Bile acids stimulate invasion and haptotaxis in human colorectal cancer cells through activation of multiple oncogenic signaling pathways

Debruyne, Philip R.; Bruyneel, Erik; Karaguni, Ioanna‐Maria; Li, X.; Flatau, Gilles; Müller, Oliver; Zimber, A.; Gespach, Christian; Mareel, Marcus

doi:10.1038/sj.onc.1205729

Cited by 72 publications

(55 citation statements)

References 60 publications

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“…Systemic host factors also might influence invasion. Candidates are steroid hormones [12], leptin [13], bile acids [14], and trefoil factors [15]. Taken together these observations underscore the influence of the host environment on the behaviour of malignant tumours in line with Paget's seed and soil hypothesis [16] as well as with more recent molecular data [17].…”

Section: Introductionsupporting

confidence: 58%

Role of tissue stroma in cancer cell invasion

Wever

Mareel

2003

The Journal of Pathology

924

813

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Section: Introductionsupporting

confidence: 58%

Role of tissue stroma in cancer cell invasion

Wever

Mareel

2003

The Journal of Pathology

924

813

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“…To test the hypothesis that invasion-stimulating soluble factors were present in desmoid CM, collagen invasion assays with HCT-8/ E11 colon cancer cells were performed. These colon cancer cells were used as a model because it was previously shown that they were noninvasive in type I collagen, but can become invasive depending on stimulation (Debruyne et al, 2002;Regnauld et al, 2002;Nguyen et al, 2002). In the presence of normal medium (DMEM), no invasion of the cells into the collagen was observed.…”

Section: Desmoid CM Is Able To Stimulate Invasionmentioning

confidence: 99%

Invasion and MMP expression profile in desmoid tumours

et al. 2004

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Desmoid tumours are locally invasive soft tissue tumours in which b-catenin mediated TCF-dependent transcription is activated. The role of soluble factors secreted by the myofibroblastic desmoid tumour, which could stimulate tumour invasiveness, was investigated. Using collagen gel invasion assays, the presence of factors stimulating invasion in desmoid conditioned media (CM) could be established. Since matrix metalloproteinases (MMPs) have been implicated in the process of tumoral invasion, the expression levels of the MMP family members were evaluated. Quantitative reverse transcription -PCR was used to determine the expression levels of MMP1, MMP2, MMP3, MMP7, MMP11, MMP12, MMP13, MMP14 and the inhibitors TIMP1, TIMP2 and TIMP3. Besides overexpression of MMP7, a known TCF-dependent target gene, a striking upregulation of the expression levels of MMP1, MMP3, MMP11, MMP12 and MMP13 in desmoid tumours, compared to unaffected fibroblasts from the same patients, was found. Treating the CM of desmoids with a synthetic and a physiologic MMP inhibitor reduced the invasion-stimulating capacity of the desmoid CM by approximately 50%. These results suggest the involvement of soluble factors, released by the desmoid cells, in stimulating invasion and implicate the MMPs as facilitators of invasion.

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“…The other molecules studied include MST1 (Mammalian sterile 20-like kinase) [6] . Replication protein (RPA) [7] , ELAV-like protein Huk and COX-2 [8] , a-catenin, b-catenin [9] a-ligatin, b-ligatin, Rho-a [10] , etc. In fact, an established cascade of events leading to colorectal cancer development and progression is described by Vogelstein.…”

Section: Introductionmentioning

confidence: 99%

TSPAN1 protein expression: A significant prognostic indicator for patients with colorectal adenocarcinoma

Chen

Zhu²,

Zhang³

et al. 2009

WJG

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AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma. METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 mRNA assay by RT-PCR. Eighty-eight specimens of human colorectal adenocarcinoma were surgically removed. TSPAN1 protein levels in cancer tissues were determined by immunohistochemistry using a polyclonal antibody against self-prepared TSPAN1. The correlation between TSPAN1 expression and the clinicopathological factors and the overall survival rate was analyzed by univariate and multivariate assay. RESULTS:TSPAN1 mRNA was detected in 90.0% (18/20) of cancerous tissues. The light density of TSPAN1 mRNA expression levels was 0.89 ± 0.30 in adenocarcinoma by gel-image system. TSPAN1 protein expression was detected in 78.41% (69/88) and weakly expressed in 40% normal colorectal tissues. There were significant differences between colorectal adenocarcinoma and normal control epithelium (P < 0.05). TSPAN1 protein expression in colorectal cancerous tissue was significantly correlated with the histological grade, cell expression PCNA, lymph nodal metastasis and TNM staging of the disease. Patients with TSPAN1 protein overexpression had a significantly shorter survival period than that in patients with TSPAN1 protein negative or weak expression, respectively (P < 0.05). Furthermore, by multivariate analysis, TSPAN1 protein expression demonstrated an independent prognostic factor for human colorectal cancers (P < 0.05, relative risk 0.755; 95% confidence interval 0.302-1.208). CONCLUSION:The expression of TSPAN1 gene is increased in colorectal carcinoma, suggesting that TSPAN1 might serve as an independent prognostic factor for the colorectal adenocarcinoma patients.

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Bile acids stimulate invasion and haptotaxis in human colorectal cancer cells through activation of multiple oncogenic signaling pathways

Cited by 72 publications

References 60 publications

Role of tissue stroma in cancer cell invasion

Role of tissue stroma in cancer cell invasion

Invasion and MMP expression profile in desmoid tumours

TSPAN1 protein expression: A significant prognostic indicator for patients with colorectal adenocarcinoma

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