Influence of smoking habit on the frequency of micronuclei in human lymphocytes by the cytokinesis block method

Tomanin, Rosella; Ballarin, Cinzia; Nardini, Barbara; Mastrangelo, Giuseppe; Sarto, Fabio

doi:10.1093/mutage/6.2.123

Cited by 68 publications

(29 citation statements)

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“…Several factors can influence the frequency of MN in the cell, such as age [2,16,19,33,36,47] [30] showed that any chromosome could be present in MN. The mechanism by which the anesthetics induce DNA damage is still controversial.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Araujo¹,

Silva-Grecco²,

Bisinotto³

et al. 2013

J Anesthesiol Clin Sci

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Exposure to certain chemical agents causes damage to the genetic material. There is controversy about the genotoxic and/or mutagenic effect caused by acute or chronic anesthetics exposure. The aim of this research was to assess and compare the frequency of micronuclei (MN) in professionals working in operating rooms and other hospital areas. The genotoxic and cytotoxic effects were evaluated in lymphocytes using the micronucleus test and the proliferative index, respectively. We examined peripheral blood lymphocyte cultures from 30 operating room professionals (exposed group) and 30 professionals non-exposed in other hospital areas of the same hospital (control group). There was no statistically significant difference in proliferative index between the groups. Nevertheless, there was an increase in MN frequency in binucleated (BN) cells in the exposed group (p=0.0003) compared to the control group. Moreover, there was a statistically significant difference between genders (p=0.0187), and the frequency of MN in BN cells from women was higher than in men. Therefore, gender influenced the frequency of MN. The age and period of working time in an operating room influenced the MN frequency only in women professionals. Thereby, there was a genotoxic effect in occupationally exposed professionals, and the micronucleus test could be used in the biomonitoring of human populations for evaluating the risk of developing cancer.

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Section: Discussionmentioning

confidence: 99%

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Araujo¹,

Silva-Grecco²,

Bisinotto³

et al. 2013

J Anesthesiol Clin Sci

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“…In fact, although cigarette smoke contains many carcinogens and mutagens, the studies published to date that link smoking habits to MN frequency are rather controversial, with some showing a positive effect [26][27][28] and others showing no significance or even a negative effect [29][30][31]. The mechanisms underlying this phenomenon need to be studied in more detail.…”

Section: Discussionmentioning

confidence: 99%

Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study

Morimoto

2010

Environ Health Prev Med

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Objective Results from previous studies suggest that alcohol consumption can be genotoxic on peripheral lymphocytes. The aim of our study was to examine the association of alcohol consumption and its genotoxic effect on hematopoietic stem cells in vivo. Methods We investigated 156 healthy Japanese males in a cross-sectional study. Lifestyles, including alcohol drinking behavior and cigarette smoking status, were investigated by means of a self-completed questionnaire. Polymorphisms of ADH1B and ALDH2 were identified by PCR-restriction fragment length polymorphism (RFLP) analysis. The presence of micronuclei in transferrin-positive reticulocytes (MN-RET) was detected with a single-laser flow cytometer. Associations between the genetic polymorphisms, lifestyle factors, and MN-RET frequency were statistically analyzed. Results We found a significant difference in the mean frequencies of MN-RET between habitual drinkers and non-habitual drinkers (P = 0.043), and between the ALDH2*1/*1 and ALDH2*2/*2 genotype (P = 0.015). The ADH1B*2 and ALDH2*2 haplotype was estimated to have a significantly higher influence on MN-RET frequency than the ADH1B*2 and ALDH2*1 haplotype (P = 0.00035), and the frequency of alcohol consumption played a significant role in MN-RET frequency on the background of the ADH1B*2 and ALDH2*1 haplotype (P = 0.012). Conclusion The results of our study suggest a possible association between the ADH1B and ALDH2 polymorphism and the genotoxic effects of alcohol drinking on hematopoietic stem cells.

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“…Općenito uzevši, podaci o doprinosu navike pušenja porastu vrijednosti parametara MN-testa su kontradiktorni. Dok rezultati nekih istraživanja upućuju na štetan utjecaj navike pušenja i porast broja MN u pušača (13,28,(57)(58)(59)(60), dio istraživanja nije dokazao takvu povezanost (16,37,38,61). Rezultati opsežnog istraživanja provedenog u okviru projekta HUMN (62) ne pokazuju statistički značajan doprinos navike pušenja vrijednostima parametara MN-testa u "prosječnih" pušača, već samo u onih koji puše više od 30 cigareta na dan.…”

Section: Raspravaunclassified

Normal and Cut-Off Values of the Cytokinesis-Block Micronucleus Assay on Peripheral Blood Lymphocytes in the Croatian General Population

Kopjar

Kašuba

Milić

et al. 2010

Archives of Industrial Hygiene and Toxicology

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Institut za medicinska istraživanja i medicinu rada, Zagreb, HrvatskaPrimljeno u veljači 2010. Prihvaćeno u ožujku 2010.Mikronukleus (MN) test na limfocitima periferne krvi jedna je od najvažnijih metoda koje se primjenjuju u citogenetičkom nadzoru. Osnovni preduvjet za primjenu nekog testa u svrhu nadzora profesionalno izloženih populacija jest poznavanje normalnih vrijednosti promatranoga biološkog pokazatelja (biomarkera) u kontrolnoj populaciji. Baze podataka na razini opće populacije moraju se redovito obnavljati novim podacima. Cilj ovog istraživanja bio je utvrditi normalne i granične vrijednosti MN-testa na limfocitima periferne krvi 200 zdravih ispitanika obaju spolova iz opće populacije Republike Hrvatske te ispitati koji čimbenici pridonose spontanom nastanku MN. Na razini istražene populacije utvrđeno je prosječno (6,90±3,32) MN (medijan 7 MN), dok je raspon pojedinačnih vrijednosti iznosio 0 do 18 MN u 1000 binuklearnih stanica. Gornja granična vrijednost dobivena izračunavanjem 95. percentila za cjelokupnu promatranu populaciju iznosi 12,5 MN na 1000 limfocita. Utvrđeno je da na spontani nastanak MN utječu spol, dob i navika pušenja. Žene u prosjeku imaju više vrijednosti svih parametara MN-testa od muškaraca, a u njih je bio i naglašeniji porast vrijednosti citogenetičkog nalaza zbog navike pušenja. Kako su literaturni podaci o utjecaju pušenja cigareta na nastanak MN kontradiktorni, planiran je nastavak istraživanja radi razjašnjavanja utjecaja dnevno utrošenog broja cigareta i ukupnog trajanja pušačkog staža na vrijednosti parametara MN-testa. Usporedba rezultata s literaturnim podacima potvrdila je da su dobivene vrijednosti u skladu s vrijednostima MN-testa zabilježenim na općoj populaciji u drugim svjetskim laboratorijima. Normalne i granične vrijednosti MN-testa utvrđene u ovome istraživanju poslužit će kao osnova za usporedbu i tumačenje nalaza MN-testa u ispitanika izloženih populacija te daljnju nadogradnju laboratorijske baze podataka. KLJUČNE RIJEČI: citogenetički nadzor, dob, nestabilnost genoma, pušenje cigareta, rizik, spol, spontani nastanak mikronukleusaBiološki nadzor (engl. biomonitoring) važan je dio zdravstvenog nadzora nad ispitanicima koji su profesionalno izloženi različitim fizikalnim i kemijskim mutagenima i/ili kancerogenima. Zasniva se na mjerenju (ili utvrđivanju učestalosti) različitih bioloških pokazatelja koji upućuju na najranije, još popravljive biološke učinke, koji prethode pojavi zloćudnih i/ili drugih bolesti. Primjenom biljega izloženosti, učinka i sklonosti (engl. susceptibility) na razini proučavanih populacija možemo utvrditi kakve su razine izloženosti štetnostima na radnim mjestima, je li izloženost rezultirala mjerljivim učinkom (primjerice oštećenjem molekule DNA) te otkriti ispitanike s većom osjetljivosti genoma koji su pod povišenim * svi autori su jednakovrijedni Kopjar N, et al.

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Influence of smoking habit on the frequency of micronuclei in human lymphocytes by the cytokinesis block method

Cited by 68 publications

References 0 publications

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Genotoxic effects of anesthetics in operating room personnel evaluated by micronucleus test

Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes: a preliminary study

Normal and Cut-Off Values of the Cytokinesis-Block Micronucleus Assay on Peripheral Blood Lymphocytes in the Croatian General Population

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