Influence of volume medium on growth and ginsenoside level in adventitious root culture of Panax ginseng CA Meyer

Abstract

Ginseng (Panax ginseng, Family Araliaceae) is a traditional herbal plant that is pretty well known and has been widely used in various countries, such as Korea, China, and Japan. Ginseng contains ginsenoside secondary metabolites that have been shown to have therapeutic effects, such as antioxidant, anti-inflammatory, anti-allergic, anti-diabetic, and anti-cancer. Ginseng production by traditional cultivation methods is long and produces inconsistent results. Therefore, in vitro culture is an alternative method to produce ginseng and ginsenoside consistently. In 2018, PT Bintang Todjoe collaborated with the University of Surabaya (UBAYA) and the Hanbang-Bio Inc. (holding company of Kyung Hee University) to establish the Kalbe Ubaya Hanbang-Bio Laboratory (KUH Lab). From previous studies, the dry weight achieved was only 109.758 g, which did not reach the target (120 grams). Therefore, the media was modified by adding media volume from 13L to 15L. The increase in media volume increased fresh weight to 2728.7 g, dry weight to 137.6 g, and yield up to 5%. However, this increase in media volume has not increased ginsenoside levels.

In Vitro Cultivation and Ginsenosides Accumulation in Panax ginseng: A Review

“…In addition, under controlled culture conditions, numerous factors influence the quantity and quality of ginsenosides, such as medium constituents, pH, light conditions, culture temperature, explants, and abiotic factors. [32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36]. [32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36].…”

“…[32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36]. [32], (b) somatic embryos (bar 1 mm) [33], (c) shoots (bar 1 cm) [32], (d) hairy roots (bar 820 µm) [34], (e) cell suspension [35], and (f) adventitious roots [36].…”

“…Types of P. ginseng tissue culture. Note: (a) Callus (bar 1 cm)[32], (b) somatic embryos (bar 1 mm)[33], (c) shoots (bar 1 cm)[32], (d) hairy roots (bar 820 µm)[34], (e) cell suspension[35], and (f) adventitious roots[36].…”