Influence of Various Adjuvants on the Synthesis of Specific Antibodies of Chicken, Sheep and Rabbit Following Immunization with an Hapten*

Erhard, Michael; Kellner, Julia; Kühlmann, R.; Lösch, U.

doi:10.1111/j.1439-0442.1991.tb00979.x

Cited by 7 publications

(3 citation statements)

References 15 publications

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“…To ex- Our data suggest that the source of the carrier protein primarily affects the antibody amount generated. Investigations similar to those described here using a non-rabbit protein as carrier led to considerably higher titers (12) but not to significant changes in antibody affinity. Consistent with a previous study (21), we found that a carrier protein derived from the same species as the immune host is immunologically not inert.…”

Section: Measurement Of Relative Antibody Affinity Distributionsupporting

confidence: 51%

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The Specificity and Affinity of Polyclonal Antibodies Raised in Rabbits Against a Hapten Conjugated to Rabbit Serum Albumin

Schöneberg

Erhard²,

Kellner³

et al. 1995

Journal of Immunoassay

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In this study, rabbit antisera to hapten-rabbit serum albumin conjugates were investigated regarding antibody titer, affinity, specificity, and affinity distribution. Methyl phosphonic acid p-amino-phenyl 1,2,2-trimethylpropyldiester (MATP) served as model hapten. Four MATP-rabbit serum albumin conjugates with various hapten densities (with and without spacer) were synthesized and used for immunization of rabbits. Antisera were collected over a 130 day-period and characterized with different ELISA methods. We found that immunogens with rabbit serum albumin gave antisera with lower titers, but similar affinity as compared to polyclonal or monoclonal antibodies obtained with non-rabbit protein as carrier protein. Immunogens with a low hapten density led to higher final titers without affecting antibody affinity or specificity. Immunogens containing a bridging group resulted in higher antibody affinity with a changed specificity. The pattern of antibody affinity distribution differed considerably among individual rabbits and showed a non-Gaussian subpopulation distribution.

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Section: Measurement Of Relative Antibody Affinity Distributionsupporting

confidence: 51%

“…(15). The production of anti-MATP polyclonal or monoclonal antibodies (mAbs) obtained by using non-rabbit protein as carrier protein has been described recently (12,18,19). These rabbit antisera and four …”

Section: Syntheses Of Hapten-carrier Conjugatesmentioning

confidence: 99%

The Specificity and Affinity of Polyclonal Antibodies Raised in Rabbits Against a Hapten Conjugated to Rabbit Serum Albumin

Schöneberg

Erhard²,

Kellner³

et al. 1995

Journal of Immunoassay

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“…TiterMax™, Ribi™, and Gerbu™ adjuvants) have been invented to generate a potent immune response with significantly reduced toxicity (Stils, 2005;Algate et al, 2013), but most of the evaluation studies were focused on developing antibodies to macromolecules. Only a few investigations have been carried out to find replacements for Freund's adjuvants in the production of hapten-specific antibodies (Erhard et al, 1991;Fodey et al, 2008;George et al, 2012;Maquieira et al, 2012;Parra et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Liu

Lan

et al. 2016

J. Zhejiang Univ. Sci. B

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A water-soluble adjuvant named QuickAntibody (QA) was introduced into the procedure of mouse immunization for the development of hapten-specific monoclonal antibodies (mAbs), using four kinds of pesticides as model compounds. Compared with conventional Freund's adjuvants, QA treatments offered relatively low but acceptable antiserum titers after three inoculations, gave little adverse effects to the experimental animals, and were preferable in harvesting splenocytes during the steps of cell fusion. Afterwards, hybridomas from the QA group were prepared and screened by both non-competitive and competitive indirect enzyme-linked immunosorbent assays (ELISAs). The efficiency of gaining immune-positive hybridomas was satisfactory, and the resultant mAbs showed sensitivities (half maximal inhibitory concentration (IC 50)) of 0.91, 2.46, 3.72, and 6.22 ng/ml to triazophos, parathion, chlorpyrifos, and fenpropathrin, respectively. Additionally, the performance of QA adjuvant was further confirmed by acquiring a high-affinity mAb against okadaic acid (IC 50 of 0.36 ng/ml) after three immunizations. These newly developed mAbs showed similar or even better sensitivities compared with previously reported mAbs specific to the corresponding analytes. This study suggested that the easy-to-use adjuvant could be applicable to the efficient generation of highly sensitive mAbs against small compounds.

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Immunisation

Schwarzkopf

Staak

Behn

et al. 2001

Chicken Egg Yolk Antibodies, Production and Application

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Influence of Various Adjuvants on the Synthesis of Specific Antibodies of Chicken, Sheep and Rabbit Following Immunization with an Hapten*

Cited by 7 publications

References 15 publications

The Specificity and Affinity of Polyclonal Antibodies Raised in Rabbits Against a Hapten Conjugated to Rabbit Serum Albumin

The Specificity and Affinity of Polyclonal Antibodies Raised in Rabbits Against a Hapten Conjugated to Rabbit Serum Albumin

Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds

Immunisation

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