Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats

Santos, Nicole Ribeiro Silva; Santos, Jean Nunes dos; Reis, João José; Oliveira, Priscila Chagas; Sousa, Ana Paula Cavalcanti de; Carvalho, Carolina Montagn; Soares, Luiz Guilherme P.; Marques, Aparecida Maria Cordeiro; Pinheiro, António Luiz Barbosa

doi:10.1089/pho.2009.2500

Cited by 24 publications

(18 citation statements)

References 24 publications

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“…Diabetes mellitus (DM) is characterized by elevated blood glucose levels, an impaired blood supply, and increased production of reactive oxygen species (ROS) [1]. Chronic ROS generation is implicated in the pathogenesis of many illnesses, including atherosclerosis and inflammation [2, 3].…”

Section: 0 Introductionmentioning

confidence: 99%

“…However, the synthesis of large amounts of NO can produce nitrosative stress by reacting with superoxide to form peroxynitrite. Many authors have proposed that low level-laser therapy (LLLT) is able to influence oxidative stress parameters as well as to change the activity of antioxidant enzymes and the production of ROS [1]. Over the last decade the literature has been reported an increased interest in the application of photobiomodulation using different light sources in diseases related to increased oxidative stress, but the mechanisms involved in this response remain unclear, especially in relation to the effects of LLLT on the mitochondrial respiratory chain and on biomarkers of oxidative stress.…”

Section: 0 Introductionmentioning

confidence: 99%

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Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Tatmatsu-Rocha

Ferraresi

Hamblin

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Background and Objective Over the last decade we have seen an increased interest in the use of Low-Level Laser Therapy (LLLT) in diseases that involve increased oxidative stress. It is well established that hyperglycemia in diabetes elicits a rise in reactive oxygen species (ROS) production but the effect of LLLT remains unclear. This study aimed to investigate whether LLLT was able to improve oxidative/nitrosative stress parameters in the wound healing process in diabetic mice. Study Design/Materials and Methods Twenty male mice were divided into four groups: non-irradiated control (NIC), irradiated control (IC), non-irradiated and diabetic (NID), irradiated and diabetic (ID). Diabetes was induced by administration of streptozotocin. Wounds were created 120 days after the induction of diabetes in groups IC and ID and these groups were irradiated daily for 5 days (superpulsed 904 nm laser, average power 40 mW, 60 sec). All animals were sacrificed 1 day after the last irradiation and histology, collagen amount, catalase activity, nitrite and thiobarbituric acid reactive substances (TBARS) were measured. Results Histology showed that collagen fibers were more organized in IC and ID when compared to NID group, and significant differences in collagen content were found in group ID versus NID. Catalase activity was higher in IC group compared to other groups (p < 0.001). TBARS levels were higher in IC versus NIC, but were lower in ID versus NID (p < 0.001). Nitrite was lower in both irradiated groups versus the respective non-irradiated groups (p < 0.001). Conclusions Delayed wound healing in diabetes is still a challenge in clinical practice with high social costs. The increased production of collagen and decreased oxidative and nitrosative stress suggests that LLLT may be a viable therapeutic alternative in diabetic wound healing.

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Section: 0 Introductionmentioning

confidence: 99%

Section: 0 Introductionmentioning

confidence: 99%

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Tatmatsu-Rocha

Ferraresi

Hamblin

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…At a fluence between 3.7 and 10 J/cm 2 , splinted irradiated wounds responded better and healed quicker than nonirradiated splintered wounds. Santos et al [76] irradiated cutaneous flaps with poor circulation in diabetic Wistar rats either with 680 or 790 nm (2.5 J/cm 2 per point). It was shown that angiogenesis was increased in irradiated rats compared to control, non-irradiated rats, more so when a wavelength of 790 nm was used.…”

Section: Phototherapymentioning

confidence: 99%

Shedding Light on a New Treatment for Diabetic Wound Healing: A Review on Phototherapy

Houreld

2014

The Scientific World Journal

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Impaired wound healing is a common complication associated with diabetes with complex pathophysiological underlying mechanisms and often necessitates amputation. With the advancement in laser technology, irradiation of these wounds with low-intensity laser irradiation (LILI) or phototherapy, has shown a vast improvement in wound healing. At the correct laser parameters, LILI has shown to increase migration, viability, and proliferation of diabetic cells in vitro; there is a stimulatory effect on the mitochondria with a resulting increase in adenosine triphosphate (ATP). In addition, LILI also has an anti-inflammatory and protective effect on these cells. In light of the ever present threat of diabetic foot ulcers, infection, and amputation, new improved therapies and the fortification of wound healing research deserves better prioritization. In this review we look at the complications associated with diabetic wound healing and the effect of laser irradiation both in vitro and in vivo in diabetic wound healing.

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“…The variables used for this analysis are shown in Table 1. 13 These variables were used previously 13,21 and included inflammatory reaction, re-epithelialization, number of fibroblasts, and amount of collagen deposition and neoangiogenesis. The scoring system was based upon the number of samples with the observed grading.…”

Section: Polarized Light On Diabetic Wound Healingmentioning

confidence: 99%

Polarized Light (λ400–2000 nm) on Third-Degree Burns in Diabetic Rats: Immunohistochemical Study

Oliveira

Pinheiro²,

Reis³

et al. 2010

Photomedicine and Laser Surgery

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Influence of the Use of Laser Phototherapy (λ660 or 790 nm) on the Survival of Cutaneous Flaps on Diabetic Rats

Abstract: LPT was effective in increasing angiogenesis as seen on irradiated subjects and was more pronounced when IR laser light was used.

Cited by 24 publications

References 24 publications

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin

Shedding Light on a New Treatment for Diabetic Wound Healing: A Review on Phototherapy

Polarized Light (λ400–2000 nm) on Third-Degree Burns in Diabetic Rats: Immunohistochemical Study

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