Evaluation of the effects of the parameters involved in the purification of clavulanic acid from fermentation broth by aqueous two-phase systems

Abstract

The purification of clavulanic acid (CA), which is an important beta-lactam antibiotic produced by submerged cultivation of Streptomyces clavuligerus, was studied through the use of phosphate and polyethylene glycol-based aqueous two-phase systems. The parameters' effect on the yield and purification was evaluated through an experimental design and the preliminary results showed that the polyethylene molecular mass and tie-line length and phase volume ratio exerted the strongest effect on the yield and distribution coefficient in the range tested. In addition, the response surface methodology was used to optimize the distribution coefficient, yield, and purification factor. The optimal conditions of yield and purification factor are in the regions where polyethylene has a low molecular mass, pH close to the isoelectric point, and lower top phase volume. A 100% yield and a 1.5-fold purification factor are obtained when extracting CA by maximizing the conditions of an aqueous two-phase system.

Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum

“…According to Silva et al (2009) values of R 2 > 90% are very good in the experimental design of bioprocesses.…”

Extractive fermentation of clavulanic acid by Streptomyces DAUFPE 3060 using aqueous two‐phase system

“…As expected by these considerations, the highest partition coefficients of CA (19.4 and 13.5) were observed in runs 2 and 8 of the FFD, which were carried out at the highest PEG molar mass (8,000 g/mol) and using 15 and 25% of both phosphate salts and PEG, respectively. Under these conditions, the high PEG molar mass could have increased the hydrophobicity of the top phase,25 and the effect of exclusion volume could also have taken place, thus attracting CA more strongly. Albertsson3 reported also that the partition coefficients are higher than theoretically expected for small molecules such as CA.…”

Study on extraction and separation of butyric acid from clostridium tyrobutyricum fermentation broth in PEG/Na2SO4 aqueous two-phase system

“…The use of 9% Na 2 SO 4 resulted in a extraction yield of 87.8 8%, as Na 2 SO 4 a hydrophilic salt mainly, therefore mainly distributed in the sodium sulfaterich phase, an increase in Na 2 SO 4 concentration might reduce the water content in the PEG-rich phase and thus the phase's total volume. With a further increase of Na 2 SO 4, the volume and the water content of the sodium sulfate-rich phase increased, driving PEG and butyric acid bound to it, from the PEG-rich phase to the sodium sulfate-rich on, resulting in a drop in butyric acid yield [28].…”