Influence of the human paraoxonase polymorphism (PON1 192) on the carotid-wall thickening in a healthy population

Dessı̀, Mariarita; Gnasso, Agostino; Motti, Corradino; Pujia, Arturo; Irace, Concetta; Casciani, Stefania; Staffa, Francesco; Federici, Giorgio; Cortese, Claudio

doi:10.1097/00019501-199912000-00008

Cited by 23 publications

(8 citation statements)

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“…Gnasso et al [26] have reported a significant association between the PON1 192R allele and the presence of carotid atherosclerosis in subjects with high HDL levels, whereas a lack of association was observed in those who had low HDL levels. Dessi et al [27] have found no association between common carotid IMT and the PON1 192 polymorphism in healthy population. Similar findings were reported in French subjects with type 2 diabetes mellitus [28].…”

Section: Discussionmentioning

confidence: 93%

The paraoxonase promoter polymorphism (−107)T>C is not associated with carotid intima-media thickness in Sicilian hypercholesterolemic patients

Campo

2004

Clinical Biochemistry

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Background and Objectives: Increased plasma low-density lipoprotein-cholesterol (LDL-C) levels in hypercholesterolemic subjects are associated with enhanced LDL oxidation that represents an additional risk for atherosclerotic disease. Human serum paraoxonase (PON1), a high-density lipoprotein (HDL) associated enzyme, has been shown to protect LDL from oxidation, thus playing an important role in reducing the risk of atherosclerosis. PON1 gene polymorphisms have been found to be associated with the variations in serum PON1 levels and activities, and with the risk for coronary artery disease (CAD). This study was performed to evaluate the contribution of the PON1 promoter (À107)T>C and the coding region Gln 192 Arg (Q192R) and Leu 55 Met (L55M) polymorphisms to the presence of carotid atherosclerosis in 208 Sicilian subjects with primary hypercholesterolemia.Methods: Carotid artery intima-media wall thickness (IMT) was measured as an indicator of early atherosclerotic disease. The subjects were classified according to whether they have a normal (V1 mm) or an abnormal (>1 mm) IMT. Subjects were also investigated for physical and biochemical parameters, including PON1 activity.Results: No significant differences were detected among the PON1 genotypes with respect to age, sex, BMI, plasma lipids, systolic blood pressure in both groups of patients. There were significant differences between PON1 genotypes with respect to PON1 activity. The 192QQ, 55MM and (À107)TT genotypes showed lower PON1 activity compared to the RR, LL and CC genotypes. The PON1 (À107)T>C genotype distribution in both IMT groups showed no significant differences in percentage of TT, CT and CC genotypes. Similar results were obtained analyzing the Q192R and L55M genotype frequencies.Stepwise forward logistic regression analysis confirmed the lack of association between PON1 genotypes and carotid abnormalities.Conclusions: In conclusion, our data provided no evidence of a significant association between either PON1 promoter (À107)T>C or coding region, Q192R and L55M, polymorphisms and early carotid atherosclerosis in Sicilian hypercholesterolemic subjects.

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Section: Discussionmentioning

confidence: 93%

The paraoxonase promoter polymorphism (−107)T>C is not associated with carotid intima-media thickness in Sicilian hypercholesterolemic patients

Campo

2004

Clinical Biochemistry

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“…Several studies have examined PON1 gene variants with respect to IMT, with some showing an association [11, 14, 16, 17, 29], and others failing to find an effect of different alleles on IMT [10, 12, 13, 15, 30, 31]. Because of the conflicting results the role of the PON1 gene in carotid artery disease is currently unclear.…”

Section: Discussionmentioning

confidence: 99%

Association between Human Paraoxonase 1 Activity and Intima-Media Thickness in Subjects under 55 Years of Age with Carotid Artery Disease

et al. 2007

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Background: Human serum paraoxonase (PON1) protects lipoproteins against oxidation by hydrolyzing lipid peroxides in oxidized low-density lipoprotein (oxLDL); therefore, it may protect against atherosclerosis. PON1 activity and polymorphisms have been inconsistently associated with carotid artery disease. The goal of this study was to clarify the role of PON1 activity and phenotype on carotid artery disease and its correlation with some inflammatory and immune markers in subjects under 55 years with early-onset carotid atherosclerosis. Methods: Sixty patients with occlusive carotid artery disease and 30 healthy controls were enrolled. Intima-media thickness (IMT) was measured by high-resolution ultrasound of both common carotid arteries. Anti-oxLDL antibody levels were determined by ELISA. Results: In the whole study population we found a negative correlation between PON1 activity and IMT (r = –0.27, p = 0.011), and between salt-stimulated PON1 activity and IMT (r = –0.24, p = 0.02). Both PON1 activity and salt-stimulated PON1 activity negatively correlated with anti-oxLDL levels (r = –0.28, p = 0.008; r = –0.26, p = 0.01). PON1 activity was lower in patients compared to controls; however, the difference was not significant.PON1 phenotype distribution of patients and controls did not differ significantly. Conclusion: The importance of PON1 activity as a predictive risk factor for early-onset occlusive carotid artery disease should be assessed in future studies.

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“…To perform this task, HDLs interact in the bloodstream with other lipoproteins, exchange cholesterol and triglyceride with them, ensuring the transport of excess cholesterol to the liver [18]. Furthermore, HDLs also protect LDLs from oxidation, due to their content in paraoxonase [6,11]. Taken together, the plasma lipoproteins are in dynamic equilibrium and the concentration (and activity) of HDL may modify the composition and structure of the other lipoproteins.…”

Section: Discussionmentioning

confidence: 99%

The effect of HDL cholesterol on blood and plasma viscosity in healthy subjects

Carallo

Irace

Franceschi

et al. 2013

Clinical Hemorheology and Microcirculation

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OBJECTIVE: The influence of lipids on blood and plasma viscosity has not been fully elucidated. In this study we evaluated the contribution of HDL cholesterol to blood and plasma viscosity, in healthy subjects. METHODS: One hundred and forty-four (80 males and 64 females) subjects were enrolled among free-living participants to a cardiovascular disease screening. Exclusion criteria were: diabetes, elevated triglycerides, elevated LDL cholesterol, smoking and drug use. Blood lipids were measured by routine methods. Blood and plasma viscosity were measured by a cone-plate viscometer (Wells-Brookfield DV-III, Stoughton, U.S.A.). Subjects were divided in two groups: at low (<50 mg/dl) and high HDL cholesterol (>50 mg/dl). RESULTS: Blood and plasma viscosity were similar in subjects at low and high HDL cholesterol. In univariate analysis none of the lipid variables was significantly correlated with blood and/or plasma viscosity. In multivariate analysis only LDL cholesterol was marginally associated with blood viscosity. CONCLUSION: HDL cholesterol does not influence blood and plasma viscosity in healthy normolipidemic subjects. LDL cholesterol is marginally associated with blood viscosity. C. Carallo et al. / HDL cholesterol, blood and plasma viscosityIn this study we evaluated the contribution of HDL cholesterol to blood and plasma viscosity, in healthy subjects with normal lipid levels. Subjects and methods SubjectsHealthy subjects were enrolled among free-living participants in a cardiovascular disease prevention program [10,14]. Smoking, diabetes, plasma triglycerides above 150 mg/dl, LDL cholesterol above 130 mg/dl, and drug use were exclusion criteria for the present analysis.Subjects were divided in two groups: those with low HDL cholesterol (≤50 mg/dl) and those with high HDL cholesterol (>50 mg/dl).The Ethics Committee of the Azienda Policlinico Mater Domini approved the study. All recruited subjects gave their informed consent.Moreover, the present paper was developed in respect for the ethical guidelines of Clinical Hemorheology and Microcirculation.

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Influence of the human paraoxonase polymorphism (PON1 192) on the carotid-wall thickening in a healthy population

Cited by 23 publications

References 0 publications

The paraoxonase promoter polymorphism (−107)T>C is not associated with carotid intima-media thickness in Sicilian hypercholesterolemic patients

The paraoxonase promoter polymorphism (−107)T>C is not associated with carotid intima-media thickness in Sicilian hypercholesterolemic patients

Association between Human Paraoxonase 1 Activity and Intima-Media Thickness in Subjects under 55 Years of Age with Carotid Artery Disease

The effect of HDL cholesterol on blood and plasma viscosity in healthy subjects

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