Influence of temperature and photoperiod on cytokinin pools in the potato, Solanum tuberosum L.

Langille, Alan R.; Forsline, Philip L.

doi:10.1016/0304-4211(74)90115-1

Cited by 19 publications

(7 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results of the present study differed somewhat from others conducted in this laboratory (18 Further evidence that this rise in ZR levels signaled entrance into the state to tuberize comes from an earlier study (12) where we observed that percentage tuberization of nodal stem segments from induced plants was significantly higher than noninduced plants when cultured in vitro. With the addition of kinetin to the medium, however, this relationship was no longer apparent, ie.…”

Section: Discussioncontrasting

confidence: 99%

“…Similarly, Palmer and Smith (26) have demonstrated the requirement for cytokinins for in vitro tuberization of excised rhizomes. Results of this laboratory (11,12,18) have supported the theory that the tuberizing factor may be cytokininlike in nature, in that total cytokinin activity was significantly higher in induced than noninduced plants. Two of the four cytokinin compounds separated from potato tissues were significantly higher in induced plants, reaching their highest values in below-ground tissues after 6 'To whom reprint requests should be sent.…”

supporting

confidence: 60%

“…This compound has been found in other plant tissues, such as cotton ovules (27), pinto bean fruit (16), sycamore sap (15) and crown gall tumor tissue (22). Results of this laboratory (18) revealed that induced potato tissue was significantly higher in total cytokinin activity than noninduced tissue. Although two of the four cytokinins separated by paper chromatography were higher in induced tissue, no attempt was made to elucidate their structures (1 1).…”

Section: Discussionmentioning

confidence: 83%

See 2 more Smart Citations

Physiology of Tuberization in Solanum tuberosum L

Mauk

Langille²

1978

Plant Physiol.

100

View full text Add to dashboard Cite

Using high pressure liquid chromatography, the cucumber cotyledon bioassay, and mass spectrometry a cytokinin isolated from Solaum tuberoswn L. cv. Katahdin plant tissues has been identified as cis-zeatin riboside. Zeatin riboside (ZR) levels in plants grown under inducing conditions (28 C day and 13 C night with a 10-hour photoperiod) were significantly higher than those in plants grown under noninducing conditions (30 C day and 28 C night with an 18-hour photoperiod). The highest level of ZR was noted in below-ground tissue after 4 days exposure to inducing conditions, with tuber initiation observed after 8 days. A companion study conducted to determine the effect of ZR on in vitro tuberization of noninduced rhizomes revealed that after 1 month in culture, controls exhibited 0% tuberization, while ZR treatments of 0.3 and 3.0 milligrams per liter showed 39 and 75% tuberization, respectively.The tuberization process in the potato (Solanum tuberosum L.) is thought to be controlled by environmental factors, mainly photoperiod and temperature, which regulate levels ofendogenous growth substances. Short days and cool night temperatures (inducing conditions) have been reported to favor tuberization while long days and high night temperatures delay or inhibit the process (9,13,28).Several workers (7,8,21) have suggested the existence of a tuber-forming stimulus which is formed in leaves under inducing conditions and translocated to below-ground tuberization sites.Other workers have demonstrated the movement of a tuber-forming stimulus from an induced scion through a graft union to below-ground rhizomes which subsequently tuberized (17,25).Although the exact nature of this tuber-forming stimulus remains unknown, it has been speculated that it may be similar to cytokinins (6). Similarly, Palmer and Smith (26) have demonstrated the requirement for cytokinins for in vitro tuberization of excised rhizomes. Results of this laboratory (11,12,18) have supported the theory that the tuberizing factor may be cytokininlike in nature, in that total cytokinin activity was significantly higher in induced than noninduced plants. Two 'To whom reprint requests should be sent.In an attempt to elucidate the structure of potato cytokinins, Anstis and Northcote (1) reported isolating a compound which co-chromatographed with ZR.5 Recently, Van Staden (31) found an N-6-substituted adenyl compound in potato tissue using MS. Accordingly, the objectives of the present study were 2-fold: first, to attempt to isolate and identify the major cytokinin in Katahdin potato tissues; and second, to measure levels of this cytokinin in the plant in an effort to ascertain its possible role in the tuberization process.MATERIALS AND METHODS Plant Tissue. Plant material used in this study was obtained by planting tubers of S. tuberosum L. cv. Katahdin in flats of moist Perlite. Tubers were incubated in a growth chamber adjusted to 28 C day and 25 C night with a 16-hr photoperiod. Growth chambers provided a light intensity of 660 ft-c at pot level. ...

show abstract

Section: Discussioncontrasting

confidence: 99%

supporting

confidence: 60%

Section: Discussionmentioning

confidence: 83%

See 1 more Smart Citation

Physiology of Tuberization in Solanum tuberosum L

Mauk

Langille²

1978

Plant Physiol.

100

View full text Add to dashboard Cite

show abstract

“…Gas exchange measurements were done according to standard physiological procedures using a multichannel open gas exchange system as described by Lawlor et aL [24]. Measurements were on first fully expanded leaves and were averages of simultaneous recordings for three leaves.…”

Section: Physiological and Biochemical Assaysmentioning

confidence: 99%

Phenotypic changes in T-cyt-transformed potato plants are consistent with enhanced sensitivity of specific cell types to normal regulation by root-derived cytokinin

et al. 1991

View full text Add to dashboard Cite

From over forty independently isolated potato lines transformed with wild-type and promoter-mutated T-cyt genes, a number of lines were selected for examination of phenotypic changes in growth and development for plants grown in soil in a controlled environment. The three lines chosen for most detailed examination showed a wide spectrum of phenotypic changes. In comparisons with control potato cv. Désirée, the plants of one line had a two- to three-fold increase in biomass production during early vegetative growth, advanced senescence and a shortened plant life-span. Another line showed abnormal cellulytic senescence. In two lines there were increases in tuber numbers and more skewed tuber size distributions which correlated with reduced shoot apical dominance and shortened dormancy of the stored tubers. None of the lines showed altered timing of onset of tuberization or flowering, although tuberization was consistently delayed when expressed as a function of increasing total plant weight. A hypothesis is proposed to explain the diverse phenotypes which postulates that (1) T-cyt transformation causes enhanced sensitivity to cytokinins in specific types of shoot cells which are already targets for regulation by normal root-derived cytokinins; (2) two distinct types of shoot target cells are present, one in shoot meristems and one in leaves; (3) the two types can acquire enhanced sensitivity, either separately or in combination depending on the particular T-cyt transformation event. The scope for using the transformed plants in subsequent physiological, biochemical and molecular studies, aimed at examining the molecular basis of the model or selected consequences of T-cyt transformation in altering regulation of potato plant growth and development, is discussed. The attention is drawn to the possible involvement at the subcellular level of sucrose phosphate synthase in mediating the phenotypic effects caused by T-cyt transformation.

show abstract

“…There have been several reports showing that there are elevated levels of cytokinins in response to conditions which promote tuberization (Forsline and Langille 1975;Okazawa 1970;Langille and Forsline 1974;Mauk and Langille 1978;Arteca et al 1980). The major cytokinin found in potato leaves is zeatin riboside (Mauk and Langille 1978;Arteca et al 1980), although, zeatin has also been found in potato tubers (Okazawa 1970), leaves and roots (Arteca et a1.…”

Section: Cytokininsmentioning

confidence: 99%

Plant Growth Substances

Arteca¹

1996

166

View full text Add to dashboard Cite

Influence of temperature and photoperiod on cytokinin pools in the potato, Solanum tuberosum L.

Cited by 19 publications

References 6 publications

Physiology of Tuberization in Solanum tuberosum L

Physiology of Tuberization in Solanum tuberosum L

Phenotypic changes in T-cyt-transformed potato plants are consistent with enhanced sensitivity of specific cell types to normal regulation by root-derived cytokinin

Plant Growth Substances

Contact Info

Product

Resources

About