Influence of temperature and pH on the production of biosurfactant, bacteriocin and lactic acid by<i>Lactococcus lactis</i>CECT-4434

Souza, Elias Costa de; Azevedo, Pamela de Souza de Oliveira; Domínguez, José Manuel; Converti, Attílio; Oliveira, Ricardo Pinheiro de Souza

doi:10.1080/19476337.2017.1306806

Cited by 26 publications

(10 citation statements)

References 32 publications

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“…On the other hand, it has been demonstrated that some biosurfactants have antimicrobial activities in addition to their surfactant capacity (Das, Mukherjee, & Sen, 2008). This characteristic can be an additional advantage for the incorporation of these secondary metabolites in different sectors including pharmaceutical, cosmetic and food industry (López-Prieto, Rodríguez-López, Rincón-Fontán, Moldes, & Cruz, 2019;Ron & Rosenberg, 2001; Souza, Oliveira de Souza de Azevedo, Domínguez, Converti, & Pinheiro de Souza Oliveira, 2017;Vecino, Barbosa-Pereira, Devesa-Rey, Cruz, & Moldes, 2015a).…”

Section: Introductionmentioning

confidence: 99%

“…Although most of microbial biosurfactants are produced extracellularly, some microorganisms, including lactic acid bacteria like Lactobacillus pentosus, Lactobacillus paracasei or Lactococcus lactis, can produce cell-bound biosurfactants (Rodrigues, Moldes, Teixeira, & Oliveira, 2005;Souza et al, 2017;Vecino, Barbosa-Pereira, Devesa-Rey, Cruz, & Moldes, 2015c;Vecino et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a microorganism that produces biosurfactants in corn steep water

López-Prieto

Martínez-Padrón

Rodríguez‐López

et al. 2019

CyTA - Journal of Food

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In this work a Bacillus strain with capacity to produce biosurfactants was isolated from commercial corn steep water (CSW). The identification of this bacterium was based on phenotypic characteristics. This is a Gram-positive Bacillus, macroscopically catalase-negative, that possesses high mobility and forms terminal endospore. The colonies formed by the isolated Bacillus strain are characterized by a creaming, dull and glistening phenotype with a small point-like elevation in the center. Regarding its antimicrobial activity, the isolated Bacillus strain was effective against Candida albicans and Staphylococcus aureus observing, in the presence of pathogenic strains, a phenotype dissociation, switching the colonies from a whitish to yellowish pigmentation. The isolated microorganism, contrarily to other Bacillus strains, has the ability to produce extracellular and cell-bound biosurfactants. The analyses revealed that both biosurfactant extracts are similar to the lipopeptide biosurfactant produced by Bacillus subtilis. Aislamiento y caracterización del microorganismo responsable de la producción de biosurfactantes en licores de lavado de maíz RESUMEN ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a microorganism that produces biosurfactants in corn steep water

López-Prieto

Martínez-Padrón

Rodríguez‐López

et al. 2019

CyTA - Journal of Food

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“…It is also observed that at 30 and 37°C, L. lactis NZ9800 (non‐nisin producer) has a slower μ max than L. lactis NZ9700 (nisin producer), regardless of the growth type (surface/planktonic) (Table S2). Nisin is produced in the exponential phase, and is known to aid in colony formation . More specifically, nisin is involved in intercellular communication through quorum sensing mechanisms, and in this way controls the expression of a variety of functions (biofilm formation, exopolysaccharide production, production of antimicrobial compounds, motility, protein expression, etc.)…”

Section: Resultsmentioning

confidence: 99%

A multi‐scale analysis of the effect of complex viscoelastic models on Listeria dynamics and adaptation in co‐culture systems

et al. 2019

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Natural antimicrobials are of interest to replace traditional food decontamination methods: they are milder and maintain desirable sensory characteristics. However, efficacy can be affected by food structure/composition, thus structural effects in a co-culture pathogen/microflora system are investigated. Listeria was grown planktonically (liquid broth) or on a biphasic viscoelastic system, in monoculture with/without artificial nisin, or in co-culture with Lactococcus lactis (nisin/non-nisin producing). Microbial growth kinetics were monitored and advanced microscopy techniques were utilized to quantify cellular interactions and spatial organization. Microstructural effects are observed on the kinetics, with differences in monoculture/co-culture. Significant microscopic differences are observed in spatial organization and colony size. We are the first to observe changing growth location for all species in monoculture/co-culture, with differences in colony size/organization through stationary phase. This study provides insight into the environmental stress response/adaptation of Listeria grown on structured systems in response to L. lactis and natural antimicrobials.

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“…Moreover, the scale-up of biosurfactant production using GRAS will require simple microbiological practices and instruments. Some LABs such as Lactobacillus brevis , Lactobacillus paracasei, Lactobacillus plantarum , and Lactococcus lactis produce extracellular and cell-bound biosurfactants with different properties simultaneously ( Cornea et al, 2016 ; Souza et al, 2017 ; Vecino et al, 2017 ). Thus, the production of two biosurfactants by using an LAB strain will be relatively cost-effective and convenient.…”

Section: Introductionmentioning

confidence: 99%

Reuse of Immobilized Weissella cibaria PN3 for Long-Term Production of Both Extracellular and Cell-Bound Glycolipid Biosurfactants

Subsanguan

Khondee

Nawavimarn

et al. 2020

Front. Bioeng. Biotechnol.

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Lactic acid bacteria (LABs) are generally recognized as safe (GRAS), and therefore, LAB biosurfactants are beneficial with negligible negative impacts. This study aims to maintain the biosurfactant producing activity of an LAB strain, Weissella cibaria PN3, by immobilizing the bacterial cells on a commercial porous carrier. For biosurfactant production, 2% soybean oil was used as the carbon source. After 72 h, immobilized cells were reused by replacing production medium. The extracellular and cell-bound biosurfactants were extracted from the resulting cell-free broth and cell pellets, respectively. SEM images of used immobilizing carriers showed increased surface roughness and clogged pores over time. Thus, the immobilizing carriers were washed in PBS buffer (pH 8.0) before reuse. To maintain biosurfactant production activity, immobilized cells were reactivated every three production cycles by incubating the washed immobilizing carriers in LB medium for 48 h. The maximum yields of purified extracellular (1.46 g/L) and cell-bound biosurfactants (1.99 g/L) were achieved in the 4th production cycle. The repeated biosurfactant production of nine cycles were completed within 1 month, while only 2 g of immobilized cells/L were applied. The extracellular and cell-bound biosurfactants had comparable surface tensions (31 – 33 mN/m); however, their CMC values were different (1.6 and 3.2 g/L, respectively). Both biosurfactants had moderate oil displacement efficiency with crude oil samples but formed emulsions well with gasoline, diesel, and lavender, lemongrass and coconut oils. The results suggested that the biosurfactants were relatively hydrophilic. In addition, the mixing of both biosurfactants showed a synergistic effect, as seen from the increased emulsifying activity with palm, soybean and crude oils. The biosurfactants at 10 – 16 mg/mL showed antimicrobial activity toward some bacteria and yeast but not filamentous fungi. The molecular structures of these biosurfactants were characterized by FTIR as different glycolipid congeners. The biosurfactant production process by immobilized Weissella cibaria PN3 cells was relatively cheap given that two types of biosurfactants were simultaneously produced and no new inoculum was required. The acquired glycolipid biosurfactants have high potential to be used separately or as mixed biosurfactants in various products, such as cleaning agents, food-grade emulsifiers and cosmetic products.

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Influence of temperature and pH on the production of biosurfactant, bacteriocin and lactic acid byLactococcus lactisCECT-4434

Cited by 26 publications

References 32 publications

Isolation and characterization of a microorganism that produces biosurfactants in corn steep water

Isolation and characterization of a microorganism that produces biosurfactants in corn steep water

A multi‐scale analysis of the effect of complex viscoelastic models on Listeria dynamics and adaptation in co‐culture systems

Reuse of Immobilized Weissella cibaria PN3 for Long-Term Production of Both Extracellular and Cell-Bound Glycolipid Biosurfactants

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