Influence of Watering Solution and Phenotype on the Growth of &amp;lt;i&amp;gt;in Vitro&amp;lt;/i&amp;gt; Propagated Pineapple (Smooth Cayenne Cultivar) Plantlets during Acclimatization

Agbidinoukoun, Arnaud; Ouikoun, Gaston C.; Mikpon, Toussaint; Kamade, Gatien T.; Badou, Bienvenu T.; Aïsso, Richmy C. B.; Houédjissin, Serge S.; Houngue, Jerome Anani; Dossoukpevi, R; Ahanhanzo, Corneille

doi:10.4236/as.2021.1211077

Cited by 2 publications

(3 citation statements)

References 18 publications

(11 reference statements)

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“…Previous studies by Hu et al [47], Souza et al [10] and Villalobos-Olivera et al [12] described in vitro pineapple plants regenerated from shoot tip cryopreservation, but the adaptation of in vitro to ex vitro acclimatization conditions was not addressed. The success of in vitro propagation systems depends on effective acclimatization and this step is also critical for the establishment of seedlings under field conditions [48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

et al. 2023

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Studies on the morpho-physiology of cryo-derived pineapple plants after acclimatization have been quite limited. Therefore, in the present study, the morpho-anatomical and physiological characteristics of cryo-derived Ananas comosus var. comosus ‘MD-2’ plants after acclimatization were investigated. Plants obtained from cryopreserved and non-cryopreserved shoot tips, as well as in vitro stock cultures (control), showed similar morphological development (viz. plant height, number of leaves, D leaf length, D leaf width, D leaf area, diameter of stem base, number of roots, plant fresh weight and plant dry weight) to conventionally micropropagated and non-cryopreserved plants. The pineapple plantlets developed efficient anatomical leaf structures that allowed them to adapt to the transition process from in vitro to ex vitro. In all groups of plants, the content of water and chlorophylls (a, a + b, a/b) decreased during the first 15 days of acclimatization and then remained constant until the end of the evaluation. The mesophilic succulence index increased to its maximum value after 15 days, then decreased and remained constant up to 45 days. Although physiological indicators fluctuated during the 45 days of acclimatization, no differences were observed in any of the indicators evaluated when plantlets obtained from cryopreserved shoot tips were compared with controls. The results of the plants from cryopreserved shoot tips show that they switched from C3 to Crassulacean acid metabolism, which denoted metabolic stability during acclimatization.

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Section: Discussionmentioning

confidence: 99%

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

et al. 2023

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“…The rooted plantlets were removed from tubes and successfully rinsed in clean water and planted in a horticultural socket containing a sterilized substrate of sawdust and compost as described by Agbinoukoun et al [27]. The plantlets were maintained under greenhouse respecting plant growing conditions (T = 29˚C; Humidity of 70.2%) for eight weeks.…”

Section: Acclimatization Des Vitroplantsmentioning

confidence: 99%

“…The plantlets were maintained under greenhouse respecting plant growing conditions (T = 29˚C; Humidity of 70.2%) for eight weeks. Plantlets were periodically watered with Shive solution as described by Agbinoukoun et al [27].…”

Section: Acclimatization Des Vitroplantsmentioning

confidence: 99%

Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (<i>Ananas comosus</i> L.)

Cacaï¹,

Ahokpossi²,

Houédjissin³

et al. 2023

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Unavailability of performant planting material of pineapple constitutes a major problem of its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the in vitro responses of two cultivars of pineapple during the explant's regeneration. Calli were induced from crown leaf and plantlets leaf of "Smooth Cayenne" and "Sugarloaf cultivars". Murashige and Skoog medium with vitamins B5 supplemented with different growth regulators combinations were used. BAP and/or 2,4-D have been added to base medium for calli cells' differentiation while BAP and GA3 have been added for plant elongation. The results indicated that explants from regenerated plantlets leaves cultivated on MS supplemented with copper (II) sulphate 5-hydrate concentrations incorporated had significant (p < 0.0001) influence on callus induction in pineapple cultivars. Likewise, MS medium with NAA (0.5 mg/l) + BAP (1 mg/l) had a highly significant influence with 8.8 differentiated Calli. Also, MS medium supplemented with BAP (3 mg/l) + GA3 (2 mg/l) for the "smooth Cayenne" had significantly influenced (p < 0.0001) Calli regeneration with a high rate of 55.25% plantlets. MS medium containing 0.5 mg/l of NAA + 0 mg/l IBA produced a high number of roots in Sugarloaf whereas the medium containing 1.5 mg/l NAA + 0.5 mg/l (IBA) produced high number of roots in smooth Cayenne. We have established an efficient and reproducible protocol for mass propagation and genetic transformation of pineapple though indirect organogenesis. This protocol may be used in genetics engineering studies for pineapple breeding.

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Influence of Watering Solution and Phenotype on the Growth of <i>in Vitro</i> Propagated Pineapple (Smooth Cayenne Cultivar) Plantlets during Acclimatization

Cited by 2 publications

References 18 publications

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (<i>Ananas comosus</i> L.)

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Influence of Watering Solution and Phenotype on the Growth of &lt;i&gt;in Vitro&lt;/i&gt; Propagated Pineapple (Smooth Cayenne Cultivar) Plantlets during Acclimatization

Cited by 2 publications

References 18 publications

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

Morpho-Anatomical and Physiological Assessments of Cryo-Derived Pineapple Plants (Ananas comosus var. comosus) after Acclimatization

Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (&lt;i&gt;Ananas comosus&lt;/i&gt; L.)

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Influence of Watering Solution and Phenotype on the Growth of <i>in Vitro</i> Propagated Pineapple (Smooth Cayenne Cultivar) Plantlets during Acclimatization

Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (<i>Ananas comosus</i> L.)