Influence of some parameters on the ability of Listeria monocytogenes, Listeria innocua, and Escherichia coli to form biofilms

Abstract

Aim: The present study was conducted to evaluate the capacity of Listeria monocytogenes (L.m), Listeria innocua (L.i), and Escherichia coli to form biofilms on polystyrene support under different parameters by performing crystal violet (CV) staining technique. Materials and Methods: Different suspensions were prepared with single strains and with multiple combinations of strains including two serogroups of L.m (IIa and IIb), L.i, and E. coli strains at different microbial load. Selected strains and combinations were grown in biofilms for 6 days attached to polystyrene microplates under aerobic and microaerophilic conditions. The evaluation of the power of adhesion and biofilm formation was determined by CV staining followed by the measurement of optical density at 24 h, 72 h, and 6 days incubation time with and without renewal of the culture medium. Results: All the strains tested, presented more or less adhesion power depending on the variation of the studied parameters as well as the ability to form multispecies biofilms. Their development is more important by renewing the culture medium and increasing the initial load of bacteria. The ability to adhere and form biofilms differs from one serogroup to another within the same species. In bacterial combination, strains and species of bacteria adopt different behaviors. Conclusion: The ability to form biofilms is a key factor in the persistence of tested strains in the environment. Our study showed that L.m, L.i, and E. coli could adhere to polystyrene and form biofilms under different conditions. More researches are necessary to understand the mechanisms of biofilm formation and the influence of different parameters in their development.

Role of Lactobacillus biofilms in Listeria monocytogenes adhesion to glass surfaces

“…This pattern has been previously documented in surfaces conditioned with P. fluorescens biofilms (Puga et al 2018). L. monocytogenes is a facultative microorganism capable of forming biofilms under oxygen restrictive conditions (Lezzoum-Atek et al 2019). The fact that L.…”

Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis

“…Although studies reported lactobacilli biofilm/biosurfactant activities against pathogens [10-13], few authors evaluated the inhibition of the initial adhesion of pathogens [16,17,23,33]. Some studies previously characterized the inhibition of the initial adhesion of certain pathogens, such as Gardnerella vaginalis, Prevotella bivia, Mobiluncus mulieris [23], Listeria monocytogenes [34], and Streptococcus mutans [17]. Recently, He et al [35] evaluated the probiotic activities of Lactobacillus species on the inhibition of the initial adhesion of several pathogens.…”