Influence of the Culture Medium on the Production of Iturin A by Bacillus subtilis

Abstract

The production of iturin A by Bacillus subtilis was studied with respect to the composition of the culture medium. Increasing phosphate concentrations did not modify the antibiotic yield. Fructose, sucrose and mannitol were better carbon sources than glucose for antibiotic production. The nature of the nitrogen source was an important factor in the production of antibiotic. Among the amino acids which are components of iturin A, L-asparagine was the best substrate for the biosynthesis of iturin A; L-glutamine and L-serine were rather poor substrates while L-proline and D-tyrosine gave no antibiotic. Ammonium salts permitted good synthesis of antibiotic but the addition of calcium ions to the culture medium inhibited the excretion of antibiotic from the cells.

Isolation and identification of a potential biocontrol agent Bacillus subtilis QM3 from Qinghai yak dung in China

“…This was in agreement with Besson et al (1987), who reported mannitol, fructose and sucrose as better carbon sources than glucose for the synthesis of iturin A in B. subtilis. There is a regulatory mechanism called catabolite repression by which the cell coordinated the metabolism of the carbon and energy source, to maximize the efficiency of nutrient utilization and control other metabolic processes (Bernal et al 2002).…”

The response of Bacillus subtilis ATCC 21332 to manganese during continuous-phased growth

“…Production of antibiotics by B. subtilis is part of a complex response to changes in the nutrient environment, and is usually associated with the stationary phase of the batch growth cycle (Nakano et al 1988;Chevanet et al 1986;Besson et al 1987). Previous work with strain ATCC 21332 has indicated that surfactin production occurs during the exponential phase of batch growth (Cooper et al 1981 ;Sheppard and Mulligan 1987) but increases during transition to the stationary phase (Vater 1986).…”

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

“…Pathak (2011) and Usama (2003) observed starch and lactose as the ideal carbon sources respectively. Besson et al, 1987 studied the influence of growth media on production of iturin A by B. subtilis using glucose as carbon source. Mizumoto et al, 2007 showed addition of glucose as carbon source in minimal salt medium containing Okra enhanced the bioactive iturin A production in solid state fermentation (SSF) by B. subtilis RB14-CS.…”