Influence of temperature and substrate conditions on the omt-1 gene expression of Aspergillus parasiticus in relation to its aflatoxin production

Lozano‐Ojalvo, Daniel; Rodríguez, Alicia; Bernáldez, Victoria; Córdoba, Juan J.; Rodrı́guez, Mar

doi:10.1016/j.ijfoodmicro.2013.07.011

Cited by 25 publications

(15 citation statements)

References 57 publications

(77 reference statements)

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“…It was found that the BHA treatment had no direct effect on the degradation of FB1 ( Figures 3C,D ), which suggested that the influence of BHA appeared to be only at the transcriptional period. The results were in agreement with the previous reports of Lazzaro et al (2012b) and Lozano-Ojalvo et al (2013) , who reported that the biosynthesis of mycotoxin was regulated at a genetic level. Therefore, our results suggested that reduced expression of the FUM1 and FUM8 genes might be involved in the decrease in fumonisin content by BHA treatment.…”

Section: Discussionsupporting

confidence: 93%

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Jian

Chen

et al. 2016

Front. Microbiol.

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Fusarium proliferatum as a common fungus pathogen in foods can produce toxic fumonisins, which can cause animal diseases and increase risks of human cancers. On contrary, butylated hydroxyanisole (BHA) as a synthetic antioxidant offers a clue for preventing growth of fungal species and inhibiting production of mycotoxins. Unfortunately, information of the inhibitory mechanism of BHA on Fusarium species is still limited. In this study, influence of BHA treatment on growth and inhibition of fumonisin production in relation to the expression of the fumonisin biosynthesis-related genes of the F. proliferatum ZYF was investigated, which revealed that BHA had a negative influence on growth and fumonisin production of F. proliferatum. To further elucidate the mechanism of BHA on the growth of F. proliferatum, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to examine the F. proliferatum hyphae. The BHA treatment induced the loss of cytoplasm and cellular constituents, as well as distortion of mycelia, but it did not directly degrade the fumonisin. Furthermore, the BHA treatment markedly inhibited the expressions of FUM1 (a polyketide synthase encoding gene) and FUM8 (an aminotransferase encoding gene) genes, which resulted in the depression of metabolic pathway of F. proliferatum. The transcriptional analyses of the FUM1 and FUM8 genes confirmed a correlation between the fumonisin production and its gene expression. This study provided some insights into mechanisms of production of fumonisin and feasible prevention to reduce fumonisin contamination in favor of human and animal health.

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Section: Discussionsupporting

confidence: 93%

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Jian

Chen

et al. 2016

Front. Microbiol.

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“…The effect of ROS on aflatoxin synthesis has been related to redox balance through β-oxidation, as studied through foxA gene expression [ 23 ]. Besides, the aflP gene has been used to study the effect of environmental factors on aflatoxin production [ 26 ]. Thus, aflP and foxA expression was assessed to study the effect of PgAFP and the other two biocontrol agents on aflatoxin production.…”

Section: Discussionmentioning

confidence: 99%

“…For this, the seven following treatments were tested in YES broth and Ca-YES broth: PgAFP alone (Pg), D. hansenii alone (Dh), P. acidilactici alone (Pa), PgAFP combined with D. hansenii (Pg + Dh), PgAFP combined with P. acidilactici (Pg + Pa), D. hansenii combined with P. acidilactici (Dh + Pa), and PgAFP combined with D. hansenii and P. acidilactici (Pg + Dh + Pa). Samples were incubated statically for 15 days at 25 °C to stimulate aflatoxin production [ 26 ]. All the assays were carried out in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…To stimulate aflatoxin production, all batches were incubated at 25 °C [ 26 ]. Samples were taken after 5 and 15 days of incubation.…”

Section: Methodsmentioning

confidence: 99%

“…Expression of gene foxA has been used as a marker for β-oxidation in A. flavus [ 24 ] and A. parasiticus [ 25 ]. Similarly, the expression of gene aflP , coding for O-methyltransferase A, has been used to monitor aflatoxin biosynthesis [ 26 , 27 ]. Therefore, studying the expression of these two genes could provide information on whether PgAFP treatment increases aflatoxin production by oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effect of PgAFP and Protective Cultures on Aspergillus parasiticus Growth and Aflatoxins Production on Dry-Fermented Sausage and Cheese

et al. 2018

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Aflatoxigenic molds can grow and produce aflatoxins on dry-fermented meat and cheese. The small, basic, cysteine-rich antifungal protein PgAFP displays a time-limited inhibitory ability against unwanted molds by increasing reactive oxygen species (ROS), which can lead to increased aflatoxin production. However, calcium abolishes the inhibitory effect of PgAFP on certain Aspergillus spp. To maximize the antifungal effect, this protein may be combined with protective cultures. Yeasts and lactic acid bacteria may counteract the impact of calcium on PgAFP fungal inhibition. The objective of this work was to study the effect of PgAFP and different combined treatments with Debaryomyces hansenii and/or Pediococcus acidilactici against growth of and aflatoxin production by an aflatoxigenic strain of Aspergillus parasiticus in both culture media and dry-fermented foods with low or high calcium levels. Aflatoxins production was increased by PgAFP but dramatically reduced by P. acidilactici in low calcium culture medium, whereas in the Ca-enriched culture medium, all treatments tested led to low aflatoxins levels. To study whether PgAFP and the protective microorganisms interfere with ROS and aflatoxin production, the relative expression of genes foxA, which is involved in peroxisomal β-oxidation, and aflP, which is required for aflatoxin biosynthesis, were evaluated. The aflatoxin overproduction induced by PgAFP seems not to be linked to peroxisomal β-oxidation. The combination of PgAFP and D. hansenii provided a successful inhibitory effect on A. parasiticus growth as well as on aflatoxin production on sliced dry-fermented sausage and cheese ripened up to 15 days, whereas P. acidilactici did not further enhance the protective effect of the two former agents. Therefore, the combined treatment of PgAFP and D. hansenii seems to provide a promising protective mean against aflatoxin-producing A. parasiticus on dry-fermented foods.

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Gene Expression Analysis as a Method to Predict OTA Accumulation in Dry-Cured Meat Products

et al. 2018

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Influence of temperature and substrate conditions on the omt-1 gene expression of Aspergillus parasiticus in relation to its aflatoxin production

Cited by 25 publications

References 57 publications

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Influence of Butylated Hydroxyanisole on the Growth, Hyphal Morphology, and the Biosynthesis of Fumonisins in Fusarium proliferatum

Inhibitory Effect of PgAFP and Protective Cultures on Aspergillus parasiticus Growth and Aflatoxins Production on Dry-Fermented Sausage and Cheese

Gene Expression Analysis as a Method to Predict OTA Accumulation in Dry-Cured Meat Products

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