Effect of spent turmeric on kidney glycoconjugates in streptozotocin-induced diabetic rats

Abstract

BackgroundCurcumin known to have number of medicinal use and masked the fiber containing ukonan like active polysaccharide in turmeric and its pharmacological effect will be addressed on diabetic nephropathy particularly the glycoconjugates of extracellular components viz., glycoproteins and glycosaminoglycans - heparan sulfate (HS).MethodsMale Wistar rats were maintained on AIN-76 diet containing 10% spent turmeric and were grouped into control and STZ induced diabetes SFC/TFC and SFD/TFD, respectively. Diabetic status was monitored using blood and urine, and at the end, harvested kidneys were used to study the amelioration of glycoprotiens (collagen) and HS by enzymatic digestion, spectrophotometric, hydroxyproline and agarose electrophoretic methods.ResultsIn the present study spent turmeric (10%) fed diabetic rats showed improved glomerular filtration rate (50%), kidney enlargement (60%) and other glycoconjugate metabolism in kidney. Increased collagen content in diabetic group was observed by hydroxyproline estimation (24%) and periodic acid-Schiff’s (PAS) staining. Furthermore, elevated activities of enzymes involved in the synthesis and degradation of glycosaminoglycans (GAGs) were significantly lowered in spent turmeric fed diabetic group. Improvement in total GAGs (43%) and sulfate content (18%) followed by fractionation of GAGs using specific enzymes led to HS (28%) in the spent turmeric fed diabetic group, when compared to starch fed diabetic group and was further confirmed by electrophoresis of GAG.ConclusionThese results clearly indicate beneficial role of spent turmeric in controlling glycoconjugates such as glycoproteins and heparan sulfate related kidney complications during diabetes.

Tanshinone IIA Ameliorates Streptozotocin-Induced Diabetic Nephropathy, Partly by Attenuating PERK Pathway-Induced Fibrosis

“…Therefore, we hypothesized that Tan IIA may be associated with the suppression According to a previous study, over-expression of TGF-β1 contributed to collagen deposition and GBM thickening. 28 Additionally, TGF-β1 can mediate tubulointerstitial fibrosis and renal hypertrophy in DN. 29 Our results showed that the diabetic state enhanced renal TGF-β1 expression, which is consistent with other reports.…”

Nephroprotective effects of Aralia taibaiensis in a high-fat diet-streptozotocin rat model of diabetic nephropathy

“…Prolonged hyperglycemia is known to impair kidney function and structure [ 16 , 17 ]. Early pathological changes in DN include kidney enlargement, thickening of glomerular and tubular basement membranes, and increased urine albumin excretion rate [ 18 ].…”

In vitro fermentation of glycosaminoglycans from mackerel fish waste and its role in modulating the antioxidant status and gut microbiota of high fat diet-fed C57BL/6 mice

“…[48][49][50] The utilization of e-GAGs by microbes through fermentation was confirmed using the well-established dye binding method (DMMB), which is very specific for GAGs. 51 The results showed a decreased content of e-GAGs, suggesting their utilization by microbes for their growth.…”