Influence of substrate concentration on the induction of amidases in herbicide degradation

Lechner, Ute; Straube, G.

doi:10.1002/jobm.3630240817

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…Likewise, it has previously been proposed that most enzymes involved in the degradation of xenobiotic compounds are inducible (Guerin & Boyd, 1995). In accordance with the suggestions on critical concentrations for induction of degradative activity (Lechner & Straube, 1984;Schmidt et al, 1987), this could then be the weak point in using herbicide-mineralizing strains for remediation of water contaminated with low herbicide concentrations. Using induced cells pregrown with the contaminant of interest might solve this problem, but it remains unclear whether augmented cells will continue their degradative activity when the contaminant concentrations are below the level sustaining growth.…”

Section: Discussionmentioning

confidence: 59%

“…A basal level of enzyme activity at low substrate concentrations vs. an induced activity at higher concentrations have been described previously for halophenol (He & Sanford, 2002), trichlorobenzene (Rapp, 2001), naphthalene (Guerin & Boyd, 1995) and methyl tertbutyl ether (Schmidt et al, 2008)-degrading bacterial strains. In contrast, however, a cease in the degradation activity by different bacteria on reducing the contaminant concentration has been described in several other studies (Lechner & Straube, 1984;Schmidt et al, 1987;van der Meer et al, 1987).…”

Section: Discussionmentioning

confidence: 89%

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Constitutive mineralization of low concentrations of the herbicide linuron by aVariovoraxsp. strain

Sørensen

Simonsen

Aamand

2009

FEMS Microbiology Letters

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The mineralization of the herbicide linuron at concentrations of microg and mg L(-1) was studied in liquid batch experiments with Variovorax sp. strain SRS16. The strain was highly efficient at mineralizing a range of linuron concentrations (0.002-10 mg L(-1)) with 20-60% of the added (14)C-ring-labeled linuron metabolized to (14)CO(2) within hours to days depending on the initial linuron concentration and incubation period. At mg L(-1) linuron concentrations the mineralization activity by SRS16 was inducible and a shift to constitutive mineralization activity was apparent with a reduction in the linuron concentration to microg L(-1) levels. This study revealed that strain SRS16 is a promising candidate for bioaugmentation of water or soil resources contaminated with low linuron concentrations.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 89%

Constitutive mineralization of low concentrations of the herbicide linuron by aVariovoraxsp. strain

Sørensen

Simonsen

Aamand

2009

FEMS Microbiology Letters

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“…A Rhodococcus sp. that could use the anilide N-phenyl-propionamide as the sole source of carbon and energy has been described (Lechner and Straube 1984). The organism possessed an aryl acylamidase activity which was present constitutively at low levels when the organism was grown in a minimal medium and which was induced to higher levels by various anilides.…”

Section: Introductionmentioning

confidence: 99%

Aryl acylamidase from Rhodococcus erythropolis NCIB 12273

Vaughan¹,

Hall

Best

1990

Appl Microbiol Biotechnol

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A Rhodococcus erythropolis strain was isolated from soil on the basis of its ability to use acetaminophen as the sole source of both carbon and energy for growth. When grown in a complex medium containing an anilide inducer compound, the bacterium exhibited aryl acylamidase (EC 3.5.1.13) activity. This activity was not subject to carbon or nitrogen repression by the growth medium constituents as the enzyme was present throughout the exponential growth phase. The anilide was converted to the corresponding aniline, which was not further degraded. The enzyme was partially purified by a variety of methods including a batch ion exchange procedure, column ion exchange chromatography and hydrophobic interaction chromatography. The enzyme had a maximum activity at around pH 8.0 and had a Km for acetaminophen of 0.11 mM. Electrochemical assays of aryl acylamidase activity are described. The enzyme is suitable for use as a reagent in the clinical diagnostic measurement of acetaminophen.

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Use of salicylate to estimate the “threshold” inducer level for de novo synthesis of the phenol‐degrading enzymes in Pseudomonas putida strain H

Janke¹

1987

J. Basic Microbiol.

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A special approach was used to elucidate the "threshold" inducer concentration for coordinative de novo synthesis of phenol hydroxylase(s), catechol 2,3-dioxygenase and the 2-hydroxymuconic semialdehyde-metabolizing enzymes which initiate phenol catabolism in Pseudomonas putida strain H. It is based on cell-precultivation with glucose (as the carbon and energy source) in the presence of different concentrations of sodium salicylate which proved to be a potent non-metabolizable inducer in strain H of these enzymes. Subsequent estimation of the activity status of resting cell suspensions and cell-free extracts, respectively, prepared from those strain H cultures clearly revealed failing de novo synthesis of the mentioned phenol-degrading enzymes at salicylate concentrations lower than 0.2 mg/l.

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Influence of substrate concentration on the induction of amidases in herbicide degradation

Cited by 10 publications

References 10 publications

Constitutive mineralization of low concentrations of the herbicide linuron by aVariovoraxsp. strain

Constitutive mineralization of low concentrations of the herbicide linuron by aVariovoraxsp. strain

Aryl acylamidase from Rhodococcus erythropolis NCIB 12273

Use of salicylate to estimate the “threshold” inducer level for de novo synthesis of the phenol‐degrading enzymes in Pseudomonas putida strain H

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