Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology

Henriques, A.W.S.; Jessouroun, Ellen; Lima, Edson L. S.; Alves, Tito Lívio Moitinho

doi:10.1016/j.procbio.2006.03.044

Cited by 9 publications

(7 citation statements)

References 30 publications

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“…carbohydrates) for respiration and a concomitant decrease in cell weight (Vonshak et al 1982). Optimum temperature for polysaccharide production in other microorganisms studied were 37°C in Neisseria meningitides and Aspergillus carneus (Henriques et al 2006;Kaushik et al 2006), 31°C in Sphingomonas paucimobilis (West 2003), 30°C in Aureobasidium pullulans (Cheng et al 2009) and 25°C in Phellinus linteus (Kim et al 2001). The variance analysis by student ''t'' test presented significantly differences at a level of 1% for the temperatures tested.…”

Section: Optimization Of Temperaturementioning

confidence: 99%

“…Optimum pH for polysaccharide production in other microorganisms studied were pH 7 in Pseudomonas fluorescen (with slightly thicker biofilms when there is no pH control), Arthrobacter viscosus (grown in flask), P. aeruginosa, Micrococcus sp. and Neisseria meningitidis (Oliveira et al 1994;Lopez et al 2003;Henriques et al 2006;Kiliç and Dönmez 2008), and pH 8 in Arthrobacter viscosus (grown in bioreactor) and Ochrobactrum sp. (Lopez et al 2003;Kiliç and Dönmez 2008).…”

Section: Optimization Of Temperaturementioning

confidence: 99%

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Screening, production, optimization and characterization of cyanobacterial polysaccharide

Singh

Das

2011

World J Microbiol Biotechnol

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Biotechnological applications of algal polysaccharide as emulsifiers, thickeners and laxatives have led to the screening and selection of certain diazotrophic filamentous cyanobacteria from saline/alkaline soil of Madhya Pradesh, India. Strain specific variation in cell bound, extracellular and total polysaccharide content was quantified under laboratory conditions. Among the cyanobacterial isolates examined Nostoc calcicola RDU-3 was found to produce highest amount (105 mg l -1 ) of extracellular polysaccharide on 44th day of growth under diazotrophic growth conditions. Extracellular polysaccharide production of cyanobacterium Nostoc calcicola RDU-3 was optimal at pH-10, temperature 35°C, photoperiod of 24 h and in white light. The Gas Chromatographic analysis of polysaccharide from Nostoc calcicola RDU-3 revealed the presence of ribose (36.03%), xylose (34.13%), rhamnose (29.67%) and glucose (4.0%). The polysaccharide is novel in that it possesses ribose as the predominant monosaccharide with very low levels of glucose. Predominance of ribose monosaccharide is the unique feature which is reported to be used as metabolic supplement to the heart. IR spectrum of extracellular polysaccharide revealed the presence of sulphate group. Such sulphated polysaccharide is reported to have antiviral properties.

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Section: Optimization Of Temperaturementioning

confidence: 99%

Section: Optimization Of Temperaturementioning

confidence: 99%

Screening, production, optimization and characterization of cyanobacterial polysaccharide

Singh

Das

2011

World J Microbiol Biotechnol

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“…These types of models require several sets of data for validation and their predictive ability is unreliable under extrapolation conditions, so they are usually applied to well-established processes where data is readily available or for batch-to-batch iterative optimisation (Teixeira et al, 2006). Therefore, empirical models with secondary order polynomials have been widely applied to study the effect of variables on cell growth and optimisation of bioprocesses (Henriques et al, 2006;Nikerel et al, 2006;Rita et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Modelling the industrial production of vinegar in aerated-stirred fermentors in terms of process variables

González-Sáiz

Garrido-Vidal

Pizarro

2009

Journal of Food Engineering

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“…Conventional vaccines based on meningococcal polysaccharide elicit an immune response against the capsular polysaccharide [ 15 ]. Nevertheless, the use of purified PS vaccines has been limited due to the poor immunogenicity in newborn and young children as well as the relative short duration of induced immunity [ 16 ]. These capsules are considered T-cell-independent antigens, so their use in vaccines has been optimal only with protein-carrier chemical conjugation to form PS-conjugate vaccines, which are polysaccharide-protein hybrids formed by covalent attachment of a protein to a bacterial PS [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

Baruque-Ramos

Arauz

Paz

et al. 2016

CBE

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Serogroup C polysaccharide from Neisseria meningitidis (PS) constitutes the antigen for the respective vaccine production. In order to investigate the enhancement of the final PS concentration (Pf), as well as the overall yield factor (PS/biomass) (YP/X), 13 total cultivations distributed in 6 series (from A to F) were carried out in Frantz medium (40 L plus inoculum) in a 80L bioreactor at 35oC, 0.4 atm, 120 rpm, airflow rate of 5 L/min and KLa = 4.2 h-1. The series (A-F) correspond to different experimental conditions as follows: A) without pH and dissolved O2 controls; B) pH control at 6.5; C) pH control at 6.5 and glucose pulse at the 10th hour; D) dissolved O2 control at 10% saturation value; E) pH control at 7.4; F) dissolved O2 limitation (set rotation at 55 rpm). Concentrations of dry biomass, PS, cellular nitrogen, residual glucose, organic and inorganic nitrogen in the medium were measured. The best results were represented by series A (averages of Pf = 0.15 g/L and YP/X = 107 mg/g). The presented findings could be useful for a proper Frantz medium reformulation in order to obtain a greater amount of PS and improve the vaccine development in industrial scale-up production.

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Capsular polysaccharide production by Neisseria meningitidis serogroup C: Optimization of process variables using response surface methodology

Cited by 9 publications

References 30 publications

Screening, production, optimization and characterization of cyanobacterial polysaccharide

Screening, production, optimization and characterization of cyanobacterial polysaccharide

Modelling the industrial production of vinegar in aerated-stirred fermentors in terms of process variables

Polysaccharide Production in Pilot Scale Bioreactor Cultivations of Neisseria meningitidis Serogroup C

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