Influence of streptozotocin- and alloxan-induced diabetes on the metabolism of glycosaminoglycans

Mohanam, Sanjeeva; Bose, S. M.

doi:10.1007/bf02642893

Cited by 9 publications

(2 citation statements)

References 37 publications

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“…Increase in relative weight of the kidney during diabetes has been reported by others. The degree of renal enlargement was correlated with the degree of glycemic control [25]. Curcumin, the active principle in turmeric, was more effective in ameliorating diabetic condition than whole turmeric ( Curcuma longa ) and attenuated kidney enlargement in streptozotocin-induced diabetic rats [26,27].…”

Section: Discussionmentioning

confidence: 99%

Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats

Kumar

Salimath

2014

J Diabetes Metab Disord

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BackgroundCurcumin known to have number of medicinal use and masked the fiber containing ukonan like active polysaccharide in turmeric and its pharmacological effect will be addressed on diabetic nephropathy particularly the glycoconjugates of extracellular components viz., glycoproteins and glycosaminoglycans - heparan sulfate (HS).MethodsMale Wistar rats were maintained on AIN-76 diet containing 10% spent turmeric and were grouped into control and STZ induced diabetes SFC/TFC and SFD/TFD, respectively. Diabetic status was monitored using blood and urine, and at the end, harvested kidneys were used to study the amelioration of glycoprotiens (collagen) and HS by enzymatic digestion, spectrophotometric, hydroxyproline and agarose electrophoretic methods.ResultsIn the present study spent turmeric (10%) fed diabetic rats showed improved glomerular filtration rate (50%), kidney enlargement (60%) and other glycoconjugate metabolism in kidney. Increased collagen content in diabetic group was observed by hydroxyproline estimation (24%) and periodic acid-Schiff’s (PAS) staining. Furthermore, elevated activities of enzymes involved in the synthesis and degradation of glycosaminoglycans (GAGs) were significantly lowered in spent turmeric fed diabetic group. Improvement in total GAGs (43%) and sulfate content (18%) followed by fractionation of GAGs using specific enzymes led to HS (28%) in the spent turmeric fed diabetic group, when compared to starch fed diabetic group and was further confirmed by electrophoresis of GAG.ConclusionThese results clearly indicate beneficial role of spent turmeric in controlling glycoconjugates such as glycoproteins and heparan sulfate related kidney complications during diabetes.

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Section: Discussionmentioning

confidence: 99%

Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats

Kumar

Salimath

2014

J Diabetes Metab Disord

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“…It was previously reported that STZ-induced diabetes has an effect on the cellular glycosaminoglycan (GAG) content and STZ-induced diabetes alters the synthesis and catabolism of GAG [ 44 ]. Also, it was found that the sulfation of heparan sulfate is reduced in the liver of STZ-induced diabetic rats [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-diabetic activity of different oils through their effect on arylsulfatases

Samarji

Balbaa

2014

J Diabetes Metab Disord

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BackgroundDiabetes mellitus (DM) is characterized by the overproduction of the reactive oxygen species which affects the integrity of the lysosomal membrane affecting lysosomal enzymes. The effect of these species is blocked by some natural products as antioxidants. In the current study, groups of normal and streptozotocin (STZ)-induced diabetic rats were treated by Nigella sativa (NS), olive and canola oils and subjected to the study of arylsulfatases as a model of lysosomal enzymes. The aim of the present study is to investigate the effect of STZ-induced diabetes on arylsulfatases in presence and absence of NS, olive and canola oils.MethodsDifferent groups of rats were induced by STZ, treated with different oils and compared to their corresponding control group. All groups were subjected for the assays of blood glucose, insulin, catalase and arylsulfatases. A comparative kinetic study of arylsulfatses was performed to detect the alteration of catalytic characterization.ResultsThe results demonstrated that diabetes causes a significant elevation in the level of hepatic arylsulfatase B and a significant reduction of hepatic catalase as an antioxidant enzyme. NS and olive oils returned catalase and arylsulfatase B activities back near to normal by fixing their catalytic properties. Furthermore, the maximum velocity of arylsulfatases A and B was significantly elevated in the induced diabetes, whereas their Km values were significantly changed. The treatment of diabetic rats by NS and olive oils reduced the degree of significance.ConclusionDiabetes induces significant alterations of the catalytic characters of arylsulfatases and some oils decrease this alteration through an antioxidant-mediated effect.

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Activities of Lysosomal Enzymes in Alloxan-Induced Diabetes in the Mouse

Witek

Rochon-Szmejchel²,

Stanisławska³

et al. 2017

Advances in Experimental Medicine and Biology

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The study investigated a panel of lysosomal enzymes in the liver and kidney tissues in alloxan-induced diabetes in the mouse. The mice were divided into six experimental groups receiving 10% alloxan at a dose of 50 and 75 mg/kg over a period of four, eight, and twelve days; each group was compared with controls receiving 0.9% NaCl. The findings were that diabetes induced by both doses of alloxan was accompanied by significant increases in the lysosomal activities of acid phosphatase and the glycosidases investigated: β-glucuronidase, β-galactosidase, β-glucosidase, and N-acetyl-hexosaminidase. The lysosomal enzyme activity in both liver and kidney cells peaked 12 days after onset of diabetes for most enzymes, at the time when hyperglycemia and hyperinsulinemia already started abating after their peak at 8 days into the course of diabetes. The enzyme activity was in most cases higher with the higher dose of alloxan and thus higher level of glycemia. Lysosomal enzymes degrade glycoconjugates, the molecules that are present in the basement membrane of endothelial cells where they contribute to capillary wall stability. Thus, enhanced activity of these enzymes could presage the progression of diabetic microangiopathy, atherosclerosis, and the development of microvascular complications.

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Influence of streptozotocin- and alloxan-induced diabetes on the metabolism of glycosaminoglycans

Cited by 9 publications

References 37 publications

Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats

Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats

Anti-diabetic activity of different oils through their effect on arylsulfatases

Activities of Lysosomal Enzymes in Alloxan-Induced Diabetes in the Mouse

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