Biological Evaluation of PLDLA Polymer Synthesized as Construct on Bone Tissue Engineering Application

Más, Bruna Antunes; Freire, Diego Coutinho de Luna; Cattani, Silvia Mara de Mello; Motta, Adriana Cristina; Barbo, Maria Lourdes Peris; Duek, Eliana Aparecida de Rezende

doi:10.1590/1980-5373-mr-2015-0559

Cited by 9 publications

(4 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been several reports of cartilage tissue repair, with TA being used as the main component of hydrogels, hydrogels surface coating agent, crosslinker of them, and finally, as a factor in preparing samples for the imaging process due to the cell fixation ability of TA. 18,296,332,339–343 In cartilage tissue repair, scaffold stability against enzymatic degradation and good mechanical properties are given priority. In this context, Natarajan et al conducted a study on the effect of polyphenols on cartilage tissue regeneration;, 296 the results showed that compounds containing TA greatly facilitated the healing process by creating thermal and enzymatic stability of the injected composite in repairing cartilage (Fig.…”

Section: Ta-based Hydrogels Applications In Tissue Engineeringmentioning

confidence: 99%

Tannic acid: a versatile polyphenol for design of biomedical hydrogels

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Ta-based Hydrogels Applications In Tissue Engineeringmentioning

confidence: 99%

Tannic acid: a versatile polyphenol for design of biomedical hydrogels

et al. 2022

View full text Add to dashboard Cite

show abstract

“…After crosslinking by ε-radiation, dimensionally stable scaffolds were obtained with up to 56% trimethylene carbonate incorporation. The fibrous mats showed Young's moduli closely matching human arteries (0.4-0.8MPa, for produce bone and fibrous tissue with poly(L-co-L,D lactic acid) 8,9 , and many others.…”

Section: Introductionmentioning

confidence: 74%

“…In brief, it has several advantages and high potential to be widely used in medical devices and biological applications. 8,10 This research aimed to produce poly(L-co-D,L lactic acid) nonwovens with intra and inter-fiber pores for biomedical applications through conventional electrospinning, and to investigate the influence of the solvent on the formation of intra-fiber pores.…”

Section: Introductionmentioning

confidence: 99%

Productionof poly(L-CO-D,L LacticAcid) porous fibers by electrospinning

Carvalho

Ciocca

Filho

et al. 2021

IJAMB

View full text Add to dashboard Cite

The production of porous scaffolds has been widely investigated by the scientific community due to its suitability for tissue engineering. Among techniques that allow the fabrication of porous materials, electrospinning is appealing for being robust and versatile. This research investigated the pore formation in poly (L-co-D,L lactic acid) fibers obtained by conventional electrospinning and the influence of chloroform as a single solvent on fiber morphology. Random and highly porous fibers with a mean diameter of 2.373 ± 0.564 µm were collected. Chloroform affects the fiber morphology, mainly for its fast evaporation and low density of charges. The solvent on the surface evaporates quickly, and the low stretch of the jet does not help the polymer to reorganize over the length of the fiber, forming pores. In conclusion, the low dielectric constant and boiling point of chloroform induce pores formation along the PLDLA fibers.

show abstract

“…Recent studies of our research group 27 showed that PLDLA scaffolds serve as support for bone tissue growth, in other words, it favors osteoblast cells activity, as well as pore size. We have tested different pore sizes and the 600 μm pore size was proved to be the most promising, once it favors proliferation, migration, and differentiation of osteoblastic cells (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Poly(L-co-D,L lactic acid-co-Trimethylene Carbonate) 3D printed scaffold cultivated with mesenchymal stem cells directed to bone reconstruction: In vitro and in vivo studies

et al. 2022

View full text Add to dashboard Cite

A recent and quite promising technique for bone tissue engineering is the 3D printing, peculiarly regarding the production of high-quality scaffolds. The 3D printed scaffold strictly provides suitable characteristics for living cells, in order to induce treatment, reconstruction and substitution of injured tissue. The purpose of this work was to evaluate the behavior of the 3D scaffold based on Poly(L-co-D,L lactic acid-co-Trimethylene Carbonate) (PLDLA-TMC), which was designed in Solidworks™ software, projected in 3D Slicer™, 3D printed in filament extrusion, cultured with mesenchymal stem cells (MSCs) and tested in vitro and in vivo models. For in vitro study, the MSCs were seeded in a PLDLA-TMC 3D scaffold with 600 μm pore size and submitted to proliferation and osteogenic differentiation. The in vivo assays implanted the PLDLA-TMC scaffolds with or without MSCs in the calvaria of Wistar rats submitted to 8 mm cranial bone defect, in periods of 8–12 weeks. The results showed that PLDLA-TMC 3D scaffolds favored adherence and cell growth, and suggests an osteoinductive activity, which means that the material itself augmented cellular differentiation. The implanted PLDLA-TMC containing MSCs, showed better results after 12 weeks prior grafting, due the absence of inflammatory processes, enlarged regeneration of bone tissue and facilitated angiogenesis. Notwithstanding, the 3D PLDLA-TMC itself implanted enriched tissue repair; the addition of cells known to upregulate tissue healing reinforce the perspectives for the PLDLA-TMC applications in the field of bone tissue engineering in clinical trials.

show abstract

Biological Evaluation of PLDLA Polymer Synthesized as Construct on Bone Tissue Engineering Application

Cited by 9 publications

References 36 publications

Tannic acid: a versatile polyphenol for design of biomedical hydrogels

Tannic acid: a versatile polyphenol for design of biomedical hydrogels

Productionof poly(L-CO-D,L LacticAcid) porous fibers by electrospinning

Poly(L-co-D,L lactic acid-co-Trimethylene Carbonate) 3D printed scaffold cultivated with mesenchymal stem cells directed to bone reconstruction: In vitro and in vivo studies

Contact Info

Product

Resources

About