Antinociceptive Effects of Shenling Baizhu through PI3K‐Akt‐mTOR Signaling Pathway in a Mouse Model of Bone Metastasis with Small‐Cell Lung Cancer

Abstract

Shenling Baizhu additive powder (SLBZ-AP), a formulation of a variety of natural medicinal plants, has clinical efficacy in treating cancers in previous studies. We explored the effect of SLBZ-AP in bone metastasis of lung cancer (BMLC) mice, and the possible mechanism involved was further investigated in the present study. Mice model of BMLC was made and treated with SLBZ-AP. Pain behavioral tests were performed to explore the effect on BMLC-induced pain in mice. TUNEL staining was used to investigate apoptosis. The mRNA expression of markers in the PI3K/Akt/mTOR pathway was measured by qPCR, and protein expression was detected by western blotting and immunohistochemistry analysis. SLBZ-AP relieved BMLC-induced pain and prolonged animals’ survival, promoted cell apoptosis in the marrow from the tibia of BMLC mice, and inhibited mRNA and protein expression of AKT, mTOR, P70S6, and VEGF, as well as protein expression of p-AKT, p-mTOR, p-P70S6, and VEGF upregulation in the marrow of tibia induced by BMLC, an effect which was similar to rapamycin. Our results suggested that SLBZ-AP may have antinociceptive effect and prolong survival of BMLC mice at least partially by inhibiting cell proliferation and promoting apoptosis through the PI3K/Akt/mTOR signaling pathway. SLBZ-AP may be a potential candidate for BMLC therapy.

Prevention of polycystic ovary syndrome and postmenopausal osteoporosis by inhibiting apoptosis with Shenling Baizhu powder compound

“…Traditionally, SBP is used to treat inflammation, antioxidants, immunomodulators, and to promote apoptosis ( Tang et al, 2020 ; Wang et al, 2021 ; Pan et al, 2021 ). Inflammatory diseases, endocrine diseases, tumors, and other diseases have been demonstrated to benefit from it ( Tang et al, 2020 ; Feng et al, 2020 ). Specifically, this study investigated the potency of SBP on AHR.…”

“…The traditional Chinese herbal compound Shenling Baizhu powder (SBP) is used to treat inflammation, antioxidants, immunomodulators, and to promote apoptosis ( Tang et al, 2020 ; Wang et al, 2021 ; Pan et al, 2021 ). It has shown significant value in inflammatory diseases, endocrine diseases, tumors and other diseases ( Tang et al, 2020 ; Feng et al, 2020 ). However, the effect of the SBP compound on PCOS and PMO is unclear.…”

Efficacy and safety of Shen-Ling-Bai-Zhu-San combined with chemotherapy for lung cancer

“…Studies have shown that it can activate the PI3K-Akt-mTOR signaling pathway, adjust the microenvironment of the intestinal flora, and improve immunity. [ 15 , 17 , 18 ] Te-Hsin Chao et al found that SLBZS can stimulate the secretion of ghrelin to increase food intake and has potential anti-tumor effects. [ 24 ]…”

“…[ 14 ] The SLBZS has clinical effects on the treatment of LC, which possible mechanisms of action include activation of PI3K-Akt-mTOR Signaling Pathway and inhibition of tumor growth promoters and antiapoptotic proteins enhances proapoptotic proteins. [ 15 , 16 ] Moreover, SLBZS can adjust the microenvironment of the intestinal flora, improve the body's immunity, and ultimately increase the efficacy of chemotherapy and reduce the side effects. [ 17 , 18 ]…”

Small Cell Lung Cancer: State of the Art of the Molecular and Genetic Landscape and Novel Perspective

“…Feng et al recently published the effect of a Chinese medicinal formula, Baizhu Additive Powder (SLBZ-AP), on the pain control and survival of mice with metastatic lung cancer to the bone. It is postulated that SLBZ-AP partially exerts its effects through the PI3K/AKT/mTOR pathway [ 22 ].…”