Influence of Various Factors on Polysaccharide Synthesis in S. Mitis

Weiss, Sidney; King, William J.; Kestenbaum, R.C.; Donohue, John J.

doi:10.1111/j.1749-6632.1965.tb34849.x

Cited by 38 publications

(20 citation statements)

References 9 publications

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“…The resting cells in our experiments contained little or no polysaccharide initially and are assumed to have a low ATP content. The polysaccharide content of cells of streptococci, allowed to accumulate polysaccharide, may constitute over 50 per cent of the total dry weight of the cells (GIBBONS, 1964a;VAN HOUTE, 1967), while 35 per cent of the total glucose utilized is converted into polysaccharide (WEISS et aL, 1965). Therefore, polysaccharide synthesis by the cell places a great demand on the available ATP which may result in an increased rate of glycolysis.…”

Section: Polysaccharide Synthesis and Acid Productionmentioning

confidence: 99%

Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci

Houte

Winkler

Jansen

1969

Archives of Oral Biology

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Sammary--The relation between iodophilic polysaccharide formation, acid production and growth in a-haemolytic streptococci, isolated from human dental plaque, was studied. In experiments with resting cell suspensions, or with cells growing at a low rate, all strains synthesizing iodophilic polysaccharide were found to be more acidogenic than strains not producing iodophilic polysaccharide. The higher acid production rate of the iodophilic polysaccharide-producing strains seems to be the result of their increased demand for energy required for polysaccharide synthesis. In cultures of iodophilic polysaccharide-storing strains, with a high initial cell concentration (4-10 l° cells]ml), cell multiplication was delayed and did not occur before the polysaccharide content of the cells was at or near its maximal level. No such delay was observed in cultures of strains not producing iodophilic polysaccharide. With regard to the importance of iodophilic polysaccharide synthesis for the carious process, the acid production from polysaccharide degradation has, up till now, been stressed. The present results indicate that in addition to this process, also the high acid production rate of iodophilic polysaccharide-storing streptococci during polysaccharide synthesis, may increase the acidogenic potential of dental plaques containing such organisms.

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Section: Polysaccharide Synthesis and Acid Productionmentioning

confidence: 99%

Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci

Houte

Winkler

Jansen

1969

Archives of Oral Biology

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“…Caries inhibition by fluoride may also result from antimicrobial ef fects which can influence the metabolism of plaque microorganisms [Fitzgerald and Jordan, 1953;Weiss et al, 1965;Jenkins and Edgar, 1969;Kanapka and Hamilton, 1969;Sandham and Kleinberger, 1973;Bowen and Hewitt, 1974] or reduce the number of cariogenic microor ganisms on the tooth surface [Birkeland, 1972]. None of these proposed mechanisms of action is incompatible with the others, and all may actual ly be operative at the same time.…”

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Caries Inhibition in the Rat by Water-Borne and Enamel-Bound Fluoride

Larson¹,

Mellberg²,

Englander³

et al. 1976

Caries Res

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Osborne-Mendel rats 22–25 days of age were subjected to a 7-day precaries test period during which they were fed a high starch diet and drank distilled water containing no added fluoride or with 10, 50, 100 or 150 ppm fluoride as sodium fluoride. After this period, 36 animals from each group were provided with distilled water during a 56-day caries test period (fed diet 2000 and infected with Streptococcus mutans 6715). Fluoride content in the enamel was shown to be directly related to the level of fluoride in the drinking water during the initial 7-day exposure period, both at the end of this 7-day period of fluoride treatment and at the end of the caries test period. Caries development was inversely related to fluoride uptake in the enamel; animals which received 50 ppm or more fluoride developed significantly fewer carious areas than the controls. 36 additional animals from each pretest group were subjected to the same caries test challenge as above but in addition were provided with 10 ppm fluoride in the drinking water. Caries development in these groups was extremely low even though there was no increase in the fluoride content of enamel in animals drinking water containing 10 ppm fluoride during the caries test period. Fluoride provided prior to the caries test regimen conferred the most protection on the sulci, in contrast, fluoride provided during the caries test regimen had a more pronounced effect on the smooth surfaces and in addition had a greater overall effect.

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“…The fluoride content in plaque is significantly higher in fluoridated localities than in control areas [Dawes et al, 1965], A larger amount of fluoride ions is supposed to be bound to or within the microbial cells [Jenkins et al, 1969]. These con centrations of F~ ions were found to influence both the microbial com position of plaque [de Stoppelaar et al, 1969] and the metabolic activ ity of plaque streptococci and other oral microbiota [Weiss et al, 1965;Sims, 1966; E dgar et al, 1970], The importance of microbial polysac charides for the formation of dental plaque and for the mechanism of …”

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Amount and Distribution of Extracellular Polysaccharides in Dental Microbial Plaque

Broukal¹,

Zajicek²

1974

Caries Res

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Extracellular polysaccharides (EPS) in human dental plaque were investigated in relation to fluoride concentration in drinking water under both natural and experimental conditions. In a low-fluoride area (0.2 ppm F^––) the level of EPS amounted to 15.2% and in a fluoridated area (1.0 ppm F^––) to 10.3% of the dry weight of samples (a significant difference). Under experimental conditions (after mouth-rinsing with fluoride-sucrose solutions), a significant influence of F^–– on the level of EPS could only be observed at a concentration of 10.0 ppm. The ratio of water-soluble and alkali-soluble fractions was approximately 60:40 in both natural and experimental plaques.

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Influence of Various Factors on Polysaccharide Synthesis in S. Mitis

Cited by 38 publications

References 9 publications

Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci

Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci

Caries Inhibition in the Rat by Water-Borne and Enamel-Bound Fluoride

Amount and Distribution of Extracellular Polysaccharides in Dental Microbial Plaque

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