Influence of the glucose concentration on the yield of biomass and lipopolysaccharide in Salmonella cultures

Schlecht, S; Galanos, Chris

doi:10.1016/s0934-8840(11)80634-1

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Classification of isolates into rough (no O-chain) and smooth phenotypes was done by slide agglutination with antiserum specific for group D1 isolates producing tyvelose (factor 9) and glucosylated O-chain (factor 12) (Difco). Isolates 19,38,39,40,41, and 46 were grown in BHI broth supplemented with 10 mM glucose, as recommended for recovery of LPS, while isolates 7, 25, 27, 30, 37, 58, and 46 were supplemented with 10 mM N-acetylglucosamine (GlcNAc) (32). By choosing a class of carbohydrate different from that usually used for supplementation, we introduced a source of variation that would help us assess more accurately the amount of sample variation that could be expected as different laboratories processed different isolates.…”

Section: Methodsmentioning

confidence: 99%

Clinical and Veterinary Isolates of Salmonella enterica Serovar Enteritidis Defective in Lipopolysaccharide O-Chain Polymerization

Guard-Petter

Parker

Asokan

et al. 1999

Appl Environ Microbiol

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Twelve human and chicken isolates of Salmonella enterica serovar Enteritidis belonging to phage types 4, 8, 13a, and 23 were characterized for variability in lipopolysaccharide (LPS) composition. Isolates were differentiated into two groups, i.e., those that lacked immunoreactive O-chain, termed rough isolates, and those that had immunoreactive O-chain, termed smooth isolates. Isolates within these groups could be further differentiated by LPS compositional differences as detected by gel electrophoresis and gas liquid chromatography of samples extracted with water, which yielded significantly more LPS in comparison to phenol-chloroform extraction. The rough isolates were of two types, the O-antigen synthesis mutants and the O-antigen polymerization (wzy) mutants. Smooth isolates were also of two types, one producing low-molecular-weight (LMW) LPS and the other producing high-molecular-weight (HMW) LPS. To determine the genetic basis for the O-chain variability of the smooth isolates, we analyzed the effects of a null mutation in the O-chain length determinant gene, wzz (cld) of serovar Typhimurium. This mutation results in a loss of HMW LPS; however, the LMW LPS of this mutant was longer and more glucosylated than that from clinical isolates of serovar Enteritidis. Cluster analysis of these data and of those from two previously characterized isogenic strains of serovar Enteritidis that had different virulence attributes indicated that glucosylation of HMW LPS (via oafR function) is variable and results in two types of HMW structures, one that is highly glucosylated and one that is minimally glucosylated. These results strongly indicate that naturally occurring variability inwzy, wzz, and oafR function can be used to subtype isolates of serovar Enteritidis during epidemiological investigations.

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Section: Methodsmentioning

confidence: 99%

Clinical and Veterinary Isolates of Salmonella enterica Serovar Enteritidis Defective in Lipopolysaccharide O-Chain Polymerization

Guard-Petter

Parker

Asokan

et al. 1999

Appl Environ Microbiol

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Culture and biological activity ofPropionibacterium acnes

1997

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Administration of killed Propionibacterium acnes to experimental animals leads to the development of hypersensitivity to the lethal and cytokine-inducing effects of endotoxin. This sensitizing property of P. acnes is not always expressed by different bacterial preparations. Its expression depends very much on the conditions employed for the cultivation of this microorganism. The present study investigates which culturing conditions result in P. acnes preparations with optimal sensitizing properties. The composition of the medium, the culturing time and temperature as well as the type of cultivation (in minifermentor or stationary culture) were all varied for this purpose. The resulting bacterial preparations were killed at 65 degrees C for 1 h and tested for sensitizing activity. The results show that stationary cultures of P. acnes grown at 37 degrees C for 4 to 5 days in the appropriate medium produce biologically active preparations with satisfactory sensitizing activity.

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Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology

Henriques

Jessouroun

Lima

et al. 2006

Process Biochemistry

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Influence of the glucose concentration on the yield of biomass and lipopolysaccharide in Salmonella cultures

Cited by 3 publications

References 17 publications

Clinical and Veterinary Isolates of Salmonella enterica Serovar Enteritidis Defective in Lipopolysaccharide O-Chain Polymerization

Clinical and Veterinary Isolates of Salmonella enterica Serovar Enteritidis Defective in Lipopolysaccharide O-Chain Polymerization

Culture and biological activity ofPropionibacterium acnes

Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology

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