Influence of the association between platelet-rich fibrin and bovine bone on bone regeneration. A histomorphometric study in the calvaria of rats

Oliveira, Marina Reis; Silva, A. deC.; Ferreira, Sueli Mara Soares Pinto; Avelino, Cassia Carneiro; Garcia, Idelmo Rangel; Mariano, Ronaldo Célio

doi:10.1016/j.ijom.2014.12.005

Cited by 55 publications

(56 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Griffin et al showed in a systematic review that although early clinical results suggest that the use of PRP is safe and feasible, however presents with no clinical benefit in either acute or delayed fracture healing was observed and therefore its use in bone regeneration was not undetermined [61]. While other models have also shown favorable results on new bone formation with platelet concentrates [63–65], the results from our study showed that PRP had little influence on osteoblast differentiation (Fig. 5).…”

Section: Discussionmentioning

confidence: 99%

Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour

et al. 2017

View full text Add to dashboard Cite

BackgroundThe use of platelet rich plasma (PRP, GLO) has been used as an adjunct to various regenerative dental procedures. The aim of the present study was to characterize the influence of PRP on human gingival fibroblasts, periodontal ligament (PDL) cells and osteoblast cell behavior in vitro.MethodsHuman gingival fibroblasts, PDL cells and osteoblasts were cultured with conditioned media from PRP and investigated for cell migration, proliferation and collagen1 (COL1) immunostaining. Furthermore, gingival fibroblasts were tested for genes encoding TGF-β, PDGF and COL1a whereas PDL cells and osteoblasts were additionally tested for alkaline phosphatase (ALP) activity, alizarin red staining and mRNA levels of osteoblast differentiation markers including Runx2, COL1a2, ALP and osteocalcin (OCN).ResultsIt was first found that PRP significantly increased cell migration of all cells up to 4 fold. Furthermore, PRP increased cell proliferation at 3 and 5 days of gingival fibroblasts, and at 3 days for PDL cells, whereas no effect was observed on osteoblasts. Gingival fibroblasts cultured with PRP increased TGF-β, PDGF-B and COL1 mRNA levels at 7 days and further increased over 3-fold COL1 staining at 14 days. PDL cells cultured with PRP increased Runx2 mRNA levels but significantly down-regulated OCN mRNA levels at 3 days. No differences in COL1 staining or ALP staining were observed in PDL cells. Furthermore, PRP decreased mineralization of PDL cells at 14 days post seeding as assessed by alizarin red staining. In osteoblasts, PRP increased COL1 staining at 14 days, increased COL1 and ALP at 3 days, as well as increased ALP staining at 14 days. No significant differences were observed for alizarin red staining of osteoblasts following culture with PRP.ConclusionsThe results demonstrate that PRP promoted gingival fibroblast migration, proliferation and mRNA expression of pro-wound healing molecules. While PRP induced PDL cells and osteoblast migration and proliferation, it tended to have little to no effect on osteoblast differentiation. Therefore, while the effects seem to favor soft tissue regeneration, the additional effects of PRP on hard tissue formation of PDL cells and osteoblasts could not be fully confirmed in the present in vitro culture system.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour

et al. 2017

View full text Add to dashboard Cite

show abstract

“…However, in another set of experiments, it was found that PRP had significant beneficial effects on improving the rate of wound healing and fat graft survival rates and in enhancing bone graft regeneration [6,7]. The application of PRP with Biop-Oss onto bone defects resulted in an increase in granulation, but only when associated with Bio-Oss [3]. Finally, the influences of PRP on cells in vitro were examined to see whether its influences are indicative of those required for wound healing in vivo [3].…”

Section: General Introductionmentioning

confidence: 99%

“…The application of PRP with Biop-Oss onto bone defects resulted in an increase in granulation, but only when associated with Bio-Oss [3]. Finally, the influences of PRP on cells in vitro were examined to see whether its influences are indicative of those required for wound healing in vivo [3].…”

Section: General Introductionmentioning

confidence: 99%

“…Although these studies were comprehensive, their results were often conflicting, claiming that PRP induces significant influences, may induce some improvements, or induced no improvements. Similarly, there was no consistency in the outcomes of studies for the influence of PRP on orthopedic bone and soft tissue injuries although the evidence suggested a small trend favoring the effectiveness of PRP [1][2][3][4][5]. However, in another set of experiments, it was found that PRP had significant beneficial effects on improving the rate of wound healing and fat graft survival rates and in enhancing bone graft regeneration [6,7].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

Kuffler

2015

Mol Neurobiol

View full text Add to dashboard Cite

Platelet-rich plasma (PRP) has been tested in vitro, in animal models, and clinically for its efficacy in enhancing the rate of wound healing, reducing pain associated with injuries, and promoting axon regeneration. Although extensive data indicate that PRP-released factors induce these effects, the claims are often weakened because many studies were not rigorous or controlled, the data were limited, and other studies yielded contrary results. Critical to assessing whether PRP is effective are the large number of variables in these studies, including the method of PRP preparation, which influences the composition of PRP; type of application; type of wounds; target tissues; and diverse animal models and clinical studies. All these variables raise the question of whether one can anticipate consistent influences and raise the possibility that most of the results are correct under the circumstances where PRP was tested. This review examines evidence on the potential influences of PRP and whether PRP-released factors could induce the reported influences and concludes that the preponderance of evidence suggests that PRP has the capacity to induce all the claimed influences, although this position cannot be definitively argued. Well-defined and rigorously controlled studies of the potential influences of PRP are required in which PRP is isolated and applied using consistent techniques, protocols, and models. Finally, it is concluded that, because of the purported benefits of PRP administration and the lack of adverse events, further animal and clinical studies should be performed to explore the potential influences of PRP.

show abstract

“…Also, when adding PRF to an alloplastic graft (HA/β‐ TCP) or autologous graft, a significantly increased regeneration of calvarial defects was observed both at 4 and 8 weeks compared to the use of PRF or the grafts alone (Acar, Yolcu, Gul, Keles & Erdem, ; Pripatnanont, Nuntanaranont, Vongvatcharanon & Phurisat, ). Conflicting results were reported when combining PRF to DBBM (Oliveira, deC Silva, Ferreira, Avelino & Garcia, ; Pripatnanont et al., ; Yoon, Lee & Yoon, ). Similar regeneration outcomes were observed when rat calvarial CSDs were treated with PRF or demineralized bone matrix (Sindel, Dereci, Toru & Tozoglu, ).…”

Section: Resultsmentioning

confidence: 98%

The use of bioactive factors to enhance bone regeneration: A narrative review

Donos

Dereka

Calciolari

2019

J Clinic Periodontology

View full text Add to dashboard Cite

Aim This review critically appraises the available knowledge on the pre‐clinical and clinical use of bioactive factors for bone regeneration in the cranial and maxillofacial area. Materials and Methods The use of growth factors, amelogenins and autologous platelet concentrates (APCs) for bone regeneration was reviewed in a systematic manner. More specifically, pre‐clinical and clinical studies on ridge preservation, alveolar ridge augmentation, regeneration of peri‐implant defects and sinus augmentation models were considered. Results Amongst different bioactive factors, the highest pre‐clinical and clinical evidence of a positive effect on bone formation is associated with rhBMP‐2 and the lowest with amelogenins. While APCs seem to accelerate clinical healing and reduce postoperative discomfort, there is insufficient and contrasting evidence of a significant effect on hard tissue regeneration for the different clinical applications. Conclusions Although there is increasing evidence that bioactive factors might enhance the bone regeneration process, the great heterogeneity of the available studies and the limited number of RCTs do not allow to draw robust conclusions. Issues that still need to be investigated include the optimal carriers for bioactive agents (direct vs. indirect), the dosage, the timing of administration, as well as the possibility of combining different agents to promote synergistic effects.

show abstract

Influence of the association between platelet-rich fibrin and bovine bone on bone regeneration. A histomorphometric study in the calvaria of rats

Cited by 55 publications

References 24 publications

Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour

Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour

Platelet-Rich Plasma Promotes Axon Regeneration, Wound Healing, and Pain Reduction: Fact or Fiction

The use of bioactive factors to enhance bone regeneration: A narrative review

Contact Info

Product

Resources

About