Influence of the nature of the antigen on the boosting of responses to mycobacteria in M. bovis-BCG vaccinated cattle

Villarreal‐Ramos, Bernardo; Reed, Steve; McAulay, M.; Prentice, Helen; Coffey, Tracey J.; Charleston, Bryan; Howard, Chris

doi:10.1016/j.vaccine.2006.06.034

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…The Dse estimates observed for the test in M. bovis culture positive, TT positive, or IFNγ test positive animals thus varied depending on whether the samples were taken during the anamnestic window following PPDb boosting or not. The results described here are consistent with those described in the literature in relation to anamnestic serological responses following PPDb injection of infected animals 19,21,[24][25][26][27] .…”

Section: Discussionsupporting

confidence: 92%

“…such responses only appear in the late phases of the disease and have low sensitivity 9 . Several studies have shown that antibody responses are boosted following injection of tuberculin, with the optimum time for anamnestic responses to be detected occurring within a few days and lasting up to 30 days or longer post TT 19,[21][22][23][24][25][26][27] . The above studies suggest that serology tests could play a useful role in detecting and controlling bTB.…”

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confidence: 99%

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Diagnostic accuracy of the Enferplex Bovine Tuberculosis antibody test in cattle sera

O’Brien¹,

Clarke²,

Hayton³

et al. 2023

Sci Rep

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Bovine tuberculosis is a contagious bacterial disease of worldwide economic, zoonotic and welfare importance caused mainly by Mycobacterium bovis infection. Current regulatory diagnostic methods lack sensitivity and require improvement. We have developed a multiplex serological test for bovine tuberculosis and here we provide an estimate of the diagnostic accuracy of the test in cattle. Positive and negative reference serum samples were obtained from animals from Europe and the United States of America. The diagnostic specificity estimate was 98.4% and 99.7% using high sensitivity and high specificity settings of the test respectively. Tuberculin boosting did not affect the overall specificity estimate. The diagnostic sensitivity in samples from Mycobacterium bovis culture positive animals following tuberculin boosting was 93.9%.The relative sensitivity following boosting in tuberculin test positive, lesion positive animals and interferon gamma test positive, lesion positive animals was 97.2% and 96.9% respectively. In tuberculin test negative, lesion positive animals and in interferon gamma test negative, lesion positive animals, the relative sensitivity following tuberculin boosting was 88.2% and 83.6% respectively. The results show that the test has high diagnostic sensitivity and specificity and can detect infected animals that are missed by tuberculin and interferon gamma testing.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 99%

Diagnostic accuracy of the Enferplex Bovine Tuberculosis antibody test in cattle sera

O’Brien¹,

Clarke²,

Hayton³

et al. 2023

Sci Rep

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“…Presumptive identifications are based on data provided by the Mascot search results and previously performed 2DE analysis of Mycobacterium spp. CFPs [15,20,27,28,32,35,40]. …”

Section: Resultsmentioning

confidence: 99%

“…Although this number falls well short of the 800 plus protein spots reported to exist in M. bovis culture filtrate [12], the MASS-SPEC results from this study indicated that each protein spot actually contained a mixture of multiple M. bovis CFPs (TABLE 1). Another potential reason for this difference in detected protein spots is that our preparation of M. bovis CFPs was obtained from 9-week-old culture supernatant in order to characterize the actual constituents that make up PPD tuberculin whereas other studies used shorter culture times for their CFP preparations [12,35]. The concentration of CFPs changes as the culture time increases, with the more stable proteins, for example MPB70/83, building up over time while others, such as MPB64 tend to degrade in the culture supernatant [14].…”

Section: Discussionmentioning

confidence: 99%

“…While several other studies have indicated a similar boosting effect caused by tuberculin for both the humoral [1,9,20,44,45] and cellular [1,19,46] immune responses of M. bovis infected cattle, the precise mechanisms by which this occurs remains to be determined [35]. Harboe et al [43] originally hypothesized that the marked increase in antibody response following skin testing in cattle was principally due to the presence of native MPB70 in M. bovis PPD tuberculin.…”

Section: Discussionmentioning

confidence: 99%

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Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle

2010

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BackgroundBovine tuberculosis, caused by Mycobacterium bovis, afflicts approximately 50 million cattle worldwide and is detected by the tuberculin skin test (TST). While it has long been recognized that purified protein derivative (PPD) tuberculin is composed of a mixture of M. bovis derived protein components, little is known about the quality, relative quantity and identity of the proteins that make up PPD tuberculin. We manufactured a sterile filtered PPD tuberculin (SF-PPD) from a nine-week-old M. bovis culture supernatant in order to characterise the culture filtrate proteins (CFP) which make up M. bovis PPD tuberculin and to compare the antibody response of M. bovis infected versus M. bovis sensitized cattle.ResultsSF-PPD resolved into approximately 200 discrete spots using two-dimensional polyacrylamide gel electrophoresis (2-DE) while fewer than 65 spots could be discerned from 2-DE gels of tuberculin derived from autoclaved culture supernatant. Two dimensional Western blot analyses indicated that sera from M. bovis sensitized cattle recognized additional SF-PPD antigens as compared to M. bovis infected cattle at seven weeks post infection/sensitization. However, application of a comparative tuberculin skin test resulted in an antibody boosting response to the same set of M. bovis CFPs in both the M. bovis infected and M. bovis sensitized cattle.ConclusionsWe concluded that it is the heat sterilization of the M. bovis CFPs that causes severe structural changes to the M. bovis proteins. This work suggests that M. bovis infected cattle and cattle artificially sensitized to M. bovis with an injection of heat killed cells exhibit similar antibody responses to M. bovis antigens.

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The antigen-specific memory CD8+ T-cell response induced by BCG in cattle resides in the CD8+γ/δTCR−CD45RO+ T-cell population

Hogg

Worth

Beverley

et al. 2009

Vaccine

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Influence of the nature of the antigen on the boosting of responses to mycobacteria in M. bovis-BCG vaccinated cattle

Cited by 9 publications

References 24 publications

Diagnostic accuracy of the Enferplex Bovine Tuberculosis antibody test in cattle sera

Diagnostic accuracy of the Enferplex Bovine Tuberculosis antibody test in cattle sera

Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle

The antigen-specific memory CD8+ T-cell response induced by BCG in cattle resides in the CD8+γ/δTCR−CD45RO+ T-cell population

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