Influence of Tangeretin on Tamoxifen's Therapeutic Benefit in Mammary Cancer

Bracke, Marc; Depypere, Herman; Boterberg, Tom; Marck, Veerle L. Van; Vennekens, Kristel M.; Vanluchene, E.; Nuytinck, Margareta; Serreyn, Rudolphe; Mareel, Marc

doi:10.1093/jnci/91.4.354

Cited by 86 publications

(44 citation statements)

References 16 publications

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“…The inverted microscope was connected with an MTI CCD72 camera (Dage MTI, Michigan City, IN, USA) and a U-matic VO-5850P videorecorder (Sony, Tokyo, Japan) at 30 s intervals. Track lengths were measured as described (Bracke et al, 1997). Haptotaxis assays (Aznavoorian et al, 1990) were performed in 48-well microchemotaxis chambers (NeuroProbe Inc., Bethesda, MD, USA) with 8-mm pore size Nucleopore filters (Costar, Cambridge, MA, USA).…”

Section: Assays For Motilitymentioning

confidence: 99%

Bile acids stimulate invasion and haptotaxis in human colorectal cancer cells through activation of multiple oncogenic signaling pathways

et al. 2002

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Section: Assays For Motilitymentioning

confidence: 99%

Bile acids stimulate invasion and haptotaxis in human colorectal cancer cells through activation of multiple oncogenic signaling pathways

et al. 2002

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“…It should, however, be recognized that the assay fails to encompass all the elements of the microecosystem present in natural tumors, environments where for example immunological factors can influence the invasive behavior of the cancer cells. In at least one study, the absence of such factors in the assay has led to conflicting results between the outcomes of the chick heart assay 29 and those of an animal model 30 .…”

Section: Discussionmentioning

confidence: 99%

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Bracke¹,

Roman

Stevens

et al. 2015

JoVE

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The goal of the chick heart assay is to offer a relevant organ culture method to study tumor invasion in three dimensions. The assay can distinguish between invasive and non-invasive cells, and enables study of the effects of test compounds on tumor invasion. Cancer cells -either as aggregates or single cells -are confronted with fragments of embryonic chick heart. After organ culture in suspension for a few days or weeks the confronting cultures are fixed and embedded in paraffin for histological analysis. The three-dimensional interaction between the cancer cells and the normal tissue is then reconstructed from serial sections stained with hematoxylin-eosin or after immunohistochemical staining for epitopes in the heart tissue or the confronting cancer cells. The assay is consistent with the recent concept that cancer invasion is the result of molecular interactions between the cancer cells and their neighbouring stromal host elements (myofibroblasts, endothelial cells, extracellular matrix components, etc.). Here, this stromal environment is offered to the cancer cells as a living tissue fragment. Supporting aspects to the relevance of the assay are multiple. Invasion in the assay is in accordance with the criteria of cancer invasion: progressive occupation and replacement in time and space of the host tissue, and invasiveness and non-invasiveness in vivo of the confronting cells generally correlates with the outcome of the assay. Furthermore, the invasion pattern of cells in vivo, as defined by pathologists, is reflected in the histological images in the assay. Quantitative structure-activity relation (QSAR) analysis of the results obtained with numerous potentially anti-invasive organic congener compounds allowed the study of structure-activity relations for flavonoids and chalcones, and known anti-metastatic drugs used in the clinic (e.g., microtubule inhibitors) inhibit invasion in the assay as well. However, the assay does not take into account immunological contributions to cancer invasion. Video LinkThe video component of this article can be found at

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“…The blocking antibody specific for human E-cadherin, mouse mAb SHE78-7 (Takara Shuzo Co., Kyoto, Japan), was used at 2 mg/ml to disrupt E-cadherin mediated cell-cell adhesion. Bracke et al (1997) described the possibility that MTT assay might be modified without alteration of growth. We examined cell growth, defined as the final number of DLD-1 cells remaining after 1-3 days in culture, both by directly counting the number of cells after trypsin detachment and by MTT assay.…”

Section: Methodsmentioning

confidence: 99%

Expression of α-catenin in α-catenin-deficient cells results in a reduced proliferation in three-dimensional multicellular spheroids but not in two-dimensional monolayer cultures

Matsubara

Ozawa

2004

Oncogene

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a-Catenin is an intracellular protein that associates with the carboxy-terminal region of cadherin, a cell adhesion molecule, via b-catenin or c-catenin (plakoglobin). Linkage of cadherin to the cytoskeleton by catenins is required for full cadherin activity. Following transfection of an a-catenin-deficient colon carcinoma cell line with a series of a-catenin constructs, we discovered that the restoration of a-catenin expression results in reduced proliferation in three-dimensional multicellular spheroids, but not in twodimensional monolayer cultures. The cellular function of a-catenin has not been compared between cells in threeand two-dimensional culture; this is the first evidence that growth regulation in three-dimensional cultures requires signaling mediated by a-catenin. Two classes of constructs, containing deletions in either the central segment or the COOH terminus of the molecule, both induced morphological changes, including cell compaction, and suppressed cell growth in three-dimensional cultures. In a-catenin-expressing cells, inhibition of cadherin cell adhesion by treatment with anti-E-cadherin antibodies resulted in a similar phenotype as that observed following the loss of a-catenin. Therefore, both the homophilic interaction of the cadherin extracellular domain and the linkage of the cadherin cytoplasmic domain to the actin cytoskeleton by a-catenin are necessary for growth control in three-dimensional culture.

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Influence of Tangeretin on Tamoxifen's Therapeutic Benefit in Mammary Cancer

Cited by 86 publications

References 16 publications

Bile acids stimulate invasion and haptotaxis in human colorectal cancer cells through activation of multiple oncogenic signaling pathways

Bile acids stimulate invasion and haptotaxis in human colorectal cancer cells through activation of multiple oncogenic signaling pathways

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Expression of α-catenin in α-catenin-deficient cells results in a reduced proliferation in three-dimensional multicellular spheroids but not in two-dimensional monolayer cultures

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