Influence of silver nanoparticles on metabolism and toxicity of moulds

Abstract

The unique antimicrobial features of silver nanoparticles (AgNPs) are commonly applied in innumerable products. The lack of published studies on the mechanisms of AgNPs action on fungi resulted in identification of the aim of this study, which was: the determination of the influence of AgNPs on the mould cytotoxicity for swine kidney cells (MTT test) and the production of selected mycotoxins, organic acids, extracellular enzymes by moulds. The conducted study had shown that silver nanoparticles can change the metabolism and toxicity of moulds. AgNPs decrease the mycotoxin production of Aspergillus sp. (81-96%) and reduce mould cytotoxicity (50-75%). AgNPs influence the organic acid production of A. niger and P. chrysogenum by decreasing their concentration (especially of the oxalic and citric acid). Also, a change in the extracellular enzyme profile of A. niger and P. chrysogenum was observed, however, the total enzymatic activity was increased.

Morphology, Photocatalytic and Antimicrobial Properties of TiO2 Modified with Mono- and Bimetallic Copper, Platinum and Silver Nanoparticles

“…The highest activity was obtained for silver-modified nanocomposites obtained by chemical reduction, characterized by small particle size. The improvement of antimicrobial properties of metal-modified titanium(IV) oxide is possible due to the synergism of the antimicrobial properties of metals and reactive oxygen species generation on the TiO 2 surface [3,79,80,81,82,83]. For gram-negative strains, silver nanoparticles are able to absorb and accumulate on the outer cell wall leading to the disintegration of the lipid bilayer and an increase in its permeability.…”

Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins

“…The findings indicate that Ag NPs inhibit fungal growth as well as morphological and metabolic changes [ 85 , 86 ]. For instance, the application of 45 ppm Ag NPs caused a decrease of organic acid (oxalic, maleic, and citric acid) production, mycotoxin production (up to 80%), and changes in the enzymatic profile in Aspergillus niger and Penicillium chrysogenum [ 85 ]. All the studies reviewed so far, however, suffer from the fact that the interaction of NPs with the individual components of the fungi cells has not been investigated yet.…”

Antifungal Nano-Therapy in Veterinary Medicine: Current Status and Future Prospects

“…The nanosized silver can inhibit the growth of Fusaium sp. at very low concentrations (<100 ppm) [ 16 , 60 ] and led to decreased mycotoxin production [ 1 , 61 ]. Also, Kischkel et al [ 37 ] observed the antifungal activity of the AgNPs against C. albicans , F. oxysporum and M. canis .…”