Influence of water chemistry and migratory distance on ability to distinguish progeny of sympatric resident and anadromous rainbow trout (<i>Oncorhynchus mykiss</i>)

Donohoe, Christopher J.; Adams, P.; Royer, Chantell F.

doi:10.1139/f08-029

Cited by 30 publications

(43 citation statements)

References 34 publications

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“…Otoliths were prepared following methods in Zimmerman and Reeves (2000) and Donohoe et al (2008). We cleaned and mounted at least one otolith from each fish, with the sulcus side facing up.…”

Section: Data Collectionmentioning

confidence: 99%

“…Probed points with total weight percent below 90% or above 110% were discarded. Some freshwater areas have high Sr:Ca ratios that may obscure maternal origin (Rieman et al 1994;Donohoe et al 2008). To avoid misidentification of maternal origin resulting from unknown freshwater microchemistry, we tested Sr:Ca ratios in water samples from juvenile sampling sites.…”

Section: Data Collectionmentioning

confidence: 99%

“…After transporting samples from the field, we stored samples in a 40°cold room until analysis with a Varian AA240FS flame atomic absorption spectroscope (Varian, Inc, Palo Alto, California). We calculated mean ionic concentration and molar ratios of Sr:Ca for each site, and compared the results for each site to an anadromy detection threshold of 4.75 mmol/mol: roughly half the Sr: Ca ratio in the Pacific Ocean (de Villiers 1999;Zimmerman 2005;Donohoe et al 2008). We did not use fish samples from sites with water Sr:Ca ratios over this threshold.…”

Section: Data Collectionmentioning

confidence: 99%

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Variability in expression of anadromy by female Oncorhynchus mykiss within a river network

et al. 2011

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We described and predicted spatial variation in marine migration (anadromy) of female Oncorhynchus mykiss in the John Day River watershed, Oregon. We collected 149 juvenile O. mykiss across 72 sites and identified locations used by anadromous females by assigning maternal origin (anadromous versus non-anadromous) to each juvenile. These assignments used comparisons of strontium to calcium ratios in otolith primordia and freshwater growth regions to indicate maternal origin.We used logistic regression to predict probability of anadromy in relation to mean annual stream runoff using data from a subset of individuals. This model correctly predicted anadromy in a second sample of individuals with a moderate level of accuracy (e.g., 68% correctly predicted with a 0.5 classification threshold). Residuals from the models were not spatially autocorrelated, suggesting that remaining variability in the expression of anadromy was due to localized influences, as opposed to broad-scale gradients unrelated to mean annual stream runoff. These results are important for the management of O. mykiss because anadromous individuals (steelhead) within the John Day River watershed are listed as a threatened species, and it is difficult to discern juvenile steelhead from non-anadromous individuals (rainbow trout) in the field. Our results provide a broadscale description and prediction of locations supporting anadromy, and new insight for habitat restoration, monitoring, and research to better manage and understand the expression of anadromy in O. mykiss.

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Section: Data Collectionmentioning

confidence: 99%

Section: Data Collectionmentioning

confidence: 99%

Section: Data Collectionmentioning

confidence: 99%

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Variability in expression of anadromy by female Oncorhynchus mykiss within a river network

et al. 2011

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“…On the other hand, Reeves (2000, 2002) were successful at discriminating anadromous and non-anadromous Oncorhynchus mykiss. The limitation is due to the fact that Sr:Ca in the core of an otolith does not depend solely on the maternal origin but also on background concentrations in freshwater, migratory distances, and the length of the time in freshwater prior to spawning (Donohoe et al 2008). δ 34 S offers a promising alternative to elemental signatures since organic sulfur in animal tissue is derived from organic sulfur in their diet and δ 34 S differs between marine and freshwater environments with little to no trophic fractionation and temperature effect (Peterson and Fry 1987;Hesslein et al 1991;Barnes and Jennings 2007).…”

Section: Introductionmentioning

confidence: 99%

Sulfur isotopes in otoliths allow discrimination of anadromous and non-anadromous ecotypes of sockeye salmon (Oncorhynchus nerka)

et al. 2010

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Oncorhynchus nerka occur both as anadromous sockeye salmon that spend most of their life in the ocean, and as non-anadromous kokanee salmon that remain in fresh water their entire lives. We assessed whether stable isotopes of sulfur (δ 34 S) in otoliths could be used to distinguish sockeye salmon and kokanee ecotypes that are otherwise difficult to identify when they share a common freshwater rearing environment. We also investigated the chemical link between salmon and their diet by measuring δ 34 S in various fish tissues (eggs, muscle, scales) and zooplankton. δ 34 S (mean±SE) in sockeye salmon eggs (18.7±0.4‰) and marine zooplankton (20.5±0.1‰) were enriched by 10-14‰ compared with kokanee eggs and freshwater zooplankton. δ 34 S in the otolith cores of sockeye salmon (19.2±0.7‰) and kokanee salmon (5.3±1.1‰) were similar to δ 34 S in marine and freshwater zooplankton, respectively, indicating that the core is derived from maternal yolk tissue and reflects the maternal diet. δ 34 S in the freshwater growth zone of otoliths did not differ significantly between sockeye (5.9±1.1‰) and kokanee salmon (4.4± 1.2‰), and was similar to freshwater zooplankton. The mean difference between δ 34 S in the otolith core and first year of growth was 13.3±1.4‰ for sockeye and 0.65±1.3‰ for kokanee salmon. A quadratic discriminant function developed from measurements of δ 34 S in otoliths of known maternal origin provided perfect classification rates in cross-validation tests. Thus, sulfur isotope ratios in otoliths are effective in discriminating between anadromous and nonanadromous ecotypes of O. nerka.

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“…Commonly used methods include wavelength dispersive electron microprobe analysis (WD-EMPA), ion microprobe analyses such as proton-induced X-ray emission (PIXE) and secondary ion mass spectrometry (SIMS), and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) (Campana et al 1997;Weber et al 2002;Morris et al 2005;Donohoe et al 2008). Beam-based methods are often favored because small beam diameters allow for high spatial resolution and can produce detailed chronologies of elemental and isotopic variation within specific otolith regions or over the life of a fish (Zimmerman and Reeves 2002;Elsdon et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A method of mounting multiple otoliths for beam-based microchemical analyses

Donohoe

Zimmerman

2010

Environ Biol Fish

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Beam-based analytical methods are widely used to measure the concentrations of elements and isotopes in otoliths. These methods usually require that otoliths be individually mounted and prepared to properly expose the desired growth region to the analytical beam. Most analytical instruments, such as LA-ICPMS and ion and electron microprobes, have sample holders that will accept only one to six slides or mounts at a time. We describe a method of mounting otoliths that allows for easy transfer of many otoliths to a single mount after they have been prepared. Such an approach increases the number of otoliths that can be analyzed in a single session by reducing the need open the sample chamber to exchange slides-a particularly time consuming step on instruments that operate under vacuum. For ion and electron microprobes, the method also greatly reduces the number of slides that must be coated with an electrical conductor prior to analysis. In this method, a narrow strip of cover glass is first glued at one end to a standard microscope slide. The otolith is then mounted in thermoplastic resin on the opposite, free end of the strip. The otolith can then be ground and flipped, if needed, by reheating the mounting medium. After otolith preparation is complete, the cover glass is cut with a scribe to free the otolith and up to 20 small otoliths can be arranged on a single petrographic slide.

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Influence of water chemistry and migratory distance on ability to distinguish progeny of sympatric resident and anadromous rainbow trout (Oncorhynchus mykiss)

Cited by 30 publications

References 34 publications

Variability in expression of anadromy by female Oncorhynchus mykiss within a river network

Variability in expression of anadromy by female Oncorhynchus mykiss within a river network

Sulfur isotopes in otoliths allow discrimination of anadromous and non-anadromous ecotypes of sockeye salmon (Oncorhynchus nerka)

A method of mounting multiple otoliths for beam-based microchemical analyses

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