Influence of transgenic Bt176 and non-transgenic corn silage on the structure of rumen bacterial communities

Brusetti, Lorenzo; Crotti, Elena; Tamburini, A.; Cittaro, Davide; Garavaglia, Virgilio; Rolli, Eleonora; Sorlini, C.; Daffonchio, Daniele; Borin, Sara

doi:10.1007/s13213-011-0215-8

Cited by 14 publications

(15 citation statements)

References 34 publications

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“…Similar results have been obtained in cows, where shortterm consumption of GM maize did not affect rumen microbiota as assessed by 16S rRNA gene sequencing (11), quantitative PCR (12), or ribosomal intergenic spacer analysis (30). Culturable amylolytic and cellulolytic bacteria were also unaffected by 3 years of feeding GM maize to sheep (6).…”

Section: Discussionsupporting

confidence: 75%

“…As the maternal microbiota provides the inoculum for colonization of the offspring digestive tract (19), any effect that GM maize consumption may have on the intestinal microbiota of pregnant sows could affect the health and lifelong performance of their offspring. A number of studies have investigated the impact of Bt maize consumption on the intestinal microbiota of ruminants (11,12,30), and our group has evaluated its influence on the porcine microbiota (13,14). However, results from transgenerational studies are absent from the literature, and, to our knowledge, this is the first study to investigate the effects of GM maize on the intestinal microbiota of pregnant sows and their offspring.…”

Section: Discussionmentioning

confidence: 99%

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Sequence-Based Analysis of the Intestinal Microbiota of Sows and Their Offspring Fed Genetically Modified Maize Expressing a Truncated Form of Bacillus thuringiensis Cry1Ab Protein (Bt Maize)

Buzoianu

Walsh

Rea

et al. 2013

Appl Environ Microbiol

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eThe aim was to investigate transgenerational effects of feeding genetically modified (GM) maize expressing a truncated form of Bacillus thuringiensis Cry1Ab protein (Bt maize) to sows and their offspring on maternal and offspring intestinal microbiota. Sows were assigned to either non-GM or GM maize dietary treatments during gestation and lactation. At weaning, offspring were assigned within sow treatment to non-GM or GM maize diets for 115 days, as follows: (i) non-GM maize-fed sow/non-GM maize-fed offspring (non-GM/non-GM), (ii) non-GM maize-fed sow/GM maize-fed offspring (non-GM/GM), (iii) GM maize-fed sow/non-GM maize-fed offspring (GM/non-GM), and (iv) GM maize-fed sow/GM maize-fed offspring (GM/GM). Offspring of GM maize-fed sows had higher counts of fecal total anaerobes and Enterobacteriaceae at days 70 and 100 postweaning, respectively. At day 115 postweaning, GM/non-GM offspring had lower ileal Enterobacteriaceae counts than non-GM/non-GM or GM/GM offspring and lower ileal total anaerobes than pigs on the other treatments. GM maize-fed offspring also had higher ileal total anaerobe counts than non-GM maize-fed offspring, and cecal total anaerobes were lower in non-GM/GM and GM/ non-GM offspring than in those from the non-GM/non-GM treatment. The only differences observed for major bacterial phyla using 16S rRNA gene sequencing were that fecal Proteobacteria were less abundant in GM maize-fed sows prior to farrowing and in offspring at weaning, with fecal Firmicutes more abundant in offspring. While other differences occurred, they were not observed consistently in offspring, were mostly encountered for low-abundance, low-frequency bacterial taxa, and were not associated with pathology. Therefore, their biological relevance is questionable. This confirms the lack of adverse effects of GM maize on the intestinal microbiota of pigs, even following transgenerational consumption.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Sequence-Based Analysis of the Intestinal Microbiota of Sows and Their Offspring Fed Genetically Modified Maize Expressing a Truncated Form of Bacillus thuringiensis Cry1Ab Protein (Bt Maize)

Buzoianu

Walsh

Rea

et al. 2013

Appl Environ Microbiol

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“…Einspanier et al (11) found no effect of 28 days of feeding Bt maize to cows on ruminal bacterial communities using 16S rRNA gene sequencing. Similarly, ribosomal intergenic spacer analysis revealed no effects of feeding Bt 176 maize silage for 35 days on ruminal bacterial population structure in cows (6). Likewise, Trabalza-Marinucci et al (51) reported no effect of feeding Bt maize for 36 months on the ruminal microbiota of sheep, as revealed by culturing.…”

mentioning

confidence: 98%

High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days

Buzoianu

Walsh

Rea

et al. 2012

Appl Environ Microbiol

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The objective of this study was to investigate if feeding genetically modified (GM) MON810 maize expressing the Bacillus thuringiensis insecticidal protein (Bt maize) had any effects on the porcine intestinal microbiota. Eighteen pigs were weaned at ϳ28 days and, following a 6-day acclimatization period, were assigned to diets containing either GM (Bt MON810) maize or non-GM isogenic parent line maize for 31 days (n ‫؍‬ 9/treatment). Effects on the porcine intestinal microbiota were assessed through culture-dependent and -independent approaches. Fecal, cecal, and ileal counts of total anaerobes, Enterobacteriaceae, and Lactobacillus were not significantly different between pigs fed the isogenic or Bt maize-based diets. Furthermore, high-throughput 16S rRNA gene sequencing revealed few differences in the compositions of the cecal microbiotas. The only differences were that pigs fed the Bt maize diet had higher cecal abundance of Enterococcaceae (0.06 versus 0%; P < 0.05), Erysipelotrichaceae (1.28 versus 1.17%; P < 0.05), and Bifidobacterium (0.04 versus 0%; P < 0.05) and lower abundance of Blautia (0.23 versus 0.40%; P < 0.05) than pigs fed the isogenic maize diet. A lower enzyme-resistant starch content in the Bt maize, which is most likely a result of normal variation and not due to the genetic modification, may account for some of the differences observed within the cecal microbiotas. These results indicate that Bt maize is well tolerated by the porcine intestinal microbiota and provide additional data for safety assessment of Bt maize. Furthermore, these data can potentially be extrapolated to humans, considering the suitability of pigs as a human model.

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“…11,12 Transgenic insect-resistant corn and transgenic herbicide-resistant corn are the most extensively cultivated GM corn varieties and have great potential for popularization in China. 1 There have been lots of feeding trials focusing on a safety evaluation of transgenic insect-resistant corn with respect to gut bacteria [13][14][15][16] and a few studoes reported no completely consistent effects on the intestinal bacteria of animals. However, most of the differences were regarded to have no negative effects on animal health.…”

Section: Introductionmentioning

confidence: 99%

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

et al. 2020

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BACKGROUND: The present study investigated the chronic effect on the composition and proportions of the cecal microbiota of laying hens for 12 weeks after consuming two genetically modified (GM) corns containing the maroACC gene from the Agrobacterium tumefaciens strain (CC) and the mCry1Ac gene from the Bacillus thuringiensis strain (BT) in comparison with the isogenic corn (CT). RESULTS: In total, 72 hens were randomly assigned to the CT corn-based diet, CC corn-based diet and BT corn-based diet. The absolute weights of abdominal fat, breast, thigh meat and organ weight were not affected by the dietary treatment. Highthroughput 16S rRNA gene sequencing revealed a few differences in the composition of cecal microbiota among the treatments. The only difference with respect to bacterial family was that the cecal abundance of Porphyromonadaceae (3.46 versus 2.11%; P = 0.073) tended to be higher for birds consuming the CC diet than those birds consuming the CT diet. Birds fed the BT diet tended to have a higher abundance of Barnesiella (0.62 versus 0.13%; P = 0.057) and a lower abundance of unclassified Ruminococcaceae (0.64 versus 1.19%; P = 0.097) than those fed the CT diet. Considering beneficial intestinal Barnesiella, this decreases and ultimately clears the colonization of vancomycin-resistant Enterococcus. The unclassified Ruminococcaceae was a low-frequency and low-abundance bacterial taxa and was not associated with intestinal pathology. CONCLUSION: These results indicate a similar modulation of cecal microbiota in laying hens by long-term feeding among transgenic CC corn, BT corn and non-transgenic corn and provide data for biosafety evaluation of the transgenic corn.

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Influence of transgenic Bt176 and non-transgenic corn silage on the structure of rumen bacterial communities

Cited by 14 publications

References 34 publications

Sequence-Based Analysis of the Intestinal Microbiota of Sows and Their Offspring Fed Genetically Modified Maize Expressing a Truncated Form of Bacillus thuringiensis Cry1Ab Protein (Bt Maize)

Sequence-Based Analysis of the Intestinal Microbiota of Sows and Their Offspring Fed Genetically Modified Maize Expressing a Truncated Form of Bacillus thuringiensis Cry1Ab Protein (Bt Maize)

High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days

Modulation of cecal microbiota in laying hens via intake of genetically modified corn with the maroACC or mCry1Ac genes

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