Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Abstract

The purpose of this study was to analyze histomorphometrically the influence of the ratio of particulate autogenous bone (AB) graft/platelet-rich plasma (PRP) on bone healing in surgically created critical-size defects (CSD) in rat calvaria. Fifty rats were divided into five groups: Group C (control), Group AB, Group AB/PRP-50, Group AB/PRP-100, and Group AB/PRP-150. A 5-mm diameter critical-size defect was created in the calvarium of each animal. In Group C, the defect was filled by blood clot only. In Group AB, the defect was filled with 0.01 mL of AB graft. In Groups AB/PRP-50, AB/PRP-100, and AB/PRP-150, the defects were filled with 0.01 mL of AB graft combined with 50, 100, and 150 mL of PRP, respectively. All animals were euthanized at 30 days postoperative. Histomorphometry, using image analysis software, and histology analyses were performed. New Bone Area (NBA) and the remaining bone graft particles area (RPA) were calculated as a percentage of the total area of the original defect. Percentage data were transformed into arccosine for analysis. No defect completely regenerated with bone. Group AB/PRP-50 (41.78 AE 13.48%) had a significantly greater NBA than Groups C (19.29 AE 5.11%), AB (27.43 AE 10.90%) or AB/PRP-150 (19.17 AE 8.45%) (p < 0.05). No significant differences were observed between groups AB/PRP-50 and AB/ PRP-100 or among groups AB, AB/PRP-100, and AB/PRP-150 with regard to NBA (p > 0.05). Group AB/PRP-150 (31.59 AE 3.22%) had a significantly greater RPA than Groups AB (19.09 AE 5.21%), AB/PRP-50 (17.33 AE 4.43%), and AB/PRP-100 (19.72 AE 3.62%) (p < 0.001). No significant differences were observed among groups AB, AB/PRP-50, and AB/PRP-100 with regard to RPA (p > 0.05). The ratio AB graft/PRP influences bone healing in surgically created CSD in rat calvaria. ß

Histological and immunohistochemical evaluation of biphasic calcium phosphate and a mineral trioxide aggregate for bone healing in rat calvaria

“…Several studies 7,12,19,23,30,31 in the literature have described the tissue reactions, both in soft and hard tissues using the materials employed in this research; however, there are only a few works in the literature that compared the use of the MTA and the BCP in isolation or in association for the healing of standardized critical bone defects.…”

“…The amalgam was then applied to mark the center of the surgical wound. 23 After carefully removing the incised portion of the calvaria with the aid of a Molt dissector, the BCP was added to the wound of 20 animals (Straumann® BoneCeramic, Institut Straumann AG, Basel, Switzerland) as per the instructions of the manufacturer, filling the entire calvaria cavity (up to the edge of the defect). In 20 other samples, the MTA (MTA -Angelus®, Angelus Industry of dental products S/A, Londrina, Paraná, Brazil) was applied according to the instructions of the manufacturer.…”

Biological Evaluation of PLDLA Polymer Synthesized as Construct on Bone Tissue Engineering Application

“…During this process, the rate of PLDLA scaffold degradation was similar to that of bone formation since no signs of impaired bone growth, such as necrosis and fibrous capsule formation around the implanted material, were observed. These results indicated a good interaction at the interface between newly bone formed and the implanted PLDLA construct 33 . Although the histological analysis has revealed that the PLDLA constructs were biocompatible with bone tissue and were not cytotoxic during tissue repair, we assumed that some factors related to the porogen-leaching method used to manufacture the scaffolds may have affected the interactions between the PLDLA constructs and native tissue, thereby preventing better PLDLA scaffold-cell interaction and bone formation.…”

The Use of Platelet Rich Plasma, Bone Morphogenetic Protein-2 and Different Scaffolds in Oral and Maxillofacial Surgery - Literature Review in Comparison with Own Clinical Experience

“…From the other authors, we learned, that high concentrations of PRP produce negative effects such as suppression of proliferation of osteoblasts [46,47]. Three and a half fold to 8 fold concentrations of platelets in PRP compared to baseline seem to have the best effect.…”

“…Nagata et al [47] and Mariano et al [51] worked on a rat calvarium model using critical sized defects (CRD) of 5mm in diameter. The rat calvarium model however is established as 8 - 9 mm in literature [47,51].…”