in mouse preimplantation embryos. In agreement with our results but with larger groups of bovine embryos in culture (20 embryos/100 ml), a recent work found a significant effect of the combination IGF-I and EGF on embryo development in comparison with non-GF-supplemented (Sakagami et al, 2012). With regard to total cells per embryo, supplementation of IGF-I and EGF did not increase total cell number per blastocyst, only the ICM cell number (Sakagami et al, 2012).…”

Section: Discussionsupporting

confidence: 93%

“…In agreement with our results but with larger groups of bovine embryos in culture (20 embryos/100 ml), a recent work found a significant effect of the combination IGF-I and EGF on embryo development in comparison with non-GF-supplemented (Sakagami et al, 2012). With regard to total cells per embryo, supplementation of IGF-I and EGF did not increase total cell number per blastocyst, only the ICM cell number (Sakagami et al, 2012).On the other hand, the quality of embryos obtained at different days of culture was studied in the present work. We found that blastocyst at D7 had better quality, more cells per blastocyst and a lower apoptosis rate, than the rest of the blastocysts obtained later.…”

supporting

confidence: 93%

“…In particular, , in agreement with our results, did not find a significant effect of IGF-I with 10 ng/ml on embryo development (blastocyst from oocytes) when bovine embryos were cultured in larger groups (20 embryos/50 ml) in comparison with non-IGF-supplemented group. Recently, Sakagami et al (2012) found similar results to . Despite this, even using a high concentration, Block et al (2008) did not detect any significant effect of the supplementation of 100 ng/ml IGF-I on the blastocyst rate (25 embryos/50 ml) in comparison with the non-supplemented group.…”

supporting

confidence: 72%

See 2 more Smart Citations

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

View full text Add to dashboard Cite

When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

show abstract

Section: Discussionsupporting

confidence: 93%

supporting

confidence: 93%

supporting

confidence: 72%

See 1 more Smart Citation

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

View full text Add to dashboard Cite

show abstract

“…Actions of IGF1 on the preimplantation embryo indicate that both MAPK and PI3K/AKT pathways are activated (Figure 2). Culture with IGF1 increases the percent of embryos capable of developing to the blastocyst stage (28,30,(64)(65)(66)(67)(68)(69). This action of IGF1 probably involves increased proliferation because the cell number of day 6 morulae was increased by IGF1 (70).…”

Section: Insulin-like Growth Factormentioning

confidence: 99%

Maternal embryokines that regulate development of the bovine preimplantation embryo

Hansen¹,

Denicol²,

Dobbs³

2014

Turk J Vet Anim Sci

View full text Add to dashboard Cite

The zygote contains within its genome and epigenome the program to direct development through the embryonic, fetal, and postnatal periods. The execution of that program is dependent on the embryo's nongenetic inheritance from the oocyte and sperm (1,2) as well as on the environment in which development proceeds. Variation in the maternal environment can affect the ability of the preimplantation embryo to establish pregnancy. In cattle, the focus of this review, competence of the preimplantation embryo for growth and survival can be affected by maternal parity (3), lactational status (4), and circulating concentrations of progesterone (5). Moreover, patterns of gene expression in the endometrium are associated with survival of an embryo transferred to the uterus in the next ovulatory cycle (6,7).Development of the bovine embryo to the blastocyst in the absence of maternal signals (i.e. through in vitro production procedures) results in embryos with aberrant gene expression (8,9), lipid content (10,11), DNA methylation (12), and, as shown in the Table, reduced competence to establish pregnancy after transfer into recipients (13-17). Additionally, an increased proportion of embryos produced in vitro have developmental abnormalities that lead to increased neonatal death losses (18-20). Some of the problems with the in vitro-produced embryo could result from selection of incompetent oocytes or inadequate oocyte maturation. However, the importance of the maternal environment during development is indicated by observations that transfer of in vitro-produced embryos to the oviduct after fertilization limits some of the abnormalities associated with in vitro production (9,21).One function of the reproductive tract is to secrete bioactive molecules that regulate the embryo, oviduct, or endometrium. Genes for 115 ligands expressed in the endometrium had the corresponding receptor gene expressed by the embryo (22). Regulatory molecules produced by the oviduct and endometrium, which include hormones, growth factors, and cytokines, are referred to as embryokines when they function to regulate embryonic growth and development (23).It is likely that some of the variation in the ability of the reproductive tract to support embryonic development represents variation in secretion of embryokines. Indeed, several genes that were overexpressed in the endometrium of cows that subsequently became pregnant after embryo transfer as compared to cows that did not establish pregnancy are potential embryokines. These include NGF,

show abstract

“…These include insulin-like growth factor 1 (IGF1), epidermal growth factor (EGF), activin, granulocyte-macrophage colony-stimulating factor (GMCSF or CSF2), hyaluronan, fibroblast growth factor 2 (FGF2), and platelet-activating factor (PAF) (Gopichandran & Leese 2006, Fields et al 2011, Trigal et al 2011, Sakagami et al 2012). CSF2 has also been shown to act during gastrulation development (Loureiro et al 2009.…”

Section: Introductionmentioning

confidence: 99%

Hepatoma-derived growth factor: from the bovine uterus to the in vitro embryo culture

Gómez¹,

Correia-Álvarez²,

Caamaño³

et al. 2014

Reproduction

View full text Add to dashboard Cite

Early in cow embryo development, hepatoma-derived growth factor (HDGF) is detectable in uterine fluid. The origin of HDGF in maternal tissues is unknown, as is the effect of the induction on developing embryos. Herein, we analyze HDGF expression in day 8 endometrium exposed to embryos, as well as the effects of recombinant HDGF (rHDGF) on embryo growth. Exposure to embryos did not alter endometrial levels of HDGF mRNA or protein. HDGF protein localized to cell nuclei in the luminal epithelium and superficial glands and to the apical cytoplasm in deep glands. After uterine passage, levels of embryonic HDGF mRNA decreased and HDGF protein was detected only in the trophectoderm. In fetal fibroblast cultures, addition of rHDGF promoted cell proliferation. In experiments with group cultures of morulae in protein-free medium containing polyvinyl alcohol, adding rHDGF inhibited blastocyst development and did not affect cell counts when the morulae were early (day 5), whereas it enhanced blastocyst development and increased cell counts when the morulae were compact (day 6). In cultures of individual day 6 morulae, adding rHDGF promoted blastocyst development and increased cell counts. Our experiments with rHDGF indicate that the growth factor stimulates embryonic development and cell proliferation. HDGF is synthesized similarly by the endometrium and embryo, and it may exert embryotropic effects by autocrine and/or paracrine mechanisms.

show abstract

Normal Calves Produced After Transfer of Embryos Cultured in a Chemically Defined Medium Supplemented with Epidermal Growth Factor and Insulin-like Growth Factor I Following Ovum Pick Up and <i>In Vitro</i> Fertilization in Japanese Black Cows

Cited by 19 publications

References 51 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Maternal embryokines that regulate development of the bovine preimplantation embryo

Hepatoma-derived growth factor: from the bovine uterus to the in vitro embryo culture

Contact Info

Product

Resources

About