Influence of sulfur fumigation on glycoside profile in Platycodonis Radix (Jiegeng)

Ma, Xiaoqing; Li, Sumei; Chan, Chitat; Su, Tao; Li, Weidong; Cao, Hui; Wang, Fong; Yu, Zhi‐Ling

doi:10.1186/s13020-016-0101-1

Cited by 14 publications

(5 citation statements)

References 25 publications

(26 reference statements)

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“…Triterpene saponins, the most abundant component in PR, were also identified as major components [ 16 ]. The main cleavage pathway of triterpene saponins involves sequential intramolecular deglycosylation and interchain cleavage to produce characteristic fragment ions.…”

Section: Resultsmentioning

confidence: 99%

Platycodonis Radix Alleviates LPS-Induced Lung Inflammation through Modulation of TRPA1 Channels

et al. 2023

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Platycodonis Radix (PR), a widely consumed herbal food, and its bioactive constituents, platycodins, have therapeutic potential for lung inflammation. Transient Receptor Potential Ankyrin 1 (TRPA1), which is essential for the control of inflammation, may be involved in the development of inflammation in the lungs. The aim of this study was to determine the TRPA1-targeted effects of PR against pulmonary inflammation and to investigate the affinity of PR constituents for TRPA1 and their potential mechanisms of action. Using a C57BL/6J mouse lipopolysaccharides (LPS) intratracheal instillation pneumonia model and advanced analytical techniques (UPLC-Q-TOF-MS/MS, molecular docking, immuno-fluorescence), five platycodins were isolated from PR, and the interaction between these platycodins and hTRPA1 was verified. Additionally, we analyzed the impact of platycodins on LPS-induced TRPA1 expression and calcium influx in BEAS-2B cells. The results indicated that PR treatment significantly reduced the severity of LPS-triggered inflammation in the mouse model. Interestingly, there was a mild increase in the expression of TRPA1 caused by PR in healthy mice. Among five isolated platycodins identified in the PR extract, Platycodin D3 (PD3) showed the highest affinity for hTRPA1. The interaction between platycodins and TRPA1 was verified through molecular docking methods, highlighting the significance of the S5–S6 pore-forming loop in TRPA1 and the unique structural attributes of platycodins. Furthermore, PD3 significantly reduced LPS-induced TRPA1 expression and calcium ion influx in BEAS-2B cells, substantiating its own role as an effective TRPA1 modulator. In conclusion, PR and platycodins, especially PD3, show promise as potential lung inflammation therapeutics. Further research should explore the precise mechanisms by which platycodins modulate TRPA1 and their broader therapeutic potential.

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Section: Resultsmentioning

confidence: 99%

Platycodonis Radix Alleviates LPS-Induced Lung Inflammation through Modulation of TRPA1 Channels

et al. 2023

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“…Compound 30 belongs to flavonoids, namely cosmosiin, generates [M‐H] − ion at m/z 431, the remarkably higher abundance ion at m/z 268 is consistent with loss of the glucosyl, the ion m/z 240 was generated by losing of CO from the ion m/z 268. Compounds 10 was identified as Tangshenoside ∣ by comparing the fragment ions with the literature [23]. Compounds 5 and 10 have similar fragmentation behavior and molecular weight difference of 162 Da, presumably the molecular formula of compound 5 is C 23 H 32 O 13 , by searching the Science Finder database to match with structure.…”

Section: Resultsmentioning

confidence: 99%

A strategy to comprehensively and quickly identify the chemical constituents in Platycodi Radix by ultra‐performance liquid chromatography coupled with traveling wave ion mobility quadrupole time‐of‐flight mass spectrometry

Gao

Shu

et al. 2021

J of Separation Science

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Quick identification of the complex composition of traditional Chinese medicine only through liquid‐mass spectrometry technology is difficult. Especially the identification of isomers and co‐eluting compounds is even more difficult. In this study, an approach of multidimensional data modes based on ultra‐performance liquid chromatography coupled with traveling wave ion mobility quadrupole time‐of‐flight mass spectrometry was proposed to quickly and comprehensively identify the compounds in Platycodi Radix. First, data‐independent acquisition, high‐definition acquisition, and tandem mass spectrometry acquisition modes were used to acquire integrated multidimensional mass spectral data. Second, summarize the diagnostic ions of compounds according to the fragmentation pathway of references. Third, unknown compounds and isomers were identified via the UNIFI™ software with an in‐house library. Finally, a total of 87 compounds were identified, seven compounds were explicitly identified by comparing the retention time and fragment ions with the references. Fourteen compounds were first detected in the Platycodi Radix, four of them tentatively were identified by comparing with previous literature, eight compounds were observed and reported for the first time by comparing typical fragmentation pathway with the known standard substances in this paper. This research strategy has a certain potential for the analysis of complex components of other traditional Chinese medicine.

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“…As a canonical and well-known Chinese medicine formula, SLBZ-AP is first described in " e Prescriptions of the Bureau of Taiping People's Welfare Pharmacy" in Song-dynasty [19] and contains twenty-eight species of TCM as Dangshen, Fuling, Baizhu, Gancao, and so on . ere are chemical components as saccharides (Dang Shen, Fu Ling, Bai Bian Dou) [37,38], glycosides (Shan Yao, Jie Geng, Shan Zhu Yu, Bai Shao) [39][40][41][42], and flavonoids (Bai Zhu, Gan Cao) [43,44] mainly in SLBZ-AP. Saccharides have immunomodulation effects [37].…”

Section: Discussionmentioning

confidence: 99%

Antinociceptive Effects of Shenling Baizhu through PI3K‐Akt‐mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small‐Cell Lung Cancer

Feng

Han

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Shenling Baizhu additive powder (SLBZ-AP), a formulation of a variety of natural medicinal plants, has clinical efficacy in treating cancers in previous studies. We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investigated in the present study. Mice model of BMLC was made and treated with SLBZ-AP. Pain behavioral tests were performed to explore the effect on BMLC-induced pain in mice. TUNEL staining was used to investigate apoptosis. The mRNA expression of markers in the PI3K/Akt/mTOR pathway was measured by qPCR, and protein expression was detected by western blotting and immunohistochemistry analysis. SLBZ-AP relieved BMLC-induced pain and prolonged animals’ survival, promoted cell apoptosis in the marrow from the tibia of BMLC mice, and inhibited mRNA and protein expression of AKT, mTOR, P70S6, and VEGF, as well as protein expression of p-AKT, p-mTOR, p-P70S6, and VEGF upregulation in the marrow of tibia induced by BMLC, an effect which was similar to rapamycin. Our results suggested that SLBZ-AP may have antinociceptive effect and prolong survival of BMLC mice at least partially by inhibiting cell proliferation and promoting apoptosis through the PI3K/Akt/mTOR signaling pathway. SLBZ-AP may be a potential candidate for BMLC therapy.

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Influence of sulfur fumigation on glycoside profile in Platycodonis Radix (Jiegeng)

Cited by 14 publications

References 25 publications

Platycodonis Radix Alleviates LPS-Induced Lung Inflammation through Modulation of TRPA1 Channels

Platycodonis Radix Alleviates LPS-Induced Lung Inflammation through Modulation of TRPA1 Channels

A strategy to comprehensively and quickly identify the chemical constituents in Platycodi Radix by ultra‐performance liquid chromatography coupled with traveling wave ion mobility quadrupole time‐of‐flight mass spectrometry

Antinociceptive Effects of Shenling Baizhu through PI3K‐Akt‐mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small‐Cell Lung Cancer

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