Influence of surface properties of resin‐based composites on in vitro Streptococcus mutans biofilm development

Abstract

The aim of this in vitro study was to evaluate the influence of physicochemical surface properties of resin-based composites on Streptococcus mutans biofilm formation. Specimens were prepared from each of four resin-based composites by polymerization against Mylar strips. Half of the number of specimens received no further surface treatment, whereas the other half were subjected to a polishing treatment. Surface roughness (SR) and topography were assessed using profilometry and atomic force microscopy. Surface free-energy (SFE) was determined, and the chemical surface composition was analysed by X-ray photoelectron spectroscopy (XPS). S. mutans biofilms were formed on the surface of the resin-based composite specimens for either 48 or 96 h using an artificial mouth system (AMS). Polishing caused a significant decrease in SFE, and XPS analysis indicated an increase of surface silicon and a decrease of surface carbon. Only for Grandio was a significant increase in SR identified after polishing, which was probably related to the higher concentration of filler particles on its surface. Significantly less S. mutans biofilm formation was observed on polished resin-based composites than on unpolished resin-based composites. These results indicate that the proportions of resin matrix and filler particles on the surface of resin-based composites strongly influence S. mutans biofilm formation in vitro, suggesting that minimization of resin matrix exposure might be useful to reduce biofilm formation on the surface of resin-based composites.

Relevance of surface characteristics in the adhesiveness of polymicrobial biofilms to crown restoration materials

“…In the present study, because the SFE of all samples was approximately 50 mN/m, we presume that SFE can be altered by bacterial adhesion at 20 mN/m. Ionescu et al reported that SFE is affected more by initial bacterial adhesion than by long-term cultivated biofilms, which is inconsistent with our results (36). This difference may be attributable to the 24 h cultivation time in our study.…”

Effect of different initial finishes and Parylene coating thickness on the surface properties of coated PMMA

“…25,34,43 A limitation of most of the methods used for the SFE calculation though is that the Young equation assumes that the solid surface is rigid, chemically homogemous, and smooth on an atomic scale. 39 The selection of the probe liquids was also based on the experimental protocols of relevant studies.…”

Adhesion of Streptococcus Mutans Biofilm on the Surface of Indirect Resin Composites Restorations.

“…28 , while in ceram x one the lower bacterial adhesion may be due to the nano-ceramic filler used in its construction giving it a higher filler loading and smoother surface which yields a smoother surface with subsequent lower adhesion of bacteria, and this in agreement with the study conducted by Ionescu A who concluded that the proportions of resin matrix and filler particles on the surface of resinbased composites strongly influence S mutans biofilm formation in vitro, suggesting that minimization of resin matrix exposure might be useful to reduce biofilm formation on the surface of resinbased composites. 29 Both Z250 and Herculite classic have nearly the same tendency for bacterial adhesion also Z 250 used as indirect resin composite it was cured by the direct LED without any furnace which may yielded degree of conversion similar to that of Herculite, also they have similar filler size so under the same finishing and polishing procedure they gave nearly equal surface characters which explains the similarity in bacterial adhesion and this in accordance with Celik C et al who studied the effect of finishing and polishing procedures on surface roughness of tooth-colored materials and found that the smoothest surface was generated with Sof-Lex Pop-on disks for all the materials tested.…”