Influence of temperature on the kinetics of adsorption and desorption of clavulanic acid by ionic exchange

Barboza, Marlei; Almeida, Renata Maria Rosas Garcia; Hokka, Carlos O.

doi:10.1016/s1369-703x(02)00103-1

Cited by 31 publications

(34 citation statements)

References 6 publications

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“…Further purification is achieved by anion exchange chromatography and, owing to the unstable nature of the free acid, the CA is isolated as lithium, potassium or sodium salt. Purification of CA by anion exchange resin is also discussed in the literature [2,3]. However, the authors did not succeed in concentrating the solution.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

Silva

Bovarotti

Rodrigues

et al. 2008

Bioprocess Biosyst Eng

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The purification of clavulanic acid (CA), which is an important beta-lactam antibiotic produced by submerged cultivation of Streptomyces clavuligerus, was studied through the use of phosphate and polyethylene glycol-based aqueous two-phase systems. The parameters' effect on the yield and purification was evaluated through an experimental design and the preliminary results showed that the polyethylene molecular mass and tie-line length and phase volume ratio exerted the strongest effect on the yield and distribution coefficient in the range tested. In addition, the response surface methodology was used to optimize the distribution coefficient, yield, and purification factor. The optimal conditions of yield and purification factor are in the regions where polyethylene has a low molecular mass, pH close to the isoelectric point, and lower top phase volume. A 100% yield and a 1.5-fold purification factor are obtained when extracting CA by maximizing the conditions of an aqueous two-phase system.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

Silva

Bovarotti

Rodrigues

et al. 2008

Bioprocess Biosyst Eng

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“…In the case of CA, the reported yields for ion exchange adsorption and liquid-liquid operations are generally low [5]. In fact, this extremely unstable structure undergoes rapid degradation under normal processing conditions [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of kinetic and mass transfer parameters for adsorption of clavulanic acid into natural and synthetic zeolites

Forte

Mazutti

Filho

et al. 2011

Biotechnol Bioproc E

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This work investigates the adsorption of clavulanic acid using natural six cationic forms (Na + , Ca +2 , Ba +2 , Sr 2+ , K + , and Mg 2+ ) of the X and NZ zeolites in a stirred tank reactor since the separation is an important step of the biomolecule production. A mathematical model was proposed taking into account the transport of CA molecules from the liquid phase to the surface of the adsorbent and after diffusion into the particles. The estimated kinetic and mass transfer parameters were used to evaluate adsorption rates and mass transfer resistances involved in the separation of clavulanic acid from the broth. It has been shown that mass-transfer phenomena were a limiting step in the clavulanic acid adsorption process and that the adsorption rate should be considered to evaluate the system. Amongst the materials, the synthetic zeolite NaX was selected as the most appropriate material to separate clavulanic acid because this material presented the highest values for the observed reaction rate, compensating for the external mass transfer resistance. Modeling and simulation of clavulanic acid purification using the zeolite NaX showed a satisfactory fitting of experimental data. The model was used to simulate the process and it was evaluated for its technical and economical viability by comparisons considering the influence of the solid:liquid ratio on the adsorption equilibrium time and on the hydrolysed mass of biomolecule.

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“…This technique has developed rapidly in recent decades mainly since the introduction of synthetic adsorbers [3]. As a chromatographic method alternative to solvent extraction, other groups applied ion exchange chromatography (IEC) for KCA separation [4][5][6][7]. However, KCA has no strong hydrophobic group and exhibits low chemical stability.…”

Section: Introductionmentioning

confidence: 99%

Recovery of potassium clavulanate from fermentation broth by ion exchange chromatography and desalting electrodialysis

Kim

Kang

Chang

2009

Biotechnol Bioproc E

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Ion exchange chromatography (IEC) and desalting electrodialysis (DSED) processes were developed for the recovery and purification of potassium clavulanate (KCA) from fermentation broth. A strong anion exchanger, Amberlite IRA 400 resin, a potassium acetate solution as equilibrium buffer, and a potassium chloride (KCl) solution as elution buffer were used for the recovery of KCA in IEC. In order to determine optimal operating conditions, the effects of various operating parameters such as equilibrium buffer pH and concentration, elution buffer concentration, gradient length, and volumetric flow rate on KCA recovery and by-product removal were investigated using a simulated fermentation broth. In the subsequent step of DSED, employing cation (Neocepta CMS, Tokuyama, Japan) and anion (Neocepta ACS, Tokuyama, Japan) exchange membranes were carried out to remove KCl that existed in a large amount in the ion exchanged solution. The effects of operation voltage and feed composition on the performance of DSED were investigated. Based on the operating conditions determined above, IEC and DSED were applied in sequence to an ultrafiltered fermentation broth. Almost complete removal of KCl was possible with no significant loss of KCA, although the KCA recovery was slightly lower than that with the simulated fermentation broth. Based on this observation, it was concluded that IEC and DESD could be an effective process combination for the recovery of KCA from fermentation broth. © KSBB hÉóïçêÇëW=éçí~ëëáìã=Åä~îìä~å~íÉ=Eh`^FI=áçå=ÉñÅÜ~åÖÉ=ÅÜêçã~íçÖê~éÜó=Efb`FI=ÇÉë~äíáåÖ=ÉäÉÅíêçÇá~äóëáë=EapbaFI= = ÑÉêãÉåí~íáçå=ÄêçíÜ= = = = =

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Influence of temperature on the kinetics of adsorption and desorption of clavulanic acid by ionic exchange

Cited by 31 publications

References 6 publications

Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

Evaluation of kinetic and mass transfer parameters for adsorption of clavulanic acid into natural and synthetic zeolites

Recovery of potassium clavulanate from fermentation broth by ion exchange chromatography and desalting electrodialysis

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