Influence of Temperature on Symbiotic Bacterium Composition in Successive Generations of Egg Parasitoid, Anagrus nilaparvatae

Wang, Xin; Xu, Hongwei; Liu, Shuping; Tang, Jiangwu; Zheng, Xiaodong; Lv, Zhongxian

doi:10.1016/j.rsci.2016.06.003

Cited by 4 publications

(3 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results were similar to those of research on silkworm and egg parasitoids, as their bacterial communities also changed with high temperatures (Wang et al. , 2016; Sun et al. , 2017).…”

Section: Discussionsupporting

confidence: 89%

“…In this experiment on the LS population, the results indicated that high temperature (31°C) significantly decreased the bacterial community diversity in B. tabaci MED. These results were similar to those of research on silkworm and egg parasitoids, as their bacterial communities also changed with high temperatures (Wang et al, 2016;Sun et al, 2017).…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Infection density pattern of Cardinium affects the responses of bacterial communities in an invasive whitefly under heat conditions

et al. 2022

View full text Add to dashboard Cite

Communities of bacteria, especially symbionts, are vital for the growth and development of insects and other arthropods, including Bemisia tabaci Mediterranean (MED), a destructive and invasive insect pest. However, the infection density patterns and influence factors of bacteria in whiteflies, which mainly include symbionts, remain largely unclear. To reveal the different density patterns of Cardinium in B. tabaci MED populations and the impacts of high temperatures on whiteflies with different Cardinium density infection patterns, 2 isofemale lines isolated from B. tabaci MED from the same geographical population of China and from B. tabaci MED collected from other countries and locations were examined using several techniques and methods, including fluorescence in situ hybridization (FISH), quantitative real‐time polymerase chain reaction (qPCR), 16S rRNA gene sequencing, and 2b‐RAD sequencing. The results showed that there were 2 different infection density patterns of Cardinium in B. tabaci MED (including 1 high‐density pattern and 1 low‐density pattern). For whiteflies with low‐density Cardinium, conventional PCR could not detect Cardinium, but the other techniques confirmed that there was a low level of Cardinium within hosts. High temperature significantly decreased the diversity of bacterial communities: the relative titer of Cardinium increased but the density of Rickettsia decreased in the isofemale line with high‐density Cardinium. However, high temperature did not influence the diversity and symbiont density in the line with low‐density Cardinium. Moreover, high temperature influenced the functions of bacterial communities in whiteflies with high‐density Cardinium but did not affect the bacterial functions in whiteflies with low‐density Cardinium. Our results provide novel insights into the complex associations between symbionts and host insects.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 88%

Infection density pattern of Cardinium affects the responses of bacterial communities in an invasive whitefly under heat conditions

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The dominant bacterial species in different generations of insects are stable under low temperatures, but can significantly change under high temperatures. High temperature reduces the number of some symbiotic bacterial species in insects [47]. Possibly, the microbes allow surviving invertebrates at low temperatures and this is why the changes in the species composition of the host’s microbiome due to temperature changes were observed.…”

Section: Discussionmentioning

confidence: 99%

First Evidence of Intracellular Bacteria Cardinium in Thermophilic Mite Microzetorchestes emeryi (Acari: Oribatida): Molecular Screening of Bacterial Endosymbiont Species

Konecka

Olszanowski

2019

Curr Microbiol

View full text Add to dashboard Cite

We undertook the issue of the distribution of intracellular bacteria among Oribatida (Acari). Six genera of bacteria were detected by PCR and Sanger DNA sequencing: Wolbachia , Cardinium , Rickettsia , Spiroplasma , Arsenophonus , and Hamiltonella . Our research, for the first time, revealed the presence of Cardinium in Microzetorchestes emeryi in two subpopulations separated from each other by 300 m. The percentages of infected animals were the same in both subpopulations—ca. 20%. The identity of 16S rDNA sequences of Cardinium between these two subpopulations of M. emeryi was 97%. Phylogenetic analysis showed that the Cardinium in M. emeryi was clustered into the group A. The occurrence of M. emeryi in Poland has not been reported before and our report is the first one. Cardinium maybe help the thermophilic M. emeryi to adapt to low temperatures in the Central Europe.

show abstract

Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines

Horgan

Srinivasan

Crisol‐Martínez

et al. 2019

Ecology and Evolution

View full text Add to dashboard Cite

The microbiomes of phloem‐feeding insects include functional bacteria and yeasts essential for herbivore survival and development. Changes in microbiome composition are implicated in virulence adaptation by herbivores to host plant species or host populations (including crop varieties). We examined patterns in adaptation by the green leafhopper, Nephotettix virescens, to near‐isogenic rice lines (NILs) with one or two resistance genes and the recurrent parent T65, without resistance genes. Only the line with two resistance genes was effective in reducing leafhopper fitness. After 20 generations on the resistant line, selected leafhoppers attained similar survival, weight gain, and egg laying to leafhoppers that were continually reared on the susceptible recurrent parent, indicating that they had adapted to the resistant host. By sequencing the 16s rRNA gene, we described the microbiome of leafhoppers from colonies associated with five collection sites, and continually reared or switched between NILs. The microbiomes included 69–119 OTUs of which 44 occurred in ≥90% of samples. Of these, 14 OTUs were assigned to the obligate symbiont Candidatus sulcia clade. After 20 generations of selection, collection site had a greater effect than host plant on microbiome composition. Six bacteria genera, including C. sulcia, were associated with leafhopper virulence. However, there was significant within‐treatment, site‐related variability in the prevalence of these taxa such that the mechanisms underlying their association with virulence remain to be determined. Our results imply that these taxa are associated with leafhopper nutrition. Ours is the first study to describe microbiome diversity and composition in rice leafhoppers. We discuss our results in light of the multiple functions of herbivore microbiomes during virulence adaptation in insect herbivores.

show abstract

Influence of Temperature on Symbiotic Bacterium Composition in Successive Generations of Egg Parasitoid, Anagrus nilaparvatae

Cited by 4 publications

References 30 publications

Infection density pattern of Cardinium affects the responses of bacterial communities in an invasive whitefly under heat conditions

Infection density pattern of Cardinium affects the responses of bacterial communities in an invasive whitefly under heat conditions

First Evidence of Intracellular Bacteria Cardinium in Thermophilic Mite Microzetorchestes emeryi (Acari: Oribatida): Molecular Screening of Bacterial Endosymbiont Species

Microbiome responses during virulence adaptation by a phloem‐feeding insect to resistant near‐isogenic rice lines

Contact Info

Product

Resources

About