Influence of soluble suture factors on in vitro macrophage function

Uff, C. R.; Scott, A. D. N.; Pockley, A. Graham; Rw, Phillips

doi:10.1016/0142-9612(95)93852-5

Cited by 61 publications

(42 citation statements)

References 21 publications

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“…In the experiment without ABX treatment, a statistically significant difference between Ethilon II and Quill could be found only for E. faecium with p < 0.001 (halo size: Ethilon II, 0 mm [0-1]; Quill, 3 mm [2][3][4][5][6][7][8]). Between Quill and Vicryl, a statistically significant difference was determined for E. faecium and P. aeruginosa at p < 0.001 each.…”

Section: Resultsmentioning

confidence: 99%

“…7 After the suture is contaminated, local mechanisms of wound decontamination, mediated by granulocytic cell populations, become ineffective. 8 This situation is aggravated further by biofilm formation, 9 in which bacteria that are attached to a surface encapsulate themselves within a self-secreted extracellular polymeric slime matrix composed of polysaccharides, proteins, and nucleic acids. 10 In more than 60% of clinical infections, bacteria protect themselves by hiding in such a biofilm.…”

mentioning

confidence: 99%

“…around Vicryl was reduced by a factor of 1.5, from 12 mm (11-16) to 8 mm (5)(6)(7)(8)(9)(10)(11)(12)(13)(14). With E. coli and E. faecium, but also with S. aureus, even under a long and high concentration treatment, bacterial presence on the sutures could only be partly reduced.…”

mentioning

confidence: 99%

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Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Dhom

Bloes

Peschel

et al. 2016

Journal Orthopaedic Research

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Contaminated suture material plays an important role in the physiopathology of surgical site infections. Recently, suture material has been developed characterized by barbs projecting from a monofilament base. Claimed advantages for barbed sutures are a shortened wound closure time and reduced maximum wound tension. It has also been suggested that these sutures would be advantageous microbiologically. The aim of this study was to test the microbiological characteristics of the barbed Quill in comparison to the monofilament Ethilon II and the braided sutures Vicryl and triclosan-coated Vicryl Plus. In our study, sutures were cultivated on color-change agar with Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Escherichia coli, and Pseudomonas aeruginosa and the halo size was measured. In a second study arm with longer cultivation bacterial growth was followed by antibiotic treatment. Ethilon II and Quill showed good comparable results, whereas large halos were found around Vicryl. Vicryl Plus results depended on triclosan sensitivity. After longer bacterial cultivation and antibiotic treatment, halos were up to 3.6 times smaller on Quill than on Vicryl (p < 0.001), but 1.4 times larger than on Ethilon II (p < 0.001) regarding S. aureus. Confocal microscopy analysis showed bacterial colonization between the braided filaments on Vicryl and beneath the barbs on Quill. From a microbiological perspective, barbed sutures can be recommended in aseptic surgery, but should only be used carefully in septic surgery.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Dhom

Bloes

Peschel

et al. 2016

Journal Orthopaedic Research

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“…Uff et al [51] examined the effects of soluble factors from sutures on macrophage activation (e.g., attachment, phagacytosis and the production of lysozyme and proinflammatory tumor necrosis factor (TNF)) in vitro. Soluble factors were acquired by subjecting sterile sutures to ultrasound for 1 h in detergent.…”

Section: Concernsmentioning

confidence: 99%

Silk-based biomaterials

et al. 2003

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Silk from the silkworm, Bombyx mori, has been used as biomedical suture material for centuries. The unique mechanical properties of these fibers provided important clinical repair options for many applications. During the past 20 years, some biocompatibility problems have been reported for silkworm silk; however, contamination from residual sericin (glue-like proteins) was the likely cause. More recent studies with well-defined silkworm silk fibers and films suggest that the core silk fibroin fibers exhibit comparable biocompatibility in vitro and in vivo with other commonly used biomaterials such as polylactic acid and collagen. Furthermore, the unique mechanical properties of the silk fibers, the diversity of side chain chemistries for 'decoration' with growth and adhesion factors, and the ability to genetically tailor the protein provide additional rationale for the exploration of this family of fibrous proteins for biomaterial applications. For example, in designing scaffolds for tissue engineering these properties are particularly relevant and recent results with bone and ligament formation in vitro support the potential role for this biomaterial in future applications. To date, studies with silks to address biomaterial and matrix scaffold needs have focused on silkworm silk. With the diversity of silk-like fibrous proteins from spiders and insects, a range of native or bioengineered variants can be expected for application to a diverse set of clinical needs. r

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“…PDO is used in the medical field for making suture wires due to its biocompatibility, flexibility, and elasticity [4,5]. No long term cytotoxicity has been observed with PDO implanted for upto 6 months in mice [6].…”

Section: Introductionmentioning

confidence: 99%

Safety and Efficacy of Polydioxanone Nano-Fibers as Anti-Inflammatory Agents

Gollapudi¹

2014

J Nanomedine Biotherapeutic Discov

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PDOPDO nano-fibers were a kind gift of Dongbang Acupuncture Ltd, Korea. Phagocytosis assayPhagocytosis was assessed by flow cytometry using pHrodo AbstractThe use of biodegradable polydioxanone (PDO) nano-fibers for various purposes is increasing but the effects of these fibers on immune responses are not well understood. We examined the potential stimulatory and inhibitory effects of PDO coated fibers on immune cell functions in vitro in humans and in vivo in mice. Short term in vitro exposure of human blood to PDO did not perturb the phagocytic function of human monocytes and neutrophils. In contrast lymphocytes exhibited an increased proliferative function in response to polyclonal T cell mitogen, PHA. However, cytokine secretion by monocytes and lymphocytes as well as NK cell cytotoxic effector cell functions were undisturbed by PDO exposure. Long term in vivo exposure to PDO had no effect on dendritic cells activation, cytokine secretion and T regulatory induction. Injection of PDO into mice with rheumatoid arthritis suggested that PDO nano-fibers tend to be anti-inflammatory as an increase in IL-10 was observed in the PDO treated groups. In spite of this the arthritic score and TNF-α and IFN-γ levels were not significantly different between PDO-treated and untreated rheumatoid arthritis induced mice. In conclusion, PDO nano-fibers have no significant adverse effect on immune functions and tend to induce anti-inflammatory responses upon long term exposure in vivo.

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Influence of soluble suture factors on in vitro macrophage function

Cited by 61 publications

References 21 publications

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Silk-based biomaterials

Safety and Efficacy of Polydioxanone Nano-Fibers as Anti-Inflammatory Agents

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